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E1VH18 (E1VH18_9GAMM) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103
Short name=Fapy-DNA glycosylase HAMAP-Rule MF_00103
EC=3.2.2.23 HAMAP-Rule MF_00103
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM HAMAP-Rule MF_00103
Gene names
Name:nei EMBL CBL44110.1
Synonyms:fpg HAMAP-Rule MF_00103, mutM HAMAP-Rule MF_00103
ORF Names:HDN1F_05270 EMBL CBL44110.1
Organismgamma proteobacterium HdN1 EMBL CBL44110.1
Taxonomic identifier83406 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria ›

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103 SAAS SAAS015886

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103 SAAS SAAS015886

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103 SAAS SAAS000191

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00103 SAAS SAAS015886

Sequence similarities

Belongs to the FPG family. HAMAP-Rule MF_00103

Contains 1 FPG-type zinc finger. HAMAP-Rule MF_00103 SAAS SAAS015886

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Zinc finger236 – 27035FPG-type By similarity HAMAP-Rule MF_00103

Sites

Active site21Schiff-base intermediate with DNA By similarity HAMAP-Rule MF_00103
Active site31Proton donor By similarity HAMAP-Rule MF_00103
Active site571Proton donor; for beta-elimination activity By similarity HAMAP-Rule MF_00103
Active site2601Proton donor; for delta-elimination activity By similarity HAMAP-Rule MF_00103
Binding site901DNA By similarity HAMAP-Rule MF_00103
Binding site1091DNA By similarity HAMAP-Rule MF_00103
Binding site1511DNA By similarity HAMAP-Rule MF_00103

Sequences

Sequence LengthMass (Da)Tools
E1VH18 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: 9A3E60F9B82DC4A7

FASTA27030,390
        10         20         30         40         50         60 
MPELPEVETT RRGILPHIQG LRVQQVIVRQ PKLRWPVSPE LVQLAGEPLR DIARRGKYLL 

        70         80         90        100        110        120 
FSFDRGIVLA HLGMSGSLRI LESEKPADAH DHLDWVFENG ITLRYHDPRR FGAWLWWDGE 

       130        140        150        160        170        180 
STGHPLLESL GPEPLEPDFT AQYLYRTSRG RKVPVKSFVM NSHVVVGVGN IYANEALFSA 

       190        200        210        220        230        240 
GISPLRAAGR ISLERYQRLV ESIRKILEDS IRQGGTTLRD FVGGDGKPGY FQQTLQVYGR 

       250        260        270 
AGAPCLKCGE SLCEIRLGQR TTVFCKVCQT

« Hide

References

[1]"Alkane degradation by a new type of denitrifying bacterium with possible involvement of the electron acceptor in substrate activation."
Widdel F., Rabus R., Grundmann O., Werner I., Schreiber F., Ehrenreich P., Behrends A., Wilkes H., Kube M., Reinhardt R., Zedelius J.
Environ. Microbiol. 0:0-0(2010)
Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FP929140 Genomic DNA. Translation: CBL44110.1.
RefSeqYP_003809774.1. NC_014366.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBL44110; CBL44110; HDN1F_05270.
GeneID9703439.
KEGGgpb:HDN1F_05270.
PATRIC42343801. VBIGamPro61291_0572.

Phylogenomic databases

HOGENOMHOG000020881.
KOK10563.

Enzyme and pathway databases

BioCycGPRO83406:GIWA-528-MONOMER.

Family and domain databases

HAMAPMF_00103. Fapy_DNA_glycosyl.
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF81624. Form_DNAglyc_cat. 1 hit.
SSF46946. Ribosomal_H2TH. 1 hit.
TIGRFAMsTIGR00577. fpg. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE1VH18_9GAMM
AccessionPrimary (citable) accession number: E1VH18
Entry history
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: May 1, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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