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E1VUN9

- E1VUN9_ARTAR

UniProt

E1VUN9 - E1VUN9_ARTAR

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Arthrobacter arilaitensis (strain DSM 16368 / CIP 108037 / JCM 13566 / Re117)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi481 – 4811MagnesiumUniRule annotation
    Metal bindingi487 – 4871Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi490 – 4901MagnesiumUniRule annotation
    Metal bindingi491 – 4911MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciAARI861360:GI6T-1216-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Gene namesi
    Name:pheTUniRule annotationImported
    Ordered Locus Names:AARI_11310Imported
    OrganismiArthrobacter arilaitensis (strain DSM 16368 / CIP 108037 / JCM 13566 / Re117)Imported
    Taxonomic identifieri861360 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter
    ProteomesiUP000006878: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 170129tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini428 – 50376B5UniRule annotationAdd
    BLAST
    Domaini754 – 84794FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation
    Contains B5 domain.SAAS annotation
    Contains Bdomain.SAAS annotation
    Contains FDX-ACB domain.SAAS annotation
    Contains tRNA-binding domain.SAAS annotation

    Phylogenomic databases

    HOGENOMiHOG000292086.
    KOiK01890.
    OMAiHPGRTAQ.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E1VUN9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIPLSWLRE YAQVPADASA EDVMADLVKV GLEEEDVHRP SDELSGPIVV    50
    GQVLSLEKEV ASNGKTINWC QVRVVPEGTE QTLTGKGIDP TGVQGIVCGA 100
    HNFVEGDKVV VTLPGAVLPG DFKISPRKTY GHTSAGMIAS SRELGIGEDH 150
    DGIMVLSNYG LDPEVGTDVL ALFGLDDQAA EINVTPDRGY CFSIRGVARE 200
    YALATGTSFT DPASIVTVSD ASEAGHEVVL ADAAPIYGVP GCTRFVTREV 250
    TGIDPTRPTP RWMASRLQLA GMRSISLVVD ISNYVMLELG APLHFYDADK 300
    LTSAITVRRA NSGEKLTTLD DKERELSVED LLITDESGAI GIAGVMGGAA 350
    TEVSDSTSRV LIEAAHFDTV SIGRSRRRHK LPSEASKRFE RGVDPRIMQI 400
    AAQRAVNLLV ELAGGTETAK ATDAGAVPAD TQIQLPAGFA GALIGVDYTD 450
    EQTISALEGI GAAVQQNAEG FLVTAPSWRP DLDIKEDLVE EVARVIGYDK 500
    IPATLPVAPP GRGLTRTQSQ RRRLLQGLAD AGLTEVLNYP FVSQAQNATF 550
    GAAQAGTEVK AVSLANPISK EFRFLRTSLL PGLLETARRN IGRGFRDLAL 600
    YEGGLVFLPG QQLGSSVLPP LGAKPSDEVL AELFNGVPNQ PWHLAAVFTG 650
    HEASAAAGFA PRAWDWADAL DAAHNVADIL GVELEVAQGS HQAFHPGRVA 700
    ALKLAGEVVG YAGELHPQLL KDQDLPARTV AMELDAAALM SAAPAVVVAQ 750
    QHLSSQPLAT QDVALVVDQD VVAGDVLATL REGAGELLED IALFDVYQGQ 800
    GIEEGKKSLA FGLRFRAADR TLTADEASEV RAAAVDAAAK KFGATQR 847
    Length:847
    Mass (Da):89,599
    Last modified:November 30, 2010 - v1
    Checksum:i4A71A5850EF79367
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FQ311875 Genomic DNA. Translation: CBT75342.1.
    RefSeqiYP_003916313.1. NC_014550.1.

    Genome annotation databases

    EnsemblBacteriaiCBT75342; CBT75342; AARI_11310.
    GeneIDi9794204.
    KEGGiaai:AARI_11310.
    PATRICi42168925. VBIArtAri166201_1213.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FQ311875 Genomic DNA. Translation: CBT75342.1 .
    RefSeqi YP_003916313.1. NC_014550.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBT75342 ; CBT75342 ; AARI_11310 .
    GeneIDi 9794204.
    KEGGi aai:AARI_11310.
    PATRICi 42168925. VBIArtAri166201_1213.

    Phylogenomic databases

    HOGENOMi HOG000292086.
    KOi K01890.
    OMAi HPGRTAQ.

    Enzyme and pathway databases

    BioCyci AARI861360:GI6T-1216-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Arthrobacter arilaitensis Re117 genome sequence reveals its genetic adaptation to the surface of cheese."
      Monnet C., Loux V., Gibrat J.F., Spinnler E., Barbe V., Vacherie B., Gavory F., Gourbeyre E., Siguier P., Chandler M., Elleuch R., Irlinger F., Vallaeys T.
      PLoS ONE 5:E15489-E15489(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM 16368 / CIP 108037 / JCM 13566 / Re117Imported.
    2. "Complete genome sequence of Arthrobacter arilaitensis (strain DSM 16368 / CIP 108037 / JCM 13566 / Re117)."
      Genoscope.
      Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 16368 / CIP 108037 / JCM 13566 / Re117Imported.

    Entry informationi

    Entry nameiE1VUN9_ARTAR
    AccessioniPrimary (citable) accession number: E1VUN9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 30, 2010
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3