Cyclin-dependent kinase 12 - Bos taurus (Bovine)

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E1BB50 (CDK12_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 18. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 12
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division protein kinase 12

Gene names

Name:

CDK12

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors By similarity.
Catalytic activity	ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with CCNL1 and CCNL2 By similarity.
Subcellular location	Nucleus By similarity. Nucleus speckle By similarity. Note: Colocalized with nuclear speckles throughout interphase By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from sequence or structural similarity. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: GOC mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation of RNA polymerase II C-terminal domain Inferred from sequence or structural similarity. Source: UniProtKB regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nuclear cyclin-dependent protein kinase holoenzyme complex Inferred from sequence or structural similarity. Source: UniProtKB nuclear speck Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from sequence or structural similarity. Source: UniProtKB cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1264

1264

Cyclin-dependent kinase 12

PRO_0000406958

Regions

Domain

727 – 1022

296

Protein kinase

Nucleotide binding

733 – 741

ATP By similarity

Compositional bias

137 – 394

258

Ser-rich

Compositional bias

407 – 413

Poly-Ala

Compositional bias

528 – 707

180

Pro-rich

Compositional bias

1062 – 1065

Poly-Pro

Sites

Active site

859

Proton acceptor By similarity

Binding site

756

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

236

Phosphoserine By similarity

Modified residue

249

Phosphoserine By similarity

Modified residue

274

Phosphoserine By similarity

Modified residue

276

Phosphoserine By similarity

Modified residue

279

Phosphotyrosine By similarity

Modified residue

301

Phosphoserine By similarity

Modified residue

303

Phosphoserine By similarity

Modified residue

310

Phosphoserine By similarity

Modified residue

312

Phosphoserine By similarity

Modified residue

318

Phosphoserine By similarity

Modified residue

319

Phosphotyrosine By similarity

Modified residue

323

Phosphoserine By similarity

Modified residue

325

Phosphoserine By similarity

Modified residue

332

Phosphoserine By similarity

Modified residue

333

Phosphoserine By similarity

Modified residue

334

Phosphoserine By similarity

Modified residue

338

Phosphoserine By similarity

Modified residue

345

Phosphoserine By similarity

Modified residue

383

Phosphoserine By similarity

Modified residue

385

Phosphoserine By similarity

Modified residue

400

Phosphoserine By similarity

Modified residue

420

Phosphoserine By similarity

Modified residue

423

Phosphoserine By similarity

Modified residue

515

Phosphothreonine By similarity

Modified residue

681

Phosphoserine By similarity

Modified residue

685

Phosphoserine By similarity

Modified residue

692

Phosphothreonine By similarity

Modified residue

893

Phosphothreonine By similarity

Modified residue

1055

Phosphoserine By similarity

Modified residue

1085

Phosphoserine By similarity

Modified residue

1246

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

E1BB50 [UniParc].

Last modified November 2, 2010. Version 1.
Checksum: A074D89C354DBAC7
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FASTA

1,264

140,642

        10         20         30         40         50         60 
MPNPERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLGS KHKRHKSKHS KDMGLVTPEA 

        70         80         90        100        110        120 
APLGTVIKPL VEYDDISSDS DTFSDDLAFK VDRRENDERR GTDRSDRLHK HRHHQHRRTR 

       130        140        150        160        170        180 
DLLKTKQTEK EKNLEASSKS GSTKDRISGS SKRSNEENDD HGKAQISKSS NKESRSSKLH 

       190        200        210        220        230        240 
KEKTRKEREL KSGHKDRSKS HRKRETPKSY KTVDSPKRRS RSPHRKWSDS PKQDDSPSGA 

       250        260        270        280        290        300 
SYGQDYDLSP PRSHTSSNYD SYKKSPGSTS RRQSISPPYK EPSAYQSSTR SPSPYSRRQR 

       310        320        330        340        350        360 
SVSPYSRRRS SSYERSGSYS GRSPSPYGRR RSSSPFLSKR SLSRSPLPSR KSMKSRSRSP 

       370        380        390        400        410        420 
AYSRHSSSHS KKKRSGSRSR HSSISPVRLP LNSSLGAELS RKKKERAAAA AAAKMDGKES 

       430        440        450        460        470        480 
KGSPIFLPKK ENSSVEAKDS GLEPKKLPRG VKLEKSAPDT ELVNVTHLNT EVKNSLDTGK 

       490        500        510        520        530        540 
VKLDENSEKH PVLKDTKVQG TKDSKPVALK EEIVTPKETE TSEKETLPPL PTVTSPPPLP 

       550        560        570        580        590        600 
TTTPPPQTPP LPPLPPLPAI PQQPPLPPPQ PTFSQVLSSS TSTLPPSVHP RTSTLSSQAN 

       610        620        630        640        650        660 
SQPSVQVSVK TQVSVTAAIP HLKTSTLPPL PLPPLLLGDD DMDSPKETLP SKPVKKEKEQ 

       670        680        690        700        710        720 
RPRHLLTDLP LPPELPGGDP SPPDSPEPKA ITPPQQPYKK RPKICCPRYG ERRQTESDWG 

       730        740        750        760        770        780 
KRCVDKFDII GIIGEGTYGQ VYKAKDKDTG ELVALKKVRL DNEKEGFPIT AIREIKILRQ 

       790        800        810        820        830        840 
LIHRSVVNMK EIVTDKQDAL DFKKDKGAFY LVFEYMDHDL MGLLESGLVH FSEDHIKSFM 

       850        860        870        880        890        900 
KQLMEGLDYC HKKNFLHRDI KCSNILLNNS GQIKLADFGL ARLYNSEESR PYTNKVITLW 

       910        920        930        940        950        960 
YRPPELLLGE ERYTPAIDVW SCGCILGELF TKKPIFQANL ELAQLELISR LCGSPCPAVW 

       970        980        990       1000       1010       1020 
PDVIKLPYFN TMKPKKQYRR RLREEFSFFF LPWGALDLLD HMLTLDPSKR CTAEQTLQSD 

      1030       1040       1050       1060       1070       1080 
FLKDVELSKM DPPDLPHWQD CHELWSKKRR RQRQSGVLVE EPPPPKASRK ETISGTSAEP 

      1090       1100       1110       1120       1130       1140 
VKNSSPAPPQ PAPGKVEPGA GDAIGLGDIT QQLNQSELAV LLNLLQSQTD LSIPQMAQLL 

      1150       1160       1170       1180       1190       1200 
NIHSNPEMQQ QLEALNQSIS ALTAATSQQQ DSEPTAPEES LKEIAPAPAV QPSAEQTTPE 

      1210       1220       1230       1240       1250       1260 
ASSTPADMQN MLAVLLSQLM KTQEPAGNLE ENNSDKNSGP QGPRRTPTMP QEEAAGKQTG 


HESH

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References

[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFC03033685 Genomic DNA. No translation available. AAFC03036812 Genomic DNA. No translation available. AAFC03053477 Genomic DNA. No translation available. AAFC03053478 Genomic DNA. No translation available.
IPI	IPI00717875.
UniGene	Bt.67418.
3D structure databases
ProteinModelPortal	E1BB50.
ModBase	Search...
Proteomic databases
PRIDE	E1BB50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDK12_BOVIN

Accession

Primary (citable) accession number: E1BB50

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 5, 2011
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Family and domain databases

Entry information

Relevant documents