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E1QCT1 (RNC_MYCPB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:MPNE_0641
OrganismMycoplasma pneumoniae (strain ATCC 15531 / NBRC 14401 / NCTC 10119 / FH) [Complete proteome] [HAMAP]
Taxonomic identifier722438 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 1 RNase III domain.

Ontologies

Keywords
   Biological processmRNA processing
rRNA processing
tRNA processing
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA catabolic process

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonuclease III activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000416609

Regions

Domain18 – 141124RNase III

Sites

Active site631 Potential
Active site1301 By similarity
Metal binding591Magnesium By similarity
Metal binding1271Magnesium By similarity
Metal binding1301Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
E1QCT1 [UniParc].

Last modified November 30, 2010. Version 1.
Checksum: 47C2BC66F7450D8C

FASTA28232,653
        10         20         30         40         50         60 
MKNKKDKTQK PKVIDEKFVA FFKSLNIEPQ NWQFYEDAFV HSSYVNENED ARASYDRLEF 

        70         80         90        100        110        120 
LGDALIDFIV AKKLFELYPN YNEGMLTRTK IEIVKGENLN RIGKELNFGN FIKLGKGMPY 

       130        140        150        160        170        180 
TETLFGDVLE ALVAAIYEDL GIEKANQFVE EHIFKKTYSE ILKYNFFSLF QEQKLPEPRV 

       190        200        210        220        230        240 
RVSLTSNNLV LSIIELNGDI IWSQAVPNSK HYDDKSVLEH NAMSAFTQFL KSGKGINFFS 

       250        260        270        280 
DIKNKLDSQK PMRALTVRPK KINWKARKPK LKALKNKVKA DS 

« Hide

References

[1]"Targeted chromosomal knockouts in Mycoplasma pneumoniae."
Krishnakumar R., Assad-Garcia N., Benders G.A., Phan Q., Montague M.G., Glass J.I.
Appl. Environ. Microbiol. 76:5297-5299(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15531 / NBRC 14401 / NCTC 10119 / FH.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002077 Genomic DNA. Translation: ADK87079.1.
RefSeqYP_005881546.1. NC_017504.1.

3D structure databases

ProteinModelPortalE1QCT1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADK87079; ADK87079; MPNE_0641.
GeneID12397561.
KEGGmpj:MPNE_0641.
PATRIC43132233. VBIMycPne158725_0619.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000154168.
KOK03685.

Enzyme and pathway databases

BioCycMPNE722438:GLHK-641-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTSM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_MYCPB
AccessionPrimary (citable) accession number: E1QCT1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 30, 2010
Last modified: April 16, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families