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E1C9K5

- E1C9K5_GEOSE

UniProt

E1C9K5 - E1C9K5_GEOSE

Protein

DNA polymerase

Gene
N/A
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi58 – 581Calcium
    Metal bindingi60 – 601Calcium
    Metal bindingi357 – 3571MagnesiumImported
    Metal bindingi358 – 3581Magnesium; via carbonyl oxygenImported
    Metal bindingi534 – 5341MagnesiumImported

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: InterPro
    2. DNA binding Source: UniProtKB-KW
    3. DNA-directed DNA polymerase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    NucleotidyltransferaseUniRule annotation, Transferase

    Keywords - Biological processi

    DNA replicationUniRule annotation

    Keywords - Ligandi

    CalciumImported, DNA-bindingUniRule annotation, MagnesiumImported, Metal-bindingImported

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymeraseUniRule annotation (EC:2.7.7.7UniRule annotation)
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XO7X-ray2.85A1-580[»]
    2XY5X-ray2.22A1-580[»]
    2XY6X-ray2.30A1-580[»]
    2XY7X-ray3.05A1-580[»]
    2Y1IX-ray2.78A1-580[»]
    2Y1JX-ray2.15A1-580[»]
    4B9LX-ray2.05A1-580[»]
    4B9MX-ray2.05A1-580[»]
    4B9NX-ray2.20A1-580[»]
    4B9SX-ray1.73A1-580[»]
    4B9TX-ray2.65A1-580[»]
    4B9UX-ray2.10A1-580[»]
    4B9VX-ray2.00A1-580[»]
    4DSIX-ray2.05A2-580[»]
    4DSJX-ray2.86A/B2-580[»]
    4DSKX-ray2.18A2-580[»]
    4DSLX-ray2.45A2-580[»]
    4DWIX-ray1.85A2-580[»]
    ProteinModelPortaliE1C9K5.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiE1C9K5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 134Sulfate 3 binding
    Regioni234 – 2374Sulfate 8 binding

    Sequence similaritiesi

    Belongs to the DNA polymerase type-A family.UniRule annotation

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    InterProiIPR002562. 3'-5'_exonuclease_dom.
    IPR019760. DNA-dir_DNA_pol_A_CS.
    IPR001098. DNA-dir_DNA_pol_A_palm_dom.
    IPR018320. DNA_polymerase_1.
    IPR002298. DNA_polymerase_A.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00476. DNA_pol_A. 1 hit.
    [Graphical view]
    PRINTSiPR00868. DNAPOLI.
    SMARTiSM00474. 35EXOc. 1 hit.
    SM00482. POLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00593. pola. 1 hit.
    PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E1C9K5-1 [UniParc]FASTAAdd to Basket

    « Hide

    AKMAFTLADR VTEEMLADKA ALVVEVVEEN YHDAPIVGIA VVNEHGRFFL    50
    RPETALADPQ FVAWLGDETK KKSMFDSKRA AVALKWKGIE LCGVSFDLLL 100
    AAYLLDPAQG VDDVAAAAKM KQYEAVRPDE AVYGKGAKRA VPDEPVLAEH 150
    LVRKAAAIWA LERPFLDELR RNEQDRLLVE LEQPLSSILA EMEFAGVKVD 200
    TKRLEQMGEE LAEQLRTVEQ RIYELAGQEF NINSPKQLGV ILFEKLQLPV 250
    LKKSKTGYST SADVLEKLAP YHEIVENILH YRQLGKLQST YIEGLLKVVR 300
    PDTKKVHTIF NQALTQTGRL SSTEPNLQNI PIRLEEGRKI RQAFVPSESD 350
    WLIFAADYSQ IELRVLAHIA EDDNLMEAFR RDLDIHTKTA MDIFQVSEDE 400
    VTPNMRRQAK AVNFGIVYGI SDYGLAQNLN ISRKEAAEFI ERYFESFPGV 450
    KRYMENIVQE AKQKGYVTTL LHRRRYLPDI TSRNFNVRSF AERMAMNTPI 500
    QGSAADIIKK AMIDLNARLK EERLQARLLL QVHDELILEA PKEEMERLCR 550
    LVPEVMEQAV TLRVPLKVDY HYGSTWYDAK 580
    Length:580
    Mass (Da):66,131
    Last modified:November 2, 2010 - v1
    Checksum:i355FFB1153F1B829
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XO7 X-ray 2.85 A 1-580 [» ]
    2XY5 X-ray 2.22 A 1-580 [» ]
    2XY6 X-ray 2.30 A 1-580 [» ]
    2XY7 X-ray 3.05 A 1-580 [» ]
    2Y1I X-ray 2.78 A 1-580 [» ]
    2Y1J X-ray 2.15 A 1-580 [» ]
    4B9L X-ray 2.05 A 1-580 [» ]
    4B9M X-ray 2.05 A 1-580 [» ]
    4B9N X-ray 2.20 A 1-580 [» ]
    4B9S X-ray 1.73 A 1-580 [» ]
    4B9T X-ray 2.65 A 1-580 [» ]
    4B9U X-ray 2.10 A 1-580 [» ]
    4B9V X-ray 2.00 A 1-580 [» ]
    4DSI X-ray 2.05 A 2-580 [» ]
    4DSJ X-ray 2.86 A/B 2-580 [» ]
    4DSK X-ray 2.18 A 2-580 [» ]
    4DSL X-ray 2.45 A 2-580 [» ]
    4DWI X-ray 1.85 A 2-580 [» ]
    ProteinModelPortali E1C9K5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei E1C9K5.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    InterProi IPR002562. 3'-5'_exonuclease_dom.
    IPR019760. DNA-dir_DNA_pol_A_CS.
    IPR001098. DNA-dir_DNA_pol_A_palm_dom.
    IPR018320. DNA_polymerase_1.
    IPR002298. DNA_polymerase_A.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00476. DNA_pol_A. 1 hit.
    [Graphical view ]
    PRINTSi PR00868. DNAPOLI.
    SMARTi SM00474. 35EXOc. 1 hit.
    SM00482. POLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00593. pola. 1 hit.
    PROSITEi PS00447. DNA_POLYMERASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Chemical discrimination between dC and 5MedC via their hydroxylamine adducts."
      Munzel M., Lercher L., Muller M., Carell T.
      Nucleic Acids Res. 38:e192-e192(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS).
    2. "Crystal structures and repair studies reveal the identity and the base-pairing properties of the UV-induced spore photoproduct DNA lesion."
      Heil K., Kneuttinger A.C., Schneider S., Lischke U., Carell T.
      Chemistry 17:9651-9657(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
    3. "Reversible bond formation enables the replication and amplification of a crosslinking salen complex as an orthogonal base pair."
      Kaul C., Muller M., Wagner M., Schneider S., Carell T.
      Nat. Chem. 3:794-800(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS).
    4. "Biochemical and structural insights into the fidelity of bacillus stearothermophilus DNA polymerase."
      Gan J.H., Abdur R., Liu H.H., Sheng J., Caton-Willians J., Soares A.S., Huang Z.
      Submitted (FEB-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.86 ANGSTROMS) OF 2-580.
    5. "Unexpected non-Hoogsteen-based mutagenicity mechanism of FaPy-DNA lesions."
      Gehrke T.H., Lischke U., Gasteiger K.L., Schneider S., Arnold S., Muller H.C., Stephenson D.S., Zipse H., Carell T.
      Nat. Chem. Biol. 9:455-461(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

    Entry informationi

    Entry nameiE1C9K5_GEOSE
    AccessioniPrimary (citable) accession number: E1C9K5
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 2, 2010
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3