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Protein

DNA polymerase I

Gene

polA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58CalciumCombined sources1
Metal bindingi60CalciumCombined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, DNA-directed DNA polymeraseUniRule annotation, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repairUniRule annotation, DNA replicationUniRule annotation
LigandCalciumCombined sources, Metal-bindingCombined sources

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IUniRule annotation (EC:2.7.7.7UniRule annotation)
Gene namesi
Name:polAUniRule annotation
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.UniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XO7X-ray2.85A1-580[»]
2XY5X-ray2.22A1-580[»]
2XY6X-ray2.30A1-580[»]
2XY7X-ray3.05A1-580[»]
2Y1IX-ray2.78A1-580[»]
2Y1JX-ray2.15A1-580[»]
4B9LX-ray2.05A1-580[»]
4B9MX-ray2.05A1-580[»]
4B9NX-ray2.20A1-580[»]
4B9SX-ray1.73A1-580[»]
4B9TX-ray2.65A1-580[»]
4B9UX-ray2.10A1-580[»]
4B9VX-ray2.00A1-580[»]
4DSIX-ray2.05A2-580[»]
4DSJX-ray2.86A/B2-580[»]
4DSKX-ray2.18A2-580[»]
4DSLX-ray2.45A2-580[»]
4DWIX-ray1.85A2-580[»]
4UQGX-ray2.00A2-580[»]
4YFUX-ray1.50A/D2-580[»]
ProteinModelPortaliE1C9K5.
SMRiE1C9K5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiE1C9K5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 1703'-5' exonucleaseInterPro annotationAdd BLAST167
Domaini337 – 544POLAcInterPro annotationAdd BLAST208

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.UniRule annotation

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiView protein in InterPro
IPR002562. 3'-5'_exonuclease_dom.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
PfamiView protein in Pfam
PF00476. DNA_pol_A. 1 hit.
PRINTSiPR00868. DNAPOLI.
SMARTiView protein in SMART
SM00474. 35EXOc. 1 hit.
SM00482. POLAc. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiView protein in PROSITE
PS00447. DNA_POLYMERASE_A. 1 hit.

Sequencei

Sequence statusi: Complete.

E1C9K5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AKMAFTLADR VTEEMLADKA ALVVEVVEEN YHDAPIVGIA VVNEHGRFFL
60 70 80 90 100
RPETALADPQ FVAWLGDETK KKSMFDSKRA AVALKWKGIE LCGVSFDLLL
110 120 130 140 150
AAYLLDPAQG VDDVAAAAKM KQYEAVRPDE AVYGKGAKRA VPDEPVLAEH
160 170 180 190 200
LVRKAAAIWA LERPFLDELR RNEQDRLLVE LEQPLSSILA EMEFAGVKVD
210 220 230 240 250
TKRLEQMGEE LAEQLRTVEQ RIYELAGQEF NINSPKQLGV ILFEKLQLPV
260 270 280 290 300
LKKSKTGYST SADVLEKLAP YHEIVENILH YRQLGKLQST YIEGLLKVVR
310 320 330 340 350
PDTKKVHTIF NQALTQTGRL SSTEPNLQNI PIRLEEGRKI RQAFVPSESD
360 370 380 390 400
WLIFAADYSQ IELRVLAHIA EDDNLMEAFR RDLDIHTKTA MDIFQVSEDE
410 420 430 440 450
VTPNMRRQAK AVNFGIVYGI SDYGLAQNLN ISRKEAAEFI ERYFESFPGV
460 470 480 490 500
KRYMENIVQE AKQKGYVTTL LHRRRYLPDI TSRNFNVRSF AERMAMNTPI
510 520 530 540 550
QGSAADIIKK AMIDLNARLK EERLQARLLL QVHDELILEA PKEEMERLCR
560 570 580
LVPEVMEQAV TLRVPLKVDY HYGSTWYDAK
Length:580
Mass (Da):66,131
Last modified:November 2, 2010 - v1
Checksum:i355FFB1153F1B829
GO

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiE1C9K5_GEOSE
AccessioniPrimary (citable) accession number: E1C9K5
Entry historyiIntegrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: July 5, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources