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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Helicobacter pylori (strain PeCan4)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotationSAAS annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (HPPC_07185)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (HPPC_07185)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52NADPUniRule annotation1
Binding sitei54NADPUniRule annotation1
Active sitei109UniRule annotation1
Binding sitei135NADP; via amide nitrogenUniRule annotation1
Metal bindingi193Magnesium 1UniRule annotation1
Metal bindingi193Magnesium 2UniRule annotation1
Metal bindingi197Magnesium 1UniRule annotation1
Metal bindingi229Magnesium 2UniRule annotation1
Metal bindingi233Magnesium 2UniRule annotation1
Binding sitei254SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 29NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseImported, OxidoreductaseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciHPYL765963:G1GNG-334-MONOMER
UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:HPPC_01675Imported
OrganismiHelicobacter pylori (strain PeCan4)Imported
Taxonomic identifieri765963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000006863 Componenti: Chromosome

Structurei

3D structure databases

ProteinModelPortaliE1Q589
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 184KARI N-terminal RossmannInterPro annotationAdd BLAST182
Domaini185 – 329KARI C-terminal knottedInterPro annotationAdd BLAST145

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000016230
KOiK00053
OMAiRAMFSWL
OrthoDBiPOG091H063Y

Family and domain databases

HAMAPiMF_00435 IlvC, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013023 KARI
IPR000506 KARI_C
IPR013116 KARI_N
IPR014359 KARI_prok
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 1 hit
PF07991 IlvN, 1 hit
PIRSFiPIRSF000116 IlvC_gammaproteo, 2 hits
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 1 hit
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

E1Q589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPVYYDKD IDLGVIQSLQ VGIIGYGAQG EAQALNLRDS KVKVRIGLYQ
60 70 80 90 100
GSLSVSKAKA EGFEVLEVKE LVQQSDVIMA LLPDELHKEV LEKEVIPFLK
110 120 130 140 150
EGQIVGFAHG FSVHFNQVVL PKGVGAILVA PKGPGSALRE EYLKNRGLYH
160 170 180 190 200
LIAIEQESSK NNAKAVALSY AKAMGGGRMG VLETSFKEEC ESDLFGEQAV
210 220 230 240 250
LCGGLEAIVR MGFETLIKAG YPEELAYFEC VHEVKLVADL LHYKGVEGLR
260 270 280 290 300
KHISNTAEFG AIKAREPMGN LLEKRMQKIL KKIQNGAFAK DFLLEKSLNY
310 320 330
PRLNTERKAL KETKIEQIGE ILRVPFNHKK
Length:330
Mass (Da):36,509
Last modified:November 30, 2010 - v1
Checksum:i793FCE7631E58AD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002074 Genomic DNA Translation: ADO06580.1
RefSeqiWP_001207749.1, NC_014555.1

Genome annotation databases

EnsemblBacteriaiADO06580; ADO06580; HPPC_01675
KEGGihpc:HPPC_01675

Similar proteinsi

Entry informationi

Entry nameiE1Q589_HELPP
AccessioniPrimary (citable) accession number: E1Q589
Entry historyiIntegrated into UniProtKB/TrEMBL: November 30, 2010
Last sequence update: November 30, 2010
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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