E1QCT1 (RNC_MYCPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	MPNE_0641

Organism

Mycoplasma pneumoniae (strain ATCC 15531 / NBRC 14401 / NCTC 10119 / FH) [Complete proteome] [HAMAP]

Taxonomic identifier

722438 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000416609
Regions
	Domain	18 – 141	124	RNase III
Sites
	Active site	63	1	Potential
	Active site	130	1	By similarity
	Metal binding	59	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Metal binding	130	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	E1QCT1 [UniParc]. Last modified November 30, 2010. Version 1. Checksum: 47C2BC66F7450D8C	FASTA	282	32,653
	10 20 30 40 50 60 MKNKKDKTQK PKVIDEKFVA FFKSLNIEPQ NWQFYEDAFV HSSYVNENED ARASYDRLEF 70 80 90 100 110 120 LGDALIDFIV AKKLFELYPN YNEGMLTRTK IEIVKGENLN RIGKELNFGN FIKLGKGMPY 130 140 150 160 170 180 TETLFGDVLE ALVAAIYEDL GIEKANQFVE EHIFKKTYSE ILKYNFFSLF QEQKLPEPRV 190 200 210 220 230 240 RVSLTSNNLV LSIIELNGDI IWSQAVPNSK HYDDKSVLEH NAMSAFTQFL KSGKGINFFS 250 260 270 280 DIKNKLDSQK PMRALTVRPK KINWKARKPK LKALKNKVKA DS

References

[1]

"Targeted chromosomal knockouts in Mycoplasma pneumoniae." Krishnakumar R., Assad-Garcia N., Benders G.A., Phan Q., Montague M.G., Glass J.I. Appl. Environ. Microbiol. 76:5297-5299(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15531 / NBRC 14401 / NCTC 10119 / FH.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002077 Genomic DNA. Translation: ADK87079.1.
RefSeq	YP_005881546.1. NC_017504.1.
3D structure databases
ProteinModelPortal	E1QCT1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADK87079; ADK87079; MPNE_0641.
GeneID	12397561.
KEGG	mpj:MPNE_0641.
PATRIC	43132233. VBIMycPne158725_0619.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000154168.
KO	K03685.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_MYCPB

Accession

Primary (citable) accession number: E1QCT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2012
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program