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Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1

Gene

SMARCAD1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi523 – 531ATPPROSITE-ProRule annotation9
Nucleotide bindingi899 – 906ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 (EC:3.6.4.12)
Gene namesi
Name:SMARCAD1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componentsi: Chromosome 6, Unassembled WGS sequence

Subcellular locationi

  • Nucleus By similarity
  • Chromosome By similarity

  • Note: Colocalizes with PCNA at replication forks during S phase. Recruited to double-strand breaks (DSBs) sites of DNA damage (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004169331 – 1028SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1Add BLAST1028

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei54PhosphothreonineBy similarity1
Modified residuei57PhosphoserineBy similarity1
Modified residuei71PhosphothreonineBy similarity1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei79PhosphoserineBy similarity1
Modified residuei124PhosphoserineBy similarity1
Modified residuei127PhosphoserineBy similarity1
Modified residuei132PhosphoserineBy similarity1
Modified residuei153PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei241PhosphoserineