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E1BB50 (CDK12_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase 12

EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division protein kinase 12
Gene names
Name:CDK12
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length1264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors By similarity.

Catalytic activity

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with CCNL1 and CCNL2 By similarity.

Subcellular location

Nucleus By similarity. Nucleus speckle By similarity. Note: Colocalized with nuclear speckles throughout interphase By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12641264Cyclin-dependent kinase 12
PRO_0000406958

Regions

Domain727 – 1022296Protein kinase
Nucleotide binding733 – 7419ATP By similarity
Compositional bias137 – 394258Ser-rich
Compositional bias407 – 4137Poly-Ala
Compositional bias528 – 707180Pro-rich
Compositional bias1062 – 10654Poly-Pro

Sites

Active site8591Proton acceptor By similarity
Binding site7561ATP By similarity

Amino acid modifications

Modified residue571Phosphothreonine By similarity
Modified residue731Phosphotyrosine By similarity
Modified residue2361Phosphoserine By similarity
Modified residue2491Phosphoserine By similarity
Modified residue2741Phosphoserine By similarity
Modified residue2761Phosphoserine By similarity
Modified residue3011Phosphoserine By similarity
Modified residue3031Phosphoserine By similarity
Modified residue3101Phosphoserine By similarity
Modified residue3121Phosphoserine By similarity
Modified residue3181Phosphoserine By similarity
Modified residue3231Phosphoserine By similarity
Modified residue3251Phosphoserine By similarity
Modified residue3321Phosphoserine By similarity
Modified residue3331Phosphoserine By similarity
Modified residue3341Phosphoserine By similarity
Modified residue3381Phosphoserine By similarity
Modified residue3451Phosphoserine By similarity
Modified residue3831Phosphoserine By similarity
Modified residue3851Phosphoserine By similarity
Modified residue4001Phosphoserine By similarity
Modified residue4201Phosphoserine By similarity
Modified residue4231Phosphoserine By similarity
Modified residue5151Phosphothreonine By similarity
Modified residue6811Phosphoserine By similarity
Modified residue6851Phosphoserine By similarity
Modified residue6921Phosphothreonine By similarity
Modified residue8931Phosphothreonine By similarity
Modified residue10551Phosphoserine By similarity
Modified residue10851Phosphoserine By similarity
Modified residue12461Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
E1BB50 [UniParc].

Last modified November 2, 2010. Version 1.
Checksum: A074D89C354DBAC7

FASTA1,264140,642
        10         20         30         40         50         60 
MPNPERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLGS KHKRHKSKHS KDMGLVTPEA 

        70         80         90        100        110        120 
APLGTVIKPL VEYDDISSDS DTFSDDLAFK VDRRENDERR GTDRSDRLHK HRHHQHRRTR 

       130        140        150        160        170        180 
DLLKTKQTEK EKNLEASSKS GSTKDRISGS SKRSNEENDD HGKAQISKSS NKESRSSKLH 

       190        200        210        220        230        240 
KEKTRKEREL KSGHKDRSKS HRKRETPKSY KTVDSPKRRS RSPHRKWSDS PKQDDSPSGA 

       250        260        270        280        290        300 
SYGQDYDLSP PRSHTSSNYD SYKKSPGSTS RRQSISPPYK EPSAYQSSTR SPSPYSRRQR 

       310        320        330        340        350        360 
SVSPYSRRRS SSYERSGSYS GRSPSPYGRR RSSSPFLSKR SLSRSPLPSR KSMKSRSRSP 

       370        380        390        400        410        420 
AYSRHSSSHS KKKRSGSRSR HSSISPVRLP LNSSLGAELS RKKKERAAAA AAAKMDGKES 

       430        440        450        460        470        480 
KGSPIFLPKK ENSSVEAKDS GLEPKKLPRG VKLEKSAPDT ELVNVTHLNT EVKNSLDTGK 

       490        500        510        520        530        540 
VKLDENSEKH PVLKDTKVQG TKDSKPVALK EEIVTPKETE TSEKETLPPL PTVTSPPPLP 

       550        560        570        580        590        600 
TTTPPPQTPP LPPLPPLPAI PQQPPLPPPQ PTFSQVLSSS TSTLPPSVHP RTSTLSSQAN 

       610        620        630        640        650        660 
SQPSVQVSVK TQVSVTAAIP HLKTSTLPPL PLPPLLLGDD DMDSPKETLP SKPVKKEKEQ 

       670        680        690        700        710        720 
RPRHLLTDLP LPPELPGGDP SPPDSPEPKA ITPPQQPYKK RPKICCPRYG ERRQTESDWG 

       730        740        750        760        770        780 
KRCVDKFDII GIIGEGTYGQ VYKAKDKDTG ELVALKKVRL DNEKEGFPIT AIREIKILRQ 

       790        800        810        820        830        840 
LIHRSVVNMK EIVTDKQDAL DFKKDKGAFY LVFEYMDHDL MGLLESGLVH FSEDHIKSFM 

       850        860        870        880        890        900 
KQLMEGLDYC HKKNFLHRDI KCSNILLNNS GQIKLADFGL ARLYNSEESR PYTNKVITLW 

       910        920        930        940        950        960 
YRPPELLLGE ERYTPAIDVW SCGCILGELF TKKPIFQANL ELAQLELISR LCGSPCPAVW 

       970        980        990       1000       1010       1020 
PDVIKLPYFN TMKPKKQYRR RLREEFSFFF LPWGALDLLD HMLTLDPSKR CTAEQTLQSD 

      1030       1040       1050       1060       1070       1080 
FLKDVELSKM DPPDLPHWQD CHELWSKKRR RQRQSGVLVE EPPPPKASRK ETISGTSAEP 

      1090       1100       1110       1120       1130       1140 
VKNSSPAPPQ PAPGKVEPGA GDAIGLGDIT QQLNQSELAV LLNLLQSQTD LSIPQMAQLL 

      1150       1160       1170       1180       1190       1200 
NIHSNPEMQQ QLEALNQSIS ALTAATSQQQ DSEPTAPEES LKEIAPAPAV QPSAEQTTPE 

      1210       1220       1230       1240       1250       1260 
ASSTPADMQN MLAVLLSQLM KTQEPAGNLE ENNSDKNSGP QGPRRTPTMP QEEAAGKQTG 


HESH 

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References

[1]"The genome sequence of taurine cattle: a window to ruminant biology and evolution."
The bovine genome sequencing and analysis consortium
Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFC03033685 Genomic DNA. No translation available.
AAFC03036812 Genomic DNA. No translation available.
AAFC03053477 Genomic DNA. No translation available.
AAFC03053478 Genomic DNA. No translation available.
UniGeneBt.67418.

3D structure databases

ProteinModelPortalE1BB50.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEE1BB50.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDK12_BOVIN
AccessionPrimary (citable) accession number: E1BB50
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: November 2, 2010
Last modified: April 16, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families