E1R573 (E1R573_SPISS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase HAMAP-Rule MF_00126
EC=6.1.1.18 HAMAP-Rule MF_00126

Alternative name(s):
Glutaminyl-tRNA synthetase HAMAP-Rule MF_00126

Gene names

Name:	glnS HAMAP-Rule MF_00126
Ordered Locus Names:	Spirs_1481

Organism

Spirochaeta smaragdinae (strain DSM 11293 / JCM 15392 / SEBR 4228) [Complete proteome] [HAMAP]

Taxonomic identifier

573413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Spirochaetaceae › Spirochaeta › Spirochaeta smaragdinae

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126 SAAS SAAS020059
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00126 SAAS SAAS020059
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00126 SAAS SAAS020059.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00126 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00126 RuleBase RU003489 SAAS SAAS004514
Cellular component	Cytoplasm HAMAP-Rule MF_00126 SAAS SAAS020059
Ligand	ATP-binding SAAS SAAS004514 HAMAP-Rule MF_00126 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00126 SAAS SAAS004514 RuleBase RU003489 EMBL ADK80608.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	34 – 44	11	"HIGH" region By similarity HAMAP-Rule MF_00126
	Motif	268 – 272	5	"KMSKS" region By similarity HAMAP-Rule MF_00126
Sites
	Binding site	271	1	ATP By similarity HAMAP-Rule MF_00126

Sequences

	Sequence		Length	Mass (Da)
	E1R573 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: 64EE2ED90EC8E409	FASTA	560	64,472
	10 20 30 40 50 60 MADAEKSSLN FIERIIEDDF ARGKHSQVVT RFPPEPNGYL HIGHAKSICL NFGLAKKYGG 70 80 90 100 110 120 RCHLRFDDTN PEKESLEYIE SIKRDVQWLG FSWGAHEYYA SDYFEELYKR AVRLIKAGKA 130 140 150 160 170 180 YVDDLSPEQM REYRGTLTKP GKESPNRTRS IEENLDLFER MRKGEFPDGS MLLRAKIDMA 190 200 210 220 230 240 SGNMNMRDPA LYRIRRINHH RTGDAWCIYP MYDFTHPLSD AIEGITHSIC TLEFEDHRPL 250 260 270 280 290 300 YDWSVEETEM ECHPRQIEFA RLNLTYTVMS KRILLQLVTE GHVSGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYTPEAIRQF ADIIGVAKVN STVDMALLEH CLREDLNLRA LRVMVVLDPV KVVIDNYPEG 370 380 390 400 410 420 KEELLEAENN PEDPDGGMRM VPFSREVYIE REDFMEDPPK KFFRLAPGRE IRLKHAYYIT 430 440 450 460 470 480 CVSVEKNEAG EITTIHCSYD PESRGGGTPD GRRVKGTSHW VSVSHAKELE VRLYDNLFTS 490 500 510 520 530 540 PEPGSRTGNF LDDLNPDSLS TIKAMAEPSL IDAEPEAHYQ FLRKGYFTVD PKESKKGAPV 550 560 FNRTVTLRDS WAKLQKKIEA

References

[1]

"Complete genome sequence of Spirochaeta smaragdinae type strain (SEBR 4228)." Mavromatis K., Yasawong M., Chertkov O., Lapidus A., Lucas S., Nolan M., Glavina Del Rio T., Tice H., Cheng J., Pitluck S., Liolios K., Ivanova N., Tapia R., Han C., Bruce D., Goodwin L., Pati A., Chen A. , Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Detter J., Rohde M., Brambilla E., Spring S., Goker M., Sikorski J., Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P., Klenk H., Kyrpides N. Stand. Genomic Sci. 3:136-144(2010) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11293 / JCM 15392 / SEBR 4228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002116 Genomic DNA. Translation: ADK80608.1.
RefSeq	YP_003803202.1. NC_014364.1.
3D structure databases
ProteinModelPortal	E1R573.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADK80608; ADK80608; Spirs_1481.
GeneID	9489570.
KEGG	ssm:Spirs_1481.
PATRIC	42526916. VBISpiSma89994_1526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
Enzyme and pathway databases
BioCyc	SSMA573413:GI0C-1498-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E1R573_SPISS

Accession

Primary (citable) accession number: E1R573

Entry history

Integrated into UniProtKB/TrEMBL:	November 30, 2010
Last sequence update:	November 30, 2010
Last modified:	May 29, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)