E1TQ31 (E1TQ31_LACPS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897

Gene names

Name:	gyrA HAMAP-Rule MF_01897 EMBL ADN97231.1
Ordered Locus Names:	LPST_C0006 EMBL ADN97231.1

Organism

Lactobacillus plantarum (strain ST-III) [Complete proteome] [HAMAP]

Taxonomic identifier

889932 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus plantarum › Lactobacillus plantarum subsp. plantarum

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_01897
Ligand	ATP-binding SAAS SAAS013760 HAMAP-Rule MF_01897 DNA-binding SAAS SAAS005743 HAMAP-Rule MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase HAMAP-Rule MF_01897 SAAS SAAS005743
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	125	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

	Sequence		Length	Mass (Da)
	E1TQ31 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: 639F88E067DF40A8	FASTA	853	94,523
	10 20 30 40 50 60 MADSDLSNNR ISDVNLSKTM RTSFLSYAMS VIVARALPDV RDGLKPVHRR ILYGMSELGV 70 80 90 100 110 120 TPDKPYKKSA RIVGDVMGKY HPHGDSAIYE SMVRMAQDFS YRYMLVDGHG NFGSVDGDGA 130 140 150 160 170 180 AAMRYTEARM SKIAVEMLRD INKDTVDWQP NYDDTEREPA VLPARFPNLL VNGATGIAVG 190 200 210 220 230 240 MTTNIPPHNL AEVISAIHLL MDNPDATVAD LMEVLPGPDF PTGGIVMGKS GIRKAYQTGR 250 260 270 280 290 300 GNIIVRAKVD IQDQKNGKQR IIVTELPYMV NKAKLIERIA GLARDKEIEG ITDINDESDR 310 320 330 340 350 360 EGMRIVIDVR RDASASVILN NLYKMTLMQT NFGFNMLAIV KGAPKVLSLK QILIYYLEHQ 370 380 390 400 410 420 EDVIRRRTEF DLKKAQARAH ILEGLRIALD HIDEIIAIIR QSQTSEIAKN QLMDNYGLSD 430 440 450 460 470 480 KQAQAILDMR LVRLTGLERE KIESEYQDLL KAIADYKEIL ASKDRINQII YEELMEIQRK 490 500 510 520 530 540 FGDKRRTELM VGEVLSIEDE DLIEEEEVAV TLTHNGYIKR LPTTEFKSQH RGGRGIQGMD 550 560 570 580 590 600 VHDDDFIEHL LTTSTHDVLL FFTNAGKVYR MKAYEIPEYG RTAKGIPVIN LLGVNSGEKI 610 620 630 640 650 660 QAVVNVTGDA SASDNYLFFT TVKGVVKRTP VQEFANIRSN GLKAITLKDD DELIGVTITD 670 680 690 700 710 720 GHQNVIIGTH DGYAVSFDET TVRSMGRTAS GVRGIRLRDD DFVIGFDVLK PDSNVFIITE 730 740 750 760 770 780 KGYGKQTPAA DYPIKGRGGK GIKTANVTEK NGHLAGLTTV DGQEDIMVMT NQGVMIRFNI 790 800 810 820 830 840 ATVSQTGRAT LGVRLMRLGD DGQVATMAKV DPEPEVDETV ATDATTEAPV DDQTVNADAT 850 TTEPTTDSND SNE

References

[1]	"Complete genome sequence of probiotic Lactobacillus plantarum ST-III." Wang Y., Chen C., Ai L., Zhou F., Zhou Z., Wang L., Zhang H., Guo B., Chen W. Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ST-III EMBL ADN97231.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002222 Genomic DNA. Translation: ADN97231.1.
RefSeq	YP_003923325.1. NC_014554.1.
3D structure databases
ProteinModelPortal	E1TQ31.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADN97231; ADN97231; LPST_C0006.
GeneID	9781948.
KEGG	lps:LPST_C0006.
PATRIC	42496848. VBILacPla173910_0007.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000076278.
KO	K02469.
Enzyme and pathway databases
BioCyc	LPLA889932:GHWQ-51-MONOMER.
Family and domain databases
Gene3D	3.30.1360.40. 1 hit. 3.90.199.10. 2 hits.
HAMAP	MF_01897. GyrA.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E1TQ31_LACPS

Accession

Primary (citable) accession number: E1TQ31

Entry history

Integrated into UniProtKB/TrEMBL:	November 30, 2010
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)