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E1C9K5

- E1C9K5_GEOSE

UniProt

E1C9K5 - E1C9K5_GEOSE

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Protein

DNA polymerase

Gene
N/A
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi357 – 3571MagnesiumImported
Metal bindingi358 – 3581Magnesium; via carbonyl oxygenImported
Metal bindingi534 – 5341MagnesiumImported

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: UniProtKB-KW
  3. DNA-directed DNA polymerase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA-dependent DNA replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

NucleotidyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

DNA replicationUniRule annotation

Keywords - Ligandi

CalciumImported, DNA-bindingUniRule annotation, MagnesiumImported, Metal-bindingImported

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymeraseUniRule annotation (EC:2.7.7.7UniRule annotation)
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XO7X-ray2.85A1-580[»]
2XY5X-ray2.22A1-580[»]
2XY6X-ray2.30A1-580[»]
2XY7X-ray3.05A1-580[»]
2Y1IX-ray2.78A1-580[»]
2Y1JX-ray2.15A1-580[»]
4B9LX-ray2.05A1-580[»]
4B9MX-ray2.05A1-580[»]
4B9NX-ray2.20A1-580[»]
4B9SX-ray1.73A1-580[»]
4B9TX-ray2.65A1-580[»]
4B9UX-ray2.10A1-580[»]
4B9VX-ray2.00A1-580[»]
4DSIX-ray2.05A2-580[»]
4DSJX-ray2.86A/B2-580[»]
4DSKX-ray2.18A2-580[»]
4DSLX-ray2.45A2-580[»]
4DWIX-ray1.85A2-580[»]
ProteinModelPortaliE1C9K5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiE1C9K5.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.UniRule annotation

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E1C9K5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AKMAFTLADR VTEEMLADKA ALVVEVVEEN YHDAPIVGIA VVNEHGRFFL
60 70 80 90 100
RPETALADPQ FVAWLGDETK KKSMFDSKRA AVALKWKGIE LCGVSFDLLL
110 120 130 140 150
AAYLLDPAQG VDDVAAAAKM KQYEAVRPDE AVYGKGAKRA VPDEPVLAEH
160 170 180 190 200
LVRKAAAIWA LERPFLDELR RNEQDRLLVE LEQPLSSILA EMEFAGVKVD
210 220 230 240 250
TKRLEQMGEE LAEQLRTVEQ RIYELAGQEF NINSPKQLGV ILFEKLQLPV
260 270 280 290 300
LKKSKTGYST SADVLEKLAP YHEIVENILH YRQLGKLQST YIEGLLKVVR
310 320 330 340 350
PDTKKVHTIF NQALTQTGRL SSTEPNLQNI PIRLEEGRKI RQAFVPSESD
360 370 380 390 400
WLIFAADYSQ IELRVLAHIA EDDNLMEAFR RDLDIHTKTA MDIFQVSEDE
410 420 430 440 450
VTPNMRRQAK AVNFGIVYGI SDYGLAQNLN ISRKEAAEFI ERYFESFPGV
460 470 480 490 500
KRYMENIVQE AKQKGYVTTL LHRRRYLPDI TSRNFNVRSF AERMAMNTPI
510 520 530 540 550
QGSAADIIKK AMIDLNARLK EERLQARLLL QVHDELILEA PKEEMERLCR
560 570 580
LVPEVMEQAV TLRVPLKVDY HYGSTWYDAK
Length:580
Mass (Da):66,131
Last modified:November 2, 2010 - v1
Checksum:i355FFB1153F1B829
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2XO7 X-ray 2.85 A 1-580 [» ]
2XY5 X-ray 2.22 A 1-580 [» ]
2XY6 X-ray 2.30 A 1-580 [» ]
2XY7 X-ray 3.05 A 1-580 [» ]
2Y1I X-ray 2.78 A 1-580 [» ]
2Y1J X-ray 2.15 A 1-580 [» ]
4B9L X-ray 2.05 A 1-580 [» ]
4B9M X-ray 2.05 A 1-580 [» ]
4B9N X-ray 2.20 A 1-580 [» ]
4B9S X-ray 1.73 A 1-580 [» ]
4B9T X-ray 2.65 A 1-580 [» ]
4B9U X-ray 2.10 A 1-580 [» ]
4B9V X-ray 2.00 A 1-580 [» ]
4DSI X-ray 2.05 A 2-580 [» ]
4DSJ X-ray 2.86 A/B 2-580 [» ]
4DSK X-ray 2.18 A 2-580 [» ]
4DSL X-ray 2.45 A 2-580 [» ]
4DWI X-ray 1.85 A 2-580 [» ]
ProteinModelPortali E1C9K5.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei E1C9K5.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
InterProi IPR002562. 3'-5'_exonuclease_dom.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00476. DNA_pol_A. 1 hit.
[Graphical view ]
PRINTSi PR00868. DNAPOLI.
SMARTi SM00474. 35EXOc. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00593. pola. 1 hit.
PROSITEi PS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Chemical discrimination between dC and 5MedC via their hydroxylamine adducts."
    Munzel M., Lercher L., Muller M., Carell T.
    Nucleic Acids Res. 38:e192-e192(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS).
  2. "Crystal structures and repair studies reveal the identity and the base-pairing properties of the UV-induced spore photoproduct DNA lesion."
    Heil K., Kneuttinger A.C., Schneider S., Lischke U., Carell T.
    Chemistry 17:9651-9657(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
  3. "Reversible bond formation enables the replication and amplification of a crosslinking salen complex as an orthogonal base pair."
    Kaul C., Muller M., Wagner M., Schneider S., Carell T.
    Nat. Chem. 3:794-800(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.22 ANGSTROMS).
  4. "Biochemical and structural insights into the fidelity of bacillus stearothermophilus DNA polymerase."
    Gan J.H., Abdur R., Liu H.H., Sheng J., Caton-Willians J., Soares A.S., Huang Z.
    Submitted (FEB-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.86 ANGSTROMS) OF 2-580.
  5. "Unexpected non-Hoogsteen-based mutagenicity mechanism of FaPy-DNA lesions."
    Gehrke T.H., Lischke U., Gasteiger K.L., Schneider S., Arnold S., Muller H.C., Stephenson D.S., Zipse H., Carell T.
    Nat. Chem. Biol. 9:455-461(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.73 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

Entry informationi

Entry nameiE1C9K5_GEOSE
AccessioniPrimary (citable) accession number: E1C9K5
Entry historyi
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: November 26, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3