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E1SI64 (E1SI64_PANVC) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (uracil(54)-C(5))-methyltransferase HAMAP-Rule MF_01011
EC=2.1.1.35 HAMAP-Rule MF_01011
Alternative name(s):
tRNA(m5U54)-methyltransferase HAMAP-Rule MF_01011
Gene names
Name:trmA HAMAP-Rule MF_01011 EMBL ADO11266.1
Ordered Locus Names:Pvag_3124 EMBL ADO11266.1
OrganismPantoea vagans (strain C9-1) (Pantoea agglomerans (strain C9-1)) [Complete proteome] [HAMAP] EMBL ADO11266.1
Taxonomic identifier712898 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pantoea ›

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNAs By similarity. HAMAP-Rule MF_01011 SAAS SAAS011869

Catalytic activity

S-adenosyl-L-methionine + uridine54 in tRNA = S-adenosyl-L-homocysteine + 5-methyluridine54 in tRNA. HAMAP-Rule MF_01011 SAAS SAAS011869

Sequence similarities

Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily. HAMAP-Rule MF_01011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site3241Nucleophile By similarity HAMAP-Rule MF_01011
Binding site1901S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01011
Binding site2181S-adenosyl-L-methionine; via carbonyl oxygen By similarity HAMAP-Rule MF_01011
Binding site2231S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01011
Binding site2391S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01011
Binding site2991S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01011

Sequences

Sequence LengthMass (Da)Tools
E1SI64 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: 3B500FD4A05E3CA2

FASTA36742,223
        10         20         30         40         50         60 
MTPEHLPIEE YDAQLAEKVS RLETMMTPFA APDVEVFRSP VSHYRMRAEF RLWHDGDDIY 

        70         80         90        100        110        120 
HIIFDQQTRE RIRVDQFPAA SQLINQLMPK MIAAIRDNRT LRHKLFQIDY LSTMSNQIVI 

       130        140        150        160        170        180 
SLLYHRKLDD EWLQAAAALR DQLRAEGLDV QLIGRATKTK ICLDRDFVDE CLPVAGREII 

       190        200        210        220        230        240 
YRQVENSFTQ PNAAMNIQML EWALDVTQDS QGDLLELYCG NGNFSLALAR NFRRVLATEI 

       250        260        270        280        290        300 
AKPSVASAQY NIAVNQIDNV QIIRMAAEEF TQAMNGVRSF NRLEGIDLTS YECETIFVDP 

       310        320        330        340        350        360 
PRSGLDEETV KMVQAYPRIL YISCNPQTLS DNLQTLSETH EVTRLALFDQ FPYTHHMECG 


VLLTRKA

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References

[1]"Genome sequence of the biocontrol agent Pantoea vagans strain C9-1."
Smits T.H., Rezzonico F., Kamber T., Goesmann A., Ishimaru C.A., Stockwell V.O., Frey J.E., Duffy B.
J. Bacteriol. 192:6486-6487(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C9-1 EMBL ADO11266.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002206 Genomic DNA. Translation: ADO11266.1.
RefSeqYP_003932715.1. NC_014562.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADO11266; ADO11266; Pvag_3124.
GeneID9791636.
KEGGpva:Pvag_3124.
PATRIC42422419. VBIPanVag152020_3284.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000218626.
KOK00557.

Enzyme and pathway databases

BioCycPVAG712898:GHQ2-3345-MONOMER.

Family and domain databases

HAMAPMF_01011. tRNA_methyltr_TrmA.
InterProIPR011869. TrmA_MeTrfase_proteobac.
IPR010280. U5_MeTrfase.
[Graphical view]
PfamPF05958. tRNA_U5-meth_tr. 1 hit.
[Graphical view]
TIGRFAMsTIGR02143. trmA_only. 1 hit.
PROSITEPS01230. TRMA_1. 1 hit.
PS01231. TRMA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE1SI64_PANVC
AccessionPrimary (citable) accession number: E1SI64
Entry history
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: May 1, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info