Elongation factor 4 - Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

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E1V4G8 (E1V4G8_HALED) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 22. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Elongation factor 4 HAMAP-Rule MF_00071
Short name=EF-4 HAMAP-Rule MF_00071
EC=3.6.5.n1 HAMAP-Rule MF_00071

Alternative name(s):
Ribosomal back-translocase LepA HAMAP-Rule MF_00071

Gene names

Name:	lepA HAMAP-Rule MF_00071 EMBL CBV42906.1
Ordered Locus Names:	HELO_3022 EMBL CBV42906.1

Organism

Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9) [Complete proteome] [HAMAP] EMBL CBV42906.1

Taxonomic identifier

768066 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Oceanospirillales › Halomonadaceae › Halomonas ›

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071
Catalytic activity	GTP + H₂O = GDP + phosphate. HAMAP-Rule MF_00071
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily. HAMAP-Rule MF_00071

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00071
Cellular component	Cell inner membrane HAMAP-Rule MF_00071 Cell membrane Membrane
Ligand	GTP-binding RuleBase RU000323 HAMAP-Rule MF_00071 Nucleotide-binding
Molecular function	Hydrolase HAMAP-Rule MF_00071
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC positive regulation of translation Inferred from electronic annotation. Source: HAMAP translational elongation Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP ribosome binding Inferred from electronic annotation. Source: HAMAP translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

23 – 28

GTP By similarity HAMAP-Rule MF_00071

Nucleotide binding

140 – 143

GTP By similarity HAMAP-Rule MF_00071

Sequences

Sequence

Length

Mass (Da)

Tools

E1V4G8 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: 13BBB5B0FD05E12C
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FASTA

605

67,292

        10         20         30         40         50         60 
MTDTDNRKII NGIRNFSIIA HIDHGKSTLA DRLIQTCGGL TERELKEQVL DSMDLERERG 

        70         80         90        100        110        120 
ITIKAQSVTL DYHAQDGNVY QLNFIDTPGH VDFSYEVSRS LYACEGALLV VDAAQGVEAQ 

       130        140        150        160        170        180 
SVANCYTAIE QGLEVLPVLN KMDLPQADPA KVGEEIEEII GLDATDACQV SAKSGLGIDE 

       190        200        210        220        230        240 
LLERLVRDIP PPKGDPEKPL QALIIDSWFD NYLGVVSLVR IFDGTLKKSD KIRISSTGRD 

       250        260        270        280        290        300 
WQATEVGIFT PQRRQTDVLR AGEVGFVVAG IKDIQGAPVG DTITHAKTPD VPRLPGFQKV 

       310        320        330        340        350        360 
KPQVYAGMFP VSSDDYEDFR DALEKLALND ASLDYEPENS DAMGFGFRVG FLGTLHMEIV 

       370        380        390        400        410        420 
QERLEREYDL DLLTTAPTVV YELVMKNGEI RYVSNPSKLP DMADVEEMRE PVVRASILVP 

       430        440        450        460        470        480 
QDFVGNVIAE CEQRRGTQLD MLFLGSQIQL TYELPMSEVV MDFFDRLKSI SKGYASLEYN 

       490        500        510        520        530        540 
FERFEAANLS RLDVLINGDR VDALAVIIHR DHAHSRGRAL VEKMKELIPR QMFDVAIQAA 

       550        560        570        580        590        600 
IGGQVVARST VKALRKNVTA KCYGGDTTRK KKLLEKQKAG KKRMKQVGRV EIPQDAFLAV 


LKVND

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A blueprint of ectoine metabolism from the genome of the Industrial producer Halomonas elongata DSM 2581(T)." Schwibbert K., Marin-Sanguino A., Bagyan I., Heidrich G., Lentzen G., Seitz H., Rampp M., Schuster S.C., Klenk H.-.P., Pfeiffer F., Oesterhelt D., Kunte H.J. Environ. Microbiol. 0:0-0(2010) Cited for: NUCLEOTIDE SEQUENCE. Strain: Type strain: DSM 2581.
[2]	"A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581(T)." Schwibbert K., Marin-Sanguino A., Bagyan I., Heidrich G., Lentzen G., Seitz H., Rampp M., Schuster S.C., Klenk H.P., Pfeiffer F., Oesterhelt D., Kunte H.J. Environ. Microbiol. 13:1973-1994(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN869568 Genomic DNA. Translation: CBV42906.1.
RefSeq	YP_003898091.1. NC_014532.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBV42906; CBV42906; HELO_3022.
GeneID	9747934.
KEGG	hel:HELO_3022.
PATRIC	42355506. VBIHalElo161731_2179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000020624.
KO	K03596.
Enzyme and pathway databases
BioCyc	HELO768066:GJEE-2079-MONOMER.
Family and domain databases
Gene3D	3.30.70.240. 1 hit.
HAMAP	MF_00071. LepA.
InterPro	IPR006297. EF-4. IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR013842. LepA_GTP-bd_C. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF40. PTHR23115:SF40. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF06421. LepA_C. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR01393. lepA. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E1V4G8_HALED

Accession

Primary (citable) accession number: E1V4G8

Entry history

Integrated into UniProtKB/TrEMBL:	November 30, 2010
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information