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E1BB50

- CDK12_BOVIN

UniProt

E1BB50 - CDK12_BOVIN

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Protein
Cyclin-dependent kinase 12
Gene
CDK12
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors By similarity.

Catalytic activityi

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei756 – 7561ATP By similarity
Active sitei859 – 8591Proton acceptor By similarity
Binding sitei1042 – 10421ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi733 – 7419ATP By similarity
Nucleotide bindingi814 – 8196ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB
  3. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. RNA splicing Source: UniProtKB
  2. mRNA processing Source: UniProtKB-KW
  3. phosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
  4. regulation of MAP kinase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 12 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Cell division protein kinase 12
Gene namesi
Name:CDK12
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Nucleus By similarity. Nucleus speckle By similarity
Note: Colocalized with nuclear speckles throughout interphase By similarity.

GO - Cellular componenti

  1. nuclear cyclin-dependent protein kinase holoenzyme complex Source: UniProtKB
  2. nuclear speck Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12641264Cyclin-dependent kinase 12
PRO_0000406958Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571Phosphothreonine By similarity
Modified residuei73 – 731Phosphotyrosine By similarity
Modified residuei236 – 2361Phosphoserine By similarity
Modified residuei249 – 2491Phosphoserine By similarity
Modified residuei274 – 2741Phosphoserine By similarity
Modified residuei276 – 2761Phosphoserine By similarity
Modified residuei301 – 3011Phosphoserine By similarity
Modified residuei303 – 3031Phosphoserine By similarity
Modified residuei310 – 3101Phosphoserine By similarity
Modified residuei312 – 3121Phosphoserine By similarity
Modified residuei318 – 3181Phosphoserine By similarity
Modified residuei323 – 3231Phosphoserine By similarity
Modified residuei325 – 3251Phosphoserine By similarity
Modified residuei332 – 3321Phosphoserine By similarity
Modified residuei333 – 3331Phosphoserine By similarity
Modified residuei334 – 3341Phosphoserine By similarity
Modified residuei338 – 3381Phosphoserine By similarity
Modified residuei345 – 3451Phosphoserine By similarity
Modified residuei383 – 3831Phosphoserine By similarity
Modified residuei385 – 3851Phosphoserine By similarity
Modified residuei400 – 4001Phosphoserine By similarity
Modified residuei420 – 4201Phosphoserine By similarity
Modified residuei423 – 4231Phosphoserine By similarity
Modified residuei515 – 5151Phosphothreonine By similarity
Modified residuei681 – 6811Phosphoserine By similarity
Modified residuei685 – 6851Phosphoserine By similarity
Modified residuei692 – 6921Phosphothreonine By similarity
Modified residuei893 – 8931Phosphothreonine By similarity
Modified residuei1055 – 10551Phosphoserine By similarity
Modified residuei1085 – 10851Phosphoserine By similarity
Modified residuei1246 – 12461Phosphothreonine By similarity

Post-translational modificationi

Phosphorylation at Thr-893 increases kinase activity By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiE1BB50.

Interactioni

Subunit structurei

Interacts with CCNL1 and CCNL2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliE1BB50.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini727 – 1022296Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi137 – 394258Ser-rich
Add
BLAST
Compositional biasi407 – 4137Poly-Ala
Compositional biasi528 – 707180Pro-rich
Add
BLAST
Compositional biasi1062 – 10654Poly-Pro

Sequence similaritiesi

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E1BB50-1 [UniParc]FASTAAdd to Basket

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MPNPERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLGS KHKRHKSKHS     50
KDMGLVTPEA APLGTVIKPL VEYDDISSDS DTFSDDLAFK VDRRENDERR 100
GTDRSDRLHK HRHHQHRRTR DLLKTKQTEK EKNLEASSKS GSTKDRISGS 150
SKRSNEENDD HGKAQISKSS NKESRSSKLH KEKTRKEREL KSGHKDRSKS 200
HRKRETPKSY KTVDSPKRRS RSPHRKWSDS PKQDDSPSGA SYGQDYDLSP 250
PRSHTSSNYD SYKKSPGSTS RRQSISPPYK EPSAYQSSTR SPSPYSRRQR 300
SVSPYSRRRS SSYERSGSYS GRSPSPYGRR RSSSPFLSKR SLSRSPLPSR 350
KSMKSRSRSP AYSRHSSSHS KKKRSGSRSR HSSISPVRLP LNSSLGAELS 400
RKKKERAAAA AAAKMDGKES KGSPIFLPKK ENSSVEAKDS GLEPKKLPRG 450
VKLEKSAPDT ELVNVTHLNT EVKNSLDTGK VKLDENSEKH PVLKDTKVQG 500
TKDSKPVALK EEIVTPKETE TSEKETLPPL PTVTSPPPLP TTTPPPQTPP 550
LPPLPPLPAI PQQPPLPPPQ PTFSQVLSSS TSTLPPSVHP RTSTLSSQAN 600
SQPSVQVSVK TQVSVTAAIP HLKTSTLPPL PLPPLLLGDD DMDSPKETLP 650
SKPVKKEKEQ RPRHLLTDLP LPPELPGGDP SPPDSPEPKA ITPPQQPYKK 700
RPKICCPRYG ERRQTESDWG KRCVDKFDII GIIGEGTYGQ VYKAKDKDTG 750
ELVALKKVRL DNEKEGFPIT AIREIKILRQ LIHRSVVNMK EIVTDKQDAL 800
DFKKDKGAFY LVFEYMDHDL MGLLESGLVH FSEDHIKSFM KQLMEGLDYC 850
HKKNFLHRDI KCSNILLNNS GQIKLADFGL ARLYNSEESR PYTNKVITLW 900
YRPPELLLGE ERYTPAIDVW SCGCILGELF TKKPIFQANL ELAQLELISR 950
LCGSPCPAVW PDVIKLPYFN TMKPKKQYRR RLREEFSFFF LPWGALDLLD 1000
HMLTLDPSKR CTAEQTLQSD FLKDVELSKM DPPDLPHWQD CHELWSKKRR 1050
RQRQSGVLVE EPPPPKASRK ETISGTSAEP VKNSSPAPPQ PAPGKVEPGA 1100
GDAIGLGDIT QQLNQSELAV LLNLLQSQTD LSIPQMAQLL NIHSNPEMQQ 1150
QLEALNQSIS ALTAATSQQQ DSEPTAPEES LKEIAPAPAV QPSAEQTTPE 1200
ASSTPADMQN MLAVLLSQLM KTQEPAGNLE ENNSDKNSGP QGPRRTPTMP 1250
QEEAAGKQTG HESH 1264
Length:1,264
Mass (Da):140,642
Last modified:November 2, 2010 - v1
Checksum:iA074D89C354DBAC7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFC03033685 Genomic DNA. No translation available.
AAFC03036812 Genomic DNA. No translation available.
AAFC03053477 Genomic DNA. No translation available.
AAFC03053478 Genomic DNA. No translation available.
UniGeneiBt.67418.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFC03033685 Genomic DNA. No translation available.
AAFC03036812 Genomic DNA. No translation available.
AAFC03053477 Genomic DNA. No translation available.
AAFC03053478 Genomic DNA. No translation available.
UniGenei Bt.67418.

3D structure databases

ProteinModelPortali E1BB50.
ModBasei Search...

Proteomic databases

PRIDEi E1BB50.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of taurine cattle: a window to ruminant biology and evolution."
    The bovine genome sequencing and analysis consortium
    Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiCDK12_BOVIN
AccessioniPrimary (citable) accession number: E1BB50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: November 2, 2010
Last modified: June 11, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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