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Protein

Ectoine-binding periplasmic protein TeaA

Gene

teaA

Organism
Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite ATP-independent periplasmic (TRAP) transport system TeaABC involved in the uptake of ectoine and hydroxyectoine in response to osmotic upshock. Probably functions as a recovery system for synthesized ectoine that leaks out of the cell. Binds ectoine with high affinity. Affinity for hydroxyectoine is approximately 20-fold lower.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34Ectoine1
Binding sitei169Ectoine1
Binding sitei209Ectoine1
Binding sitei213Ectoine1
Binding sitei234Ectoine1

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.56.2.1. the tripartite atp-independent periplasmic transporter (trap-t) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ectoine-binding periplasmic protein TeaA
Gene namesi
Name:teaA
Ordered Locus Names:HELO_4274
OrganismiHalomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)
Taxonomic identifieri768066 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaOceanospirillalesHalomonadaceaeHalomonas
Proteomesi
  • UP000008707 Componenti: Chromosome

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Deletion abolishes accumulation of ectoine from the medium.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273 – 275GLS → AAA: Strongly biased toward the open conformation. Has lower affinity for ectoine. Does not affect the structure of the substrate-binding site. 1 Publication3
Mutagenesisi273G → P: Conformationally unbiased. Has wild-type affinity for ectoine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000042882826 – 341Ectoine-binding periplasmic protein TeaAAdd BLAST316

Interactioni

Subunit structurei

Monomer. The complex comprises the extracytoplasmic solute receptor protein TeaA, and the two transmembrane proteins TeaB and TeaC.2 Publications

Protein-protein interaction databases

STRINGi768066.HELO_4274.

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 31Combined sources5
Helixi39 – 53Combined sources15
Beta strandi54 – 56Combined sources3
Beta strandi58 – 62Combined sources5
Turni64 – 67Combined sources4
Helixi73 – 78Combined sources6
Beta strandi83 – 87Combined sources5
Helixi89 – 92Combined sources4
Turni93 – 95Combined sources3
Helixi97 – 103Combined sources7
Helixi112 – 121Combined sources10
Helixi123 – 126Combined sources4
Helixi128 – 134Combined sources7
Turni135 – 137Combined sources3
Beta strandi138 – 155Combined sources18
Helixi160 – 163Combined sources4
Beta strandi167 – 170Combined sources4
Helixi174 – 183Combined sources10
Beta strandi186 – 189Combined sources4
Helixi192 – 194Combined sources3
Helixi195 – 200Combined sources6
Beta strandi205 – 210Combined sources6
Helixi211 – 216Combined sources6
Helixi219 – 221Combined sources3
Beta strandi225 – 228Combined sources4
Beta strandi233 – 241Combined sources9
Helixi242 – 247Combined sources6
Helixi250 – 271Combined sources22
Helixi274 – 285Combined sources12
Beta strandi290 – 293Combined sources4
Helixi296 – 303Combined sources8
Helixi306 – 334Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VPNX-ray1.55A/B26-341[»]
2VPOX-ray1.80A/B26-341[»]
3GYYX-ray2.20A/B/C/D1-341[»]
ProteinModelPortaliE1VBK1.
SMRiE1VBK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108KJ1. Bacteria.
COG1638. LUCA.
HOGENOMiHOG000224244.
OMAiHEYQIRI.
OrthoDBiPOG091H03NF.

Family and domain databases

InterProiIPR018389. TRAP_DctP/TeaA.
[Graphical view]
PfamiPF03480. DctP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E1VBK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAYKLLTTA SIGALMLGMS TAAYSDNWRY AHEEYEGDVQ DVFAQAFKGY
60 70 80 90 100
VEDNSDHTVQ VYRFGELGES DDIMEQTQNG ILQFVNQSPG FTGSLIPSAQ
110 120 130 140 150
IFFIPYLMPT DMDTVLEFFD ESKAINEMFP KLYAEHGLEL LKMYPEGEMV
160 170 180 190 200
VTADEPITSP EDFDNKKIRT MTNPLLAETY KAFGATPTPL PWGEVYGGLQ
210 220 230 240 250
TGIIDGQENP IFWIESGGLY EVSPNLTFTS HGWFTTAMMA NQDFYEGLSE
260 270 280 290 300
EDQQLVQDAA DAAYDHTIEH IKGLSEESLE KIKAASDEVT VTRLNDEQIQ
310 320 330 340
AFKERAPQVE EKFIEMTGEQ GQELLDQFKA DLKAVQSESE G
Length:341
Mass (Da):38,265
Last modified:November 30, 2010 - v1
Checksum:iE632B3C8EA9E5A11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061646 Genomic DNA. Translation: AAL29684.1.
FN869568 Genomic DNA. Translation: CBV44158.1.
RefSeqiWP_013334028.1. NC_014532.1.

Genome annotation databases

EnsemblBacteriaiCBV44158; CBV44158; HELO_4274.
KEGGihel:HELO_4274.
PATRICi42358222. VBIHalElo161731_3511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061646 Genomic DNA. Translation: AAL29684.1.
FN869568 Genomic DNA. Translation: CBV44158.1.
RefSeqiWP_013334028.1. NC_014532.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VPNX-ray1.55A/B26-341[»]
2VPOX-ray1.80A/B26-341[»]
3GYYX-ray2.20A/B/C/D1-341[»]
ProteinModelPortaliE1VBK1.
SMRiE1VBK1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi768066.HELO_4274.

Protein family/group databases

TCDBi2.A.56.2.1. the tripartite atp-independent periplasmic transporter (trap-t) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBV44158; CBV44158; HELO_4274.
KEGGihel:HELO_4274.
PATRICi42358222. VBIHalElo161731_3511.

Phylogenomic databases

eggNOGiENOG4108KJ1. Bacteria.
COG1638. LUCA.
HOGENOMiHOG000224244.
OMAiHEYQIRI.
OrthoDBiPOG091H03NF.

Family and domain databases

InterProiIPR018389. TRAP_DctP/TeaA.
[Graphical view]
PfamiPF03480. DctP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTEAA_HALED
AccessioniPrimary (citable) accession number: E1VBK1
Secondary accession number(s): Q8VPB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: November 30, 2010
Last modified: November 2, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The ectoine/TeaA complex displays a closed conformation (PubMed:18702523). Interaction with the transmembrane proteins induces opening of TeaA, which facilitates substrate release (PubMed:22084072).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.