Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Arthrobacter arilaitensis (strain DSM 16368 / CIP 108037 / JCM 13566 / Re117)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi481 – 4811MagnesiumUniRule annotation
Metal bindingi487 – 4871Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi490 – 4901MagnesiumUniRule annotation
Metal bindingi491 – 4911MagnesiumUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciAARI861360:GI6T-1216-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotationSAAS annotation (EC:6.1.1.20UniRule annotationSAAS annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Gene namesi
Name:pheTUniRule annotationImported
Ordered Locus Names:AARI_11310Imported
OrganismiArthrobacter arilaitensis (strain DSM 16368 / CIP 108037 / JCM 13566 / Re117)Imported
Taxonomic identifieri861360 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
ProteomesiUP000006878 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 170129tRNA-bindingUniRule annotationAdd
BLAST
Domaini428 – 50376B5UniRule annotationAdd
BLAST
Domaini754 – 84794FDX-ACBUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000292086.
KOiK01890.
OMAiRQYGLHT.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E1VUN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIPLSWLRE YAQVPADASA EDVMADLVKV GLEEEDVHRP SDELSGPIVV
60 70 80 90 100
GQVLSLEKEV ASNGKTINWC QVRVVPEGTE QTLTGKGIDP TGVQGIVCGA
110 120 130 140 150
HNFVEGDKVV VTLPGAVLPG DFKISPRKTY GHTSAGMIAS SRELGIGEDH
160 170 180 190 200
DGIMVLSNYG LDPEVGTDVL ALFGLDDQAA EINVTPDRGY CFSIRGVARE
210 220 230 240 250
YALATGTSFT DPASIVTVSD ASEAGHEVVL ADAAPIYGVP GCTRFVTREV
260 270 280 290 300
TGIDPTRPTP RWMASRLQLA GMRSISLVVD ISNYVMLELG APLHFYDADK
310 320 330 340 350
LTSAITVRRA NSGEKLTTLD DKERELSVED LLITDESGAI GIAGVMGGAA
360 370 380 390 400
TEVSDSTSRV LIEAAHFDTV SIGRSRRRHK LPSEASKRFE RGVDPRIMQI
410 420 430 440 450
AAQRAVNLLV ELAGGTETAK ATDAGAVPAD TQIQLPAGFA GALIGVDYTD
460 470 480 490 500
EQTISALEGI GAAVQQNAEG FLVTAPSWRP DLDIKEDLVE EVARVIGYDK
510 520 530 540 550
IPATLPVAPP GRGLTRTQSQ RRRLLQGLAD AGLTEVLNYP FVSQAQNATF
560 570 580 590 600
GAAQAGTEVK AVSLANPISK EFRFLRTSLL PGLLETARRN IGRGFRDLAL
610 620 630 640 650
YEGGLVFLPG QQLGSSVLPP LGAKPSDEVL AELFNGVPNQ PWHLAAVFTG
660 670 680 690 700
HEASAAAGFA PRAWDWADAL DAAHNVADIL GVELEVAQGS HQAFHPGRVA
710 720 730 740 750
ALKLAGEVVG YAGELHPQLL KDQDLPARTV AMELDAAALM SAAPAVVVAQ
760 770 780 790 800
QHLSSQPLAT QDVALVVDQD VVAGDVLATL REGAGELLED IALFDVYQGQ
810 820 830 840
GIEEGKKSLA FGLRFRAADR TLTADEASEV RAAAVDAAAK KFGATQR
Length:847
Mass (Da):89,599
Last modified:November 30, 2010 - v1
Checksum:i4A71A5850EF79367
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ311875 Genomic DNA. Translation: CBT75342.1.
RefSeqiWP_013348486.1. NC_014550.1.
YP_003916313.1. NC_014550.1.

Genome annotation databases

EnsemblBacteriaiCBT75342; CBT75342; AARI_11310.
KEGGiaai:AARI_11310.
PATRICi42168925. VBIArtAri166201_1213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FQ311875 Genomic DNA. Translation: CBT75342.1.
RefSeqiWP_013348486.1. NC_014550.1.
YP_003916313.1. NC_014550.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBT75342; CBT75342; AARI_11310.
KEGGiaai:AARI_11310.
PATRICi42168925. VBIArtAri166201_1213.

Phylogenomic databases

HOGENOMiHOG000292086.
KOiK01890.
OMAiRQYGLHT.

Enzyme and pathway databases

BioCyciAARI861360:GI6T-1216-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The arthrobacter arilaitensis Re117 genome sequence reveals its genetic adaptation to the surface of cheese."
    Monnet C., Loux V., Gibrat J.F., Spinnler E., Barbe V., Vacherie B., Gavory F., Gourbeyre E., Siguier P., Chandler M., Elleuch R., Irlinger F., Vallaeys T.
    PLoS ONE 5:e15489-e15489(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 16368 / CIP 108037 / JCM 13566 / Re117Imported.
  2. "Complete genome sequence of Arthrobacter arilaitensis (strain DSM 16368 / CIP 108037 / JCM 13566 / Re117)."
    Genoscope.
    Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16368 / CIP 108037 / JCM 13566 / Re117Imported.

Entry informationi

Entry nameiE1VUN9_ARTAR
AccessioniPrimary (citable) accession number: E1VUN9
Entry historyi
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: April 29, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.