E1C1R4 (UBP47_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 47
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 47
Ubiquitin thioesterase 47
Ubiquitin-specific-processing protease 47

Gene names

Name:

USP47

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP47 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from sequence or structural similarity. Source: UniProtKB cellular response to UV Inferred from electronic annotation. Source: Compara monoubiquitinated protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara positive regulation of cell growth Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	SCF ubiquitin ligase complex Inferred from electronic annotation. Source: Compara cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1375	1375	Ubiquitin carboxyl-terminal hydrolase 47		PRO_0000408357
Sites
	Active site	196	1	Nucleophile By similarity
	Active site	502	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	E1C1R4 [UniParc]. Last modified November 2, 2010. Version 1. Checksum: E5929D1809146267	FASTA	1,375	157,247
	10 20 30 40 50 60 MKEFVSMRLL PEDMFWSCRQ STLAEMKKKF AQVESAAEEP RVLCIIQDTT NSKTVNERVT 70 80 90 100 110 120 LNVPASTPLK KLFEDVASKV GYVNGTFDLV WGNGDNVTDM TPIDQNSDKT ILDAGFEPGK 130 140 150 160 170 180 KNFLHLTDKD GEQPHIMQEE SGTTEDSAQD RFIGPLPREG SVGCTNDYVS QSYSYSSVLS 190 200 210 220 230 240 KSETGYVGLV NQAMTCYLNS LLQTLFMTPE FRNALYKWEF EESEEDPVTS IPYQLQRLFV 250 260 270 280 290 300 LLQTSKKRAI ETTDVTRSFG WDSSEAWQQH DVQELCRVMF DALEQKWKQT EQADLINQLY 310 320 330 340 350 360 QGKLKDYVRC LECGYEGWRI DTYLDIPLVI RPYGSNQAFA SVEEALHAFI QPEILDGPNQ 370 380 390 400 410 420 YFCERCKKKC DARKGLRFLH FPYLLTLQLK RFDFDYTTMH RIKLNDRMTF PEELDMSIFI 430 440 450 460 470 480 DVEDEKSPQT ESCTDSGAEN EGSCHSDQMS NDFSNDDGVD EGICLESNSA AERIAKVGSE 490 500 510 520 530 540 KNSLLYELFS VMVHSGSAAG GHYYACIKSF SDDQWYSFND QHVSKITQED IKKTYGGSSG 550 560 570 580 590 600 SRGYYSSAFA SSTNAYMLIY RLKDPARNAK FLESHEYPDH IKQLVQKERE LEEQEKRQRE 610 620 630 640 650 660 IERNTCKIKL FCMHPTKQIM MENKLEVHKD RTLKEAVGIA YKLMDLEEAV PLDCCRLVKY 670 680 690 700 710 720 DEFHDYLERS YEGEEDTPMG LLLGGVKSTY MFDLLLETRR PDQIFQCYKP GEVMVKVHVV 730 740 750 760 770 780 DLKTESVAPP ISVRAYLNQT VSEFKQLISK ATHLPAETMR VVLERCYNDL RLLTVSSKTL 790 800 810 820 830 840 KAEGFFRSNK VFIESSESLD RHVAYTDSHL WKLLDRHANT IRLYVSLPEQ SPGSQFRRSI 850 860 870 880 890 900 YQKPSGDLGN LDEACERVKG PAGNMKSVEA ILEESTEKLK SLSLQQQQQE GDNGDSSKST 910 920 930 940 950 960 EASDFENIES PSNEIDSSAS VENRELENQI QISDPENLQS EERSDSDVNN DRSTSSVDSD 970 980 990 1000 1010 1020 ILSSSHSSDT LCNVDNAPIP LANGLDSHSI TSSRRSKANQ GKKETWDTAE EDSGTDSEYD 1030 1040 1050 1060 1070 1080 ESGKSRGETQ YMYFKSEPYT ADEGSGEGQK WLMVHVDKRI TLSAFKQQLE PFVGVPSSHF 1090 1100 1110 1120 1130 1140 KVFRVYASNQ EFESVRLNET LSSFSDDNKI TIRLGRALKK GEYRVKVYQL LVNEPEPCKF 1150 1160 1170 1180 1190 1200 LLDAVFAKGM TVRQSKEELL PQLREQCGLD LTIDRFRLRK KTWKNPGTVF LDYHIYEEDI 1210 1220 1230 1240 1250 1260 NISSNWEVFL EILDGVEKMK SMSQLAVLSR RWRPSEMKLD SFQEVVLESS SVEELKEKLS 1270 1280 1290 1300 1310 1320 ELSGIPLENI EFAKGRGTFP CDISVLEIHQ DLDWNPKVST LNVWPLYICD DGAVIFYRDK 1330 1340 1350 1360 1370 TEELMELTDE QRNELMKKES SRLQKTGHRV TYSPRKEKAL KIYLDGAPNK DLTQD

References

[1]

"Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution." Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A. , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K. Nature 432:695-716(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AADN02030606 Genomic DNA. No translation available. AADN02030607 Genomic DNA. No translation available.
IPI	IPI00587332.
UniGene	Gga.5374.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000008932; ENSGALP00000008918; ENSGALG00000005569.
Phylogenomic databases
GeneTree	ENSGT00630000089581.
OMA	NKSETGY.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP47_CHICK

Accession

Primary (citable) accession number: E1C1R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 3, 2011
Last sequence update:	November 2, 2010
Last modified:	March 6, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program