E1R3Z4 (E1R3Z4_SPISS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase HAMAP-Rule MF_01405
EC=3.6.1.19 HAMAP-Rule MF_01405

Alternative name(s):
Non-standard purine NTP pyrophosphatase HAMAP-Rule MF_01405
Nucleoside-triphosphate diphosphatase HAMAP-Rule MF_01405
Nucleoside-triphosphate pyrophosphatase HAMAP-Rule MF_01405

Gene names

Ordered Locus Names:

Spirs_1289

Organism

Spirochaeta smaragdinae (strain DSM 11293 / JCM 15392 / SEBR 4228) [Complete proteome] [HAMAP]

Taxonomic identifier

573413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Spirochaetaceae › Spirochaeta › Spirochaeta smaragdinae

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP-Rule MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP-Rule MF_01405 SAAS SAAS020922
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity. HAMAP-Rule MF_01405 SAAS SAAS020922
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01405 SAAS SAAS020922
Sequence similarities	Belongs to the HAM1 NTPase family. HAMAP-Rule MF_01405 RuleBase RU003781

Ontologies

Keywords
Biological process	Nucleotide metabolism HAMAP-Rule MF_01405 SAAS SAAS020922
Ligand	Magnesium HAMAP-Rule MF_01405 SAAS SAAS020922 Manganese HAMAP-Rule MF_01405 SAAS SAAS020922 Metal-binding HAMAP-Rule MF_01405 SAAS SAAS020922 Nucleotide-binding HAMAP-Rule MF_01405 SAAS SAAS020922
Molecular function	Hydrolase HAMAP-Rule MF_01405 RuleBase RU003781 SAAS SAAS020922 EMBL ADK80416.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleoside triphosphate catabolic process Inferred from electronic annotation. Source: InterPro purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	7 – 12	6	Substrate binding By similarity HAMAP-Rule MF_01405
	Region	67 – 68	2	Substrate binding By similarity HAMAP-Rule MF_01405
Sites
	Metal binding	38	1	Magnesium or manganese By similarity HAMAP-Rule MF_01405
	Metal binding	67	1	Magnesium or manganese By similarity HAMAP-Rule MF_01405
	Binding site	155	1	Substrate By similarity HAMAP-Rule MF_01405
	Binding site	175	1	Substrate By similarity HAMAP-Rule MF_01405
	Binding site	181	1	Substrate By similarity HAMAP-Rule MF_01405

Sequences

	Sequence		Length	Mass (Da)
	E1R3Z4 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: 6084A513A2695B93	FASTA	199	21,886
	10 20 30 40 50 60 MNMFVATNNM HKVRELSAIL TGWELKLPAE AGIRFEHEEI GSSFMENALG KAQALFTQLK 70 80 90 100 110 120 KPVIADDSGL VVPALGGEPG IYSARYGSRP GEANMEAAER NNYLLHKMQG VQERRGFFVC 130 140 150 160 170 180 CMALIVGEER IFTVQEIFPG EIAEAPSGEG GFGYDPIFFL PEYGKTAAQL SDDEKNRISH 190 RGRAGMRMAA ILDSLRNNA

References

[1]

"Complete genome sequence of Spirochaeta smaragdinae type strain (SEBR 4228)." Mavromatis K., Yasawong M., Chertkov O., Lapidus A., Lucas S., Nolan M., Glavina Del Rio T., Tice H., Cheng J., Pitluck S., Liolios K., Ivanova N., Tapia R., Han C., Bruce D., Goodwin L., Pati A., Chen A. , Palaniappan K., Land M., Hauser L., Chang Y., Jeffries C., Detter J., Rohde M., Brambilla E., Spring S., Goker M., Sikorski J., Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P., Klenk H., Kyrpides N. Stand. Genomic Sci. 3:136-144(2010) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 11293 / JCM 15392 / SEBR 4228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002116 Genomic DNA. Translation: ADK80416.1.
RefSeq	YP_003803010.1. NC_014364.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADK80416; ADK80416; Spirs_1289.
GeneID	9489373.
KEGG	ssm:Spirs_1289.
PATRIC	42526518. VBISpiSma89994_1332.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000293319.
KO	K02428.
OMA	DGTLVWP.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase.
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
PANTHER	PTHR11067. PTHR11067. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E1R3Z4_SPISS

Accession

Primary (citable) accession number: E1R3Z4

Entry history

Integrated into UniProtKB/TrEMBL:	November 30, 2010
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)