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Protein

Cyclin-dependent kinase 12

Gene

CDK12

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors (By similarity).By similarity

Catalytic activityi

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei756ATPPROSITE-ProRule annotation1
Active sitei859Proton acceptorPROSITE-ProRule annotation1
Binding sitei1042ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi733 – 741ATPPROSITE-ProRule annotation9
Nucleotide bindingi814 – 819ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 12 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 12
Gene namesi
Name:CDK12
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity
  • Nucleus speckle By similarity

  • Note: Colocalized with nuclear speckles throughout interphase.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004069581 – 1264Cyclin-dependent kinase 12Add BLAST1264

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphothreonineBy similarity1
Modified residuei73PhosphotyrosineBy similarity1
Modified residuei236PhosphoserineBy similarity1
Modified residuei249PhosphoserineBy similarity1
Modified residuei265PhosphoserineBy similarity1
Modified residuei274PhosphoserineBy similarity1
Modified residuei276PhosphoserineBy similarity1
Modified residuei301PhosphoserineBy similarity1
Modified residuei303PhosphoserineBy similarity1
Modified residuei310PhosphoserineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei318PhosphoserineBy similarity1
Modified residuei323PhosphoserineBy similarity1
Modified residuei325PhosphoserineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Modified residuei334PhosphoserineBy similarity1
Modified residuei338PhosphoserineBy similarity1
Modified residuei341PhosphoserineBy similarity1
Modified residuei343PhosphoserineBy similarity1
Modified residuei345PhosphoserineBy similarity1
Modified residuei383PhosphoserineBy similarity1
Modified residuei385PhosphoserineBy similarity1
Modified residuei400PhosphoserineBy similarity1
Modified residuei420PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei515PhosphothreonineBy similarity1
Modified residuei614PhosphoserineBy similarity1
Modified residuei644PhosphoserineBy similarity1
Modified residuei681PhosphoserineBy similarity1
Modified residuei685PhosphoserineBy similarity1
Modified residuei692PhosphothreonineBy similarity1
Modified residuei889PhosphoserineBy similarity1
Modified residuei893PhosphothreonineBy similarity1
Modified residuei1055PhosphoserineBy similarity1
Modified residuei1085PhosphoserineBy similarity1
Modified residuei1246PhosphothreonineBy similarity1
Modified residuei1248PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Thr-893 increases kinase activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiE1BB50.
PRIDEiE1BB50.

Interactioni

Subunit structurei

Interacts with CCNL1 and CCNL2.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002005.

Structurei

3D structure databases

ProteinModelPortaliE1BB50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini727 – 1022Protein kinasePROSITE-ProRule annotationAdd BLAST296

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi137 – 394Ser-richAdd BLAST258
Compositional biasi407 – 413Poly-Ala7
Compositional biasi528 – 707Pro-richAdd BLAST180
Compositional biasi1062 – 1065Poly-Pro4

