Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Methanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847) (Methanoplanus petrolearius)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathway: chorismate biosynthesis

This protein is involved in the pathway chorismate biosynthesis, which is part of Metabolic intermediate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271Shikimate-3-phosphateUniRule annotation
Binding sitei122 – 1221PhosphoenolpyruvateUniRule annotation
Binding sitei195 – 1951Shikimate-3-phosphateUniRule annotation
Active sitei310 – 3101Proton acceptorUniRule annotation
Binding sitei337 – 3371Shikimate-3-phosphateUniRule annotation
Active sitei338 – 3381Proton donorUniRule annotation
Binding sitei341 – 3411PhosphoenolpyruvateUniRule annotation
Binding sitei384 – 3841PhosphoenolpyruvateUniRule annotation
Binding sitei409 – 4091PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationImported

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesisUniRule annotation

Enzyme and pathway databases

BioCyciMPET679926:GHOF-2759-MONOMER.
UniPathwayiUPA00053.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:Mpet_2701Imported
OrganismiMethanolacinia petrolearia (strain DSM 11571 / OCM 486 / SEBR 4847) (Methanoplanus petrolearius)Imported
Taxonomic identifieri679926 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanomicrobiaceaeMethanolacinia
ProteomesiUP000006565 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi679926.Mpet_2701.

Structurei

3D structure databases

ProteinModelPortaliE1RGD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232Shikimate-3-phosphate bindingUniRule annotation
Regioni92 – 954PhosphoenolpyruvateUniRule annotation
Regioni167 – 1693Shikimate-3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000247372.
KOiK00800.
OMAiHDHRLVM.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.

Sequencei

Sequence statusi: Complete.

E1RGD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGIVINRHR DVDLTFSAPP SKSYTHRALF CAALADGISI IENPLYSGDT
60 70 80 90 100
EVTCRALGQL GVRTERTADG IIVDGCGGVM PPGGDVTIDC EGSGTSLRFL
110 120 130 140 150
ATFALLYPGR VTLTGSPRMK ERPVGELTGA LREIGGDIRF TERPGFPPVE
160 170 180 190 200
ISGELSGGSV SIDASKSSQF ISSMLIPAPY AEDPVDIIPE GHVASESYLD
210 220 230 240 250
ITVDVMEKFG AAVERMPDGG FHVPSGAYVP RDYRVEGDYS SASYLFAIAA
260 270 280 290 300
ICGGTVTVNN LNPDSVQGDT AFAGALASMG CRVTKDAGSV TVTRKGPLSG
310 320 330 340 350
VDIDMSSSPD TVQTLAAVAV FADSPTRITG VSHLIYKESD RIGAIGRMVE
360 370 380 390 400
GCGAGFEYND NEGEIVITPG KIHGFVLDPV DDHRTAMSGA VIGLGAGGVT
410 420
IKDPECVGKS YPGFWDELRR AGL
Length:423
Mass (Da):44,295
Last modified:November 30, 2010 - v1
Checksum:iA030EFCE2FDFB4AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002117 Genomic DNA. Translation: ADN37444.1.
RefSeqiWP_013330617.1. NC_014507.1.
YP_003895882.1. NC_014507.1.

Genome annotation databases

EnsemblBacteriaiADN37444; ADN37444; Mpet_2701.
GeneIDi9745196.
KEGGimpi:Mpet_2701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002117 Genomic DNA. Translation: ADN37444.1.
RefSeqiWP_013330617.1. NC_014507.1.
YP_003895882.1. NC_014507.1.

3D structure databases

ProteinModelPortaliE1RGD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi679926.Mpet_2701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADN37444; ADN37444; Mpet_2701.
GeneIDi9745196.
KEGGimpi:Mpet_2701.

Phylogenomic databases

HOGENOMiHOG000247372.
KOiK00800.
OMAiHDHRLVM.

Enzyme and pathway databases

UniPathwayiUPA00053.
BioCyciMPET679926:GHOF-2759-MONOMER.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 11571 / OCM 486 / SEBR 4847Imported.

Entry informationi

Entry nameiE1RGD0_METP4
AccessioniPrimary (citable) accession number: E1RGD0
Entry historyi
Integrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: June 24, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.