E1BKA3 (AJUBA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LIM domain-containing protein ajuba

Gene names

Name:	AJUBA
Synonyms:	JUB

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1 By similarity.
Subunit structure	Interacts with GRB2 and PIP5K1B. Interacts with AURKA; the interaction occurs during mitosis and both proteins are phosphorylated as they form a complex. Interacts with CTNNA1 and with F-actin. Interacts with LATS2; the interaction occurs during mitosis and the complex regulates organization of the spindle apparatus through recruitment of TUBG to the centrosome. Forms a complex with SQSTM1, PRKCZ and TRAF6. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly (via the LIM domains) with GFI1; the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes, and is independent of the GFI1 SNAG domain. Interacts with HDAC1, HDAC2 and HDAC3 By similarity. Interacts with SLC1A2. Interacts with EIF4E, EIF2C1, EIF2C2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with VHL By similarity. Interacts (via LIM domains) with SNAI1 (via SNAG domain), SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain) By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cell membrane By similarity. Cell junction By similarity. Nucleus By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › P-body By similarity. Note: Shuttles between the cytoplasm and the nucleus By similarity. Localizes on centrosomes during G2-M phase By similarity. Colocalizes with GFI1 in the nucleus By similarity. Preferentially co-localizes with cadherin-adhesive complexes at sites of cell-cell contacts By similarity.
Domain	LIM region interacts with CTNNA1. The preLIM region binds directly actin filaments By similarity. LIM-2 and LIM-3 domains mediate the interaction with the N-terminal region of AURKA. The association between LATS2 and AJUBA required the second LIM domain of AJUBA By similarity.
Post-translational modification	Phosphorylated by LATS2 during mitosis. Phosphorylated by AURKA By similarity.
Miscellaneous	'Ajuba' means 'curiosity' in Urdu, an Indian dialect.
Sequence similarities	Contains 3 LIM zinc-binding domains.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing Transcription Transcription regulation
Cellular component	Cell junction Cell membrane Cytoplasm Cytoskeleton Membrane Nucleus
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium-dependent cell-cell adhesion Inferred from electronic annotation. Source: Compara cellular protein localization Inferred from electronic annotation. Source: Compara focal adhesion assembly Inferred from electronic annotation. Source: Compara gene silencing by miRNA Inferred from electronic annotation. Source: Compara glycerophospholipid biosynthetic process Inferred from electronic annotation. Source: Compara lamellipodium assembly Inferred from electronic annotation. Source: Compara negative regulation of hippo signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of kinase activity Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara positive regulation of MAP kinase activity Inferred from electronic annotation. Source: Compara positive regulation of cellular biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of gene silencing by miRNA Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein complex assembly Inferred from electronic annotation. Source: Compara regulation of GTPase activity Inferred from electronic annotation. Source: Compara regulation of cell migration Inferred from electronic annotation. Source: Compara regulation of cellular response to hypoxia Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW wound healing, spreading of epidermal cells Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasmic mRNA processing body Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from electronic annotation. Source: Compara focal adhesion Inferred from electronic annotation. Source: Compara lamellipodium Inferred from electronic annotation. Source: Compara microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell transcription factor complex Inferred from electronic annotation. Source: Compara
Molecular_function	chromatin binding Inferred from electronic annotation. Source: Compara transcription corepressor activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	LIM domain-containing protein ajuba		PRO_0000416966
Regions
	Domain	346 – 407	62	LIM zinc-binding 1
	Domain	411 – 470	60	LIM zinc-binding 2
	Domain	471 – 540	70	LIM zinc-binding 3
Amino acid modifications
	Modified residue	89	1	Phosphoserine By similarity
	Modified residue	129	1	Phosphoserine By similarity
	Modified residue	185	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	E1BKA3 [UniParc]. Last modified November 2, 2010. Version 1. Checksum: 7B29799B3B02CA21	FASTA	548	57,765
	10 20 30 40 50 60 MERLGEKASR LLEKLRLSDS GSAKFGRRKG ESSRSGSDGT PGPGKGRLSG LGGPRKSGPR 70 80 90 100 110 120 GAAGGPGDEP LEPAREQGPL DAERNPRGSF EVPRYEGSFP GGPPPSRALP LPQSLPPDFR 130 140 150 160 170 180 LETTAPALSP RSSFASSSAS DASKPSSPRG SLLLDGAGAG GAGGSRPCSN RTSGISMGYD 190 200 210 220 230 240 QRHGSPLPAG PCLFGPPLAG VPAGYSSGGV PSAYPELHAA LDRLCAHRPA GFGCQESRHS 250 260 270 280 290 300 YPPALGSPGA LAGAGVGATG PLERRGAQPG RHSVTGYGDC AAGARYQDEL TALLRLTVGT 310 320 330 340 350 360 GGREAGVRGE SAGIEASGLE EPPGAFVPEA ARARIREPES REDYFGTCIK CNKGIYGQSN 370 380 390 400 410 420 ACQALDSLYH TQCFVCCSCG RTLRCKAFYS VNGSVYCEED YLFSGFQEAA EKCCVCGHLI 430 440 450 460 470 480 LEKILQAMGK SYHPGCFRCI VCNKCLDGIP FTVDFSNQVY CVTDYHKSYA PKCAACGQPI 490 500 510 520 530 540 LPSEGCEDIV RVISMDRDYH FECYHCEDCR MQLSDEEGCC CFPLDGHLLC HGCHMQRLSA RQPPANYI

References

[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hereford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DAAA02028044 Genomic DNA. No translation available. DAAA02028045 Genomic DNA. No translation available.
IPI	IPI00712757.
RefSeq	NP_001179446.1. NM_001192517.1.
UniGene	Bt.24148.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000016915; ENSBTAP00000016915; ENSBTAG00000012724.
GeneID	519937.
KEGG	bta:519937.
Organism-specific databases
CTD	84962.
Phylogenomic databases
GeneTree	ENSGT00610000085810.
KO	K16682.
OMA	YEGSFPG.
Family and domain databases
Gene3D	2.10.110.10. 3 hits.
InterPro	IPR001781. Znf_LIM. [Graphical view]
Pfam	PF00412. LIM. 3 hits. [Graphical view]
SMART	SM00132. LIM. 3 hits. [Graphical view]
PROSITE	PS50023. LIM_DOMAIN_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20872985.

Entry information

Entry name

AJUBA_BOVIN

Accession

Primary (citable) accession number: E1BKA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2012
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program