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0600. Eukaryota.
ENOG410XPIR. LUCA.
InParanoidiE1BB50.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E1BB50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNPERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLGS KHKRHKSKHS
60 70 80 90 100
KDMGLVTPEA APLGTVIKPL VEYDDISSDS DTFSDDLAFK VDRRENDERR
110 120 130 140 150
GTDRSDRLHK HRHHQHRRTR DLLKTKQTEK EKNLEASSKS GSTKDRISGS
160 170 180 190 200
SKRSNEENDD HGKAQISKSS NKESRSSKLH KEKTRKEREL KSGHKDRSKS
210 220 230 240 250
HRKRETPKSY KTVDSPKRRS RSPHRKWSDS PKQDDSPSGA SYGQDYDLSP
260 270 280 290 300
PRSHTSSNYD SYKKSPGSTS RRQSISPPYK EPSAYQSSTR SPSPYSRRQR
310 320 330 340 350
SVSPYSRRRS SSYERSGSYS GRSPSPYGRR RSSSPFLSKR SLSRSPLPSR
360 370 380 390 400
KSMKSRSRSP AYSRHSSSHS KKKRSGSRSR HSSISPVRLP LNSSLGAELS
410 420 430 440 450
RKKKERAAAA AAAKMDGKES KGSPIFLPKK ENSSVEAKDS GLEPKKLPRG
460 470 480 490 500
VKLEKSAPDT ELVNVTHLNT EVKNSLDTGK VKLDENSEKH PVLKDTKVQG
510 520 530 540 550
TKDSKPVALK EEIVTPKETE TSEKETLPPL PTVTSPPPLP TTTPPPQTPP
560 570 580 590 600
LPPLPPLPAI PQQPPLPPPQ PTFSQVLSSS TSTLPPSVHP RTSTLSSQAN
610 620 630 640 650
SQPSVQVSVK TQVSVTAAIP HLKTSTLPPL PLPPLLLGDD DMDSPKETLP
660 670 680 690 700
SKPVKKEKEQ RPRHLLTDLP LPPELPGGDP SPPDSPEPKA ITPPQQPYKK
710 720 730 740 750
RPKICCPRYG ERRQTESDWG KRCVDKFDII GIIGEGTYGQ VYKAKDKDTG
760 770 780 790 800
ELVALKKVRL DNEKEGFPIT AIREIKILRQ LIHRSVVNMK EIVTDKQDAL
810 820 830 840 850
DFKKDKGAFY LVFEYMDHDL MGLLESGLVH FSEDHIKSFM KQLMEGLDYC
860 870 880 890 900
HKKNFLHRDI KCSNILLNNS GQIKLADFGL ARLYNSEESR PYTNKVITLW
910 920 930 940 950
YRPPELLLGE ERYTPAIDVW SCGCILGELF TKKPIFQANL ELAQLELISR
960 970 980 990 1000
LCGSPCPAVW PDVIKLPYFN TMKPKKQYRR RLREEFSFFF LPWGALDLLD
1010 1020 1030 1040 1050
HMLTLDPSKR CTAEQTLQSD FLKDVELSKM DPPDLPHWQD CHELWSKKRR
1060 1070 1080 1090 1100
RQRQSGVLVE EPPPPKASRK ETISGTSAEP VKNSSPAPPQ PAPGKVEPGA
1110 1120 1130 1140 1150
GDAIGLGDIT QQLNQSELAV LLNLLQSQTD LSIPQMAQLL NIHSNPEMQQ
1160 1170 1180 1190 1200
QLEALNQSIS ALTAATSQQQ DSEPTAPEES LKEIAPAPAV QPSAEQTTPE
1210 1220 1230 1240 1250
ASSTPADMQN MLAVLLSQLM KTQEPAGNLE ENNSDKNSGP QGPRRTPTMP
1260
QEEAAGKQTG HESH
Length:1,264
Mass (Da):140,642
Last modified:November 2, 2010 - v1
Checksum:iA074D89C354DBAC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFC03033685 Genomic DNA. No translation available.
AAFC03036812 Genomic DNA. No translation available.
AAFC03053477 Genomic DNA. No translation available.
AAFC03053478 Genomic DNA. No translation available.
UniGeneiBt.67418.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFC03033685 Genomic DNA. No translation available.
AAFC03036812 Genomic DNA. No translation available.
AAFC03053477 Genomic DNA. No translation available.
AAFC03053478 Genomic DNA. No translation available.
UniGeneiBt.67418.

3D structure databases

ProteinModelPortaliE1BB50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002005.

Proteomic databases

PaxDbiE1BB50.
PRIDEiE1BB50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0600. Eukaryota.
ENOG410XPIR. LUCA.
InParanoidiE1BB50.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDK12_BOVIN
AccessioniPrimary (citable) accession number: E1BB50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: November 2, 2010
Last modified: July 6, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.