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E1TA04 (E1TA04_BURSG) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate 5-kinase HAMAP-Rule MF_00456
EC=2.7.2.11 HAMAP-Rule MF_00456
Alternative name(s):
Gamma-glutamyl kinase HAMAP-Rule MF_00456
Gene names
Name:proB HAMAP-Rule MF_00456
Ordered Locus Names:BC1003_3032
OrganismBurkholderia sp. (strain CCGE1003) [Complete proteome] [HAMAP] EMBL ADN58981.1
Taxonomic identifier640512 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456 SAAS SAAS011529

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456 SAAS SAAS011529

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456 SAAS SAAS011529

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456 SAAS SAAS011529.

Sequence similarities

Belongs to the glutamate 5-kinase family. HAMAP-Rule MF_00456

Contains 1 PUA domain. HAMAP-Rule MF_00456 SAAS SAAS011529

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain280 – 35879PUA By similarity HAMAP-Rule MF_00456
Nucleotide binding173 – 1742ATP By similarity HAMAP-Rule MF_00456

Sites

Binding site141ATP By similarity HAMAP-Rule MF_00456
Binding site541Substrate By similarity HAMAP-Rule MF_00456
Binding site1411Substrate By similarity HAMAP-Rule MF_00456
Binding site1531Substrate; via amide nitrogen By similarity HAMAP-Rule MF_00456

Sequences

Sequence LengthMass (Da)Tools
E1TA04 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: 578A8480483297B1

FASTA37239,357
        10         20         30         40         50         60 
MRSVIADSRR LVVKVGSSLV TNDGRGLDHA AIGRWAAQIA ALRAQGKEVV LVSSGAIAEG 

        70         80         90        100        110        120 
MQRLGWTKRP REIDELQAAA AVGQMGLAQV YESRFAEHSI QTAQILLTHA DLADRERYLN 

       130        140        150        160        170        180 
ARSTLLTLLR LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLIEGDALI ILTDQQGLFT 

       190        200        210        220        230        240 
ADPRKDPHAT LVQQADAGAP DLEAMAGGAG SSLGRGGMLT KILAAKRAAH SGANTVIASG 

       250        260        270        280        290        300 
READVLSRLA SGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDDGAV EKLTAGGKSL 

       310        320        330        340        350        360 
LPIGIVGVQG AFARGEVIAC LNAAGREVAR GLTNYSSAET KLIQRRPSGE IESVLGYMLE 

       370 
PELIHRDNLV LV

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia sp. CCGE1003."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Daligault H., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G. expand/collapse author list , Martinez-Romero E., Rogel M.A., Auchtung J., Tiedje J.M., Woyke T.
Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCGE1003.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002217 Genomic DNA. Translation: ADN58981.1.
RefSeqYP_003908272.1. NC_014539.1.

3D structure databases

ProteinModelPortalE1TA04.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADN58981; ADN58981; BC1003_3032.
GeneID9767944.
KEGGbgf:BC1003_3032.
PATRIC42212600. VBIBurSp98639_3278.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000246368.
KOK00931.
OMADHLQLRG.

Enzyme and pathway databases

BioCycBSP640512:GBXV-3084-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. Aa_kinase. 1 hit.
SSF88697. PUA-like. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE1TA04_BURSG
AccessionPrimary (citable) accession number: E1TA04
Entry history
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: May 29, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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