E1BLT8 (ZDHC5_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase ZDHHC5
EC=2.3.1.-

Alternative name(s):
Zinc finger DHHC domain-containing protein 5
Short name=DHHC-5

Gene names

Name:

ZDHHC5

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Palmitoyl acyltransferase for the G-protein coupled receptor SSTR5 and FLOT2 By similarity.
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. Contains 1 DHHC-type zinc finger.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein palmitoylation Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	Palmitoyltransferase ZDHHC5		PRO_0000418363
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Helical; Potential
	Topological domain	35 – 52	18	Extracellular Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Topological domain	74 – 148	75	Cytoplasmic Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Topological domain	170 – 191	22	Extracellular Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Topological domain	213 – 714	502	Cytoplasmic Potential
	Zinc finger	104 – 154	51	DHHC-type
Sites
	Active site	134	1	S-palmitoyl cysteine intermediate By similarity
Amino acid modifications
	Modified residue	91	1	Phosphotyrosine By similarity
	Modified residue	247	1	Phosphoserine By similarity
	Modified residue	296	1	Phosphoserine By similarity
	Modified residue	299	1	Phosphoserine By similarity
	Modified residue	345	1	Phosphoserine By similarity
	Modified residue	348	1	Phosphothreonine By similarity
	Modified residue	380	1	Phosphoserine By similarity
	Modified residue	409	1	Phosphoserine By similarity
	Modified residue	432	1	Phosphoserine By similarity
	Modified residue	436	1	Phosphothreonine By similarity
	Modified residue	529	1	Phosphoserine By similarity
	Modified residue	554	1	Phosphoserine By similarity
	Modified residue	620	1	Phosphoserine By similarity
	Modified residue	658	1	Phosphothreonine By similarity
	Modified residue	693	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	E1BLT8 [UniParc]. Last modified November 2, 2010. Version 1. Checksum: F86040EEF7BFE613	FASTA	714	76,976
	10 20 30 40 50 60 MPAESAKRFK PSKYVPVSAA AIFLVGATTL FFAFTCPGLS LSVSPAVPVY NAVVFLFVLA 70 80 90 100 110 120 NFSMATFMDP GVFPRAEEDE DKEDDFRAPL YKTVEIKGIQ VRMKWCATCR FYRPPRCSHC 130 140 150 160 170 180 SVCDNCVEEF DHHCPWVNNC IGRRNYRYFF LFLLSLTAHI TGVFGFGLLY VLYHMEELSG 190 200 210 220 230 240 VRTAVTMAVM CVAGLFFIPV AGLTGFHVVL VARGRTTNEQ VTGKFRGGVN PFTNGCCNNV 250 260 270 280 290 300 SRVLCSSPAP RYLGRPKKEK TIVIRPPFLR PEVSDGQITV KIMDNGIQGE LRRTKSKGSL 310 320 330 340 350 360 EVTESQSADA EPPPPPKPDL SRYTGLRTHL SLATNEESSL LGKDSPPTPT MYKYRPGYSS 370 380 390 400 410 420 SSASAAMPHS SSAKLSRGDS LKEPPSIAES SRQPSYRSEP SLEPESFRSP TLGKGFPFDP 430 440 450 460 470 480 LSSGSRSSSL KSAQGTGFEL GPLQSIRSEG TTSTSYKSLA NQTRNGSLSY DSLLTPSDSP 490 500 510 520 530 540 DFESVQAGPE PEPPLGYTSP FLSARLAQQR EAERHPRLAP GGPAPREPSP VRYDNLSRHI 550 560 570 580 590 600 VASLQEREKL LRQSPPPGRE EEPGLGDSGV QCTPGSGHAP RTSSSSDDSK RSPLGKTPLA 610 620 630 640 650 660 RPVAPRFGKP DGLRGRGLAS PEPGLPAPYL GRSVSYSSQK APPGVPETEE VALQPLLTPK 670 680 690 700 710 DEVQLKTAYS KSNGQPKSIG SAPPGPGQPP LSSPTRGGVK KVSGVGGTTY EISV

References

[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hereford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DAAA02041769 Genomic DNA. No translation available.
IPI	IPI00688812.
RefSeq	NP_001179692.1. NM_001192763.1.
UniGene	Bt.30828.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000003078; ENSBTAP00000003078; ENSBTAG00000002381.
GeneID	533250.
KEGG	bta:533250.
Organism-specific databases
CTD	25921.
Phylogenomic databases
GeneTree	ENSGT00550000074293.
OMA	VPRFGKP.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20875975.

Entry information

Entry name

ZDHC5_BOVIN

Accession

Primary (citable) accession number: E1BLT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2012
Last sequence update:	November 2, 2010
Last modified:	April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program