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Protein

Ubiquitin carboxyl-terminal hydrolase 47

Gene

USP47

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei196 – 1961NucleophilePROSITE-ProRule annotation
Active sitei502 – 5021Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. base-excision repair Source: UniProtKB
  2. cellular response to DNA damage stimulus Source: UniProtKB
  3. monoubiquitinated protein deubiquitination Source: UniProtKB
  4. negative regulation of transcription, DNA-templated Source: GO_Central
  5. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  6. protein deubiquitination Source: GO_Central
  7. regulation of apoptotic process Source: GO_Central
  8. regulation of proteasomal protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 47 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 47
Ubiquitin thioesterase 47
Ubiquitin-specific-processing protease 47
Gene namesi
Name:USP47
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13751375Ubiquitin carboxyl-terminal hydrolase 47PRO_0000408357Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini187 – 563377USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP47 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

InParanoidiE1C1R4.
OrthoDBiEOG7M3HZD.
PhylomeDBiE1C1R4.
TreeFamiTF314142.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF14560. Ubiquitin_2. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E1C1R4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEFVSMRLL PEDMFWSCRQ STLAEMKKKF AQVESAAEEP RVLCIIQDTT
60 70 80 90 100
NSKTVNERVT LNVPASTPLK KLFEDVASKV GYVNGTFDLV WGNGDNVTDM
110 120 130 140 150
TPIDQNSDKT ILDAGFEPGK KNFLHLTDKD GEQPHIMQEE SGTTEDSAQD
160 170 180 190 200
RFIGPLPREG SVGCTNDYVS QSYSYSSVLS KSETGYVGLV NQAMTCYLNS
210 220 230 240 250
LLQTLFMTPE FRNALYKWEF EESEEDPVTS IPYQLQRLFV LLQTSKKRAI
260 270 280 290 300
ETTDVTRSFG WDSSEAWQQH DVQELCRVMF DALEQKWKQT EQADLINQLY
310 320 330 340 350
QGKLKDYVRC LECGYEGWRI DTYLDIPLVI RPYGSNQAFA SVEEALHAFI
360 370 380 390 400
QPEILDGPNQ YFCERCKKKC DARKGLRFLH FPYLLTLQLK RFDFDYTTMH
410 420 430 440 450
RIKLNDRMTF PEELDMSIFI DVEDEKSPQT ESCTDSGAEN EGSCHSDQMS
460 470 480 490 500
NDFSNDDGVD EGICLESNSA AERIAKVGSE KNSLLYELFS VMVHSGSAAG
510 520 530 540 550
GHYYACIKSF SDDQWYSFND QHVSKITQED IKKTYGGSSG SRGYYSSAFA
560 570 580 590 600
SSTNAYMLIY RLKDPARNAK FLESHEYPDH IKQLVQKERE LEEQEKRQRE
610 620 630 640 650
IERNTCKIKL FCMHPTKQIM MENKLEVHKD RTLKEAVGIA YKLMDLEEAV
660 670 680 690 700
PLDCCRLVKY DEFHDYLERS YEGEEDTPMG LLLGGVKSTY MFDLLLETRR
710 720 730 740 750
PDQIFQCYKP GEVMVKVHVV DLKTESVAPP ISVRAYLNQT VSEFKQLISK
760 770 780 790 800
ATHLPAETMR VVLERCYNDL RLLTVSSKTL KAEGFFRSNK VFIESSESLD
810 820 830 840 850
RHVAYTDSHL WKLLDRHANT IRLYVSLPEQ SPGSQFRRSI YQKPSGDLGN
860 870 880 890 900
LDEACERVKG PAGNMKSVEA ILEESTEKLK SLSLQQQQQE GDNGDSSKST
910 920 930 940 950
EASDFENIES PSNEIDSSAS VENRELENQI QISDPENLQS EERSDSDVNN
960 970 980 990 1000
DRSTSSVDSD ILSSSHSSDT LCNVDNAPIP LANGLDSHSI TSSRRSKANQ
1010 1020 1030 1040 1050
GKKETWDTAE EDSGTDSEYD ESGKSRGETQ YMYFKSEPYT ADEGSGEGQK
1060 1070 1080 1090 1100
WLMVHVDKRI TLSAFKQQLE PFVGVPSSHF KVFRVYASNQ EFESVRLNET
1110 1120 1130 1140 1150
LSSFSDDNKI TIRLGRALKK GEYRVKVYQL LVNEPEPCKF LLDAVFAKGM
1160 1170 1180 1190 1200
TVRQSKEELL PQLREQCGLD LTIDRFRLRK KTWKNPGTVF LDYHIYEEDI
1210 1220 1230 1240 1250
NISSNWEVFL EILDGVEKMK SMSQLAVLSR RWRPSEMKLD SFQEVVLESS
1260 1270 1280 1290 1300
SVEELKEKLS ELSGIPLENI EFAKGRGTFP CDISVLEIHQ DLDWNPKVST
1310 1320 1330 1340 1350
LNVWPLYICD DGAVIFYRDK TEELMELTDE QRNELMKKES SRLQKTGHRV
1360 1370
TYSPRKEKAL KIYLDGAPNK DLTQD
Length:1,375
Mass (Da):157,247
Last modified:November 2, 2010 - v1
Checksum:iE5929D1809146267
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AADN02030606 Genomic DNA. No translation available.
AADN02030607 Genomic DNA. No translation available.
UniGeneiGga.5374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AADN02030606 Genomic DNA. No translation available.
AADN02030607 Genomic DNA. No translation available.
UniGeneiGga.5374.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiE1C1R4.
OrthoDBiEOG7M3HZD.
PhylomeDBiE1C1R4.
TreeFamiTF314142.

Miscellaneous databases

PROiE1C1R4.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF14560. Ubiquitin_2. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution."
    Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A.
    , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K.
    Nature 432:695-716(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiUBP47_CHICK
AccessioniPrimary (citable) accession number: E1C1R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: November 2, 2010
Last modified: March 4, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.