E1B9W9 (UBP42_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 42
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 42
Ubiquitin thioesterase 42
Ubiquitin-specific-processing protease 42

Gene names

Name:

USP42

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	1333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Deubiquitinating enzyme which may play an important role during spermatogenesis By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family.

Ontologies

Keywords
Biological process	Differentiation Spermatogenesis Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1333	1333	Ubiquitin carboxyl-terminal hydrolase 42		PRO_0000404198
Regions
	Compositional bias	64 – 67	4	Poly-Ser
	Compositional bias	952 – 1126	175	Arg-rich
	Compositional bias	1185 – 1255	71	Lys-rich
Sites
	Active site	120	1	Nucleophile By similarity
	Active site	371	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	863	1	Phosphoserine By similarity
	Modified residue	1235	1	Phosphoserine By similarity
	Modified residue	1256	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	E1B9W9 [UniParc]. Last modified November 2, 2010. Version 1. Checksum: 8E638010675EA919	FASTA	1,333	146,748
	10 20 30 40 50 60 MTIVDKASES SDPSTYQNQP GSSEAVSPGD MDAGSASWGA VSSLNDVSNH TLSLGPVPGA 70 80 90 100 110 120 VVYSSSSVPE KSKPSPQKDQ ALGDGIAPPQ KVLFPSEKIC LKWQQTHRVG AGLQNLGNTC 130 140 150 160 170 180 FANAALQCLT YTPPLANYML SHEHSKTCHA EGFCMMCTMQ AHITQALSNP GDVIKPMFVI 190 200 210 220 230 240 NEMRRIARHF RFGNQEDAHE FLQYTVDAMQ KACLNGSNKL DRHTQATTLV CQIFGGYLRS 250 260 270 280 290 300 RVKCLNCKGV SDTFDPYLDI TLEIKAAQSV NKALEQFVKP EQLDGENSYK CSKCKKMVPA 310 320 330 340 350 360 SKRFTIHRSS NVLTLSLKRF ANFTGGKIAK DVKYPEYLDI RPYMSQPNGE PIIYVLYAVL 370 380 390 400 410 420 VHTGFNCHAG HYFCYIKASN GLWYQMNDSI VSTSDIRSVL SQQAYVLFYI RSHDVKNGGE 430 440 450 460 470 480 LTHSAHSPGQ SSPRPVISQR VVNSKQAASG FIGPQLPSHM MKNPPHLNGT GPLKETPSSP 490 500 510 520 530 540 MSGPSGNSSV SRTGPVSASP SVQNWSVNRP SVIPEHPKKQ KITISIHNKL PVRQGQSQSN 550 560 570 580 590 600 LHSNSLEHPN KPAPSSTITT SSAIQSTSSA PTPPASSKVP KQMAPREACS RPMMNGRPRL 610 620 630 640 650 660 SAGVLVPYGA ESSEESDEEA KGLGQENGRG LTESACSPAL DAEDGDASPH ELQEPVALNG 670 680 690 700 710 720 ATGLDSDPTE NGLPSDGAPC PGQPALHSEH PFPKANGLPG KLMPAPLPPL PEDKILETFK 730 740 750 760 770 780 LGSKAKGSAE ETRTPGSEER PLEHPEAELE AGRSPPGDAR DNLESVSNLS SKFKKASPPS 790 800 810 820 830 840 DPSIKPTKEE VSERVSAEPE EAVPSASTFC NPDKDALGDD QLLAPCDPGN LTDDQSKPSP 850 860 870 880 890 900 DVGGTLPERP QDPVAAEAAG RLSPGPSVPS EGDHEPEPLG HSGRDRGSDA EGPSKSMGVS 910 920 930 940 950 960 TDEAPSAQLD TITENRPEGP PERSSSERGE DSKAGQKAPE PCLVQEKVSS LRKVDRGHYR 970 980 990 1000 1010 1020 SRRDRSSSGE HARESRSRTE VQHRRKRPHR EHERPRPERP RPEPCALAPQ PPCPSSLARS 1030 1040 1050 1060 1070 1080 GHHHSRSRGA PDQEWGRYHH AEGDHAWTRE KYYPDRLRWE RCRYHHDRSP LYPSREPRDW 1090 1100 1110 1120 1130 1140 RPFHAEREYE RAGPYGGRPY KDHYRGRKGY ELVAKERDRH RFSSPRAGMA HAPPPHPAAK 1150 1160 1170 1180 1190 1200 YTHDRLSFGA EDGSCDLAAR FHEHDNIKSR KRRYDSLENE SHVEKKAWRS LQKDPVEEPK 1210 1220 1230 1240 1250 1260 VKKHKKSKKK KKSKDKHRDR DSRHQQDSDL SVAHSDADLH RHKKKKKKKK RHSRKSEDFG 1270 1280 1290 1300 1310 1320 RDSEPRLPKA ASCETVDHFR KAEGAFPLAD GLPLEGAAPF CEKTKHFRME SREVRCRLSQ 1330 CDQGKGARWE SGS

References

[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFC03050082 Genomic DNA. No translation available.
IPI	IPI00711714.
UniGene	Bt.42050.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000000967; ENSBTAP00000000967; ENSBTAG00000000725.
Phylogenomic databases
GeneTree	ENSGT00670000097591.
OMA	ALHSENP.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP42_BOVIN

Accession

Primary (citable) accession number: E1B9W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	November 2, 2010
Last modified:	March 6, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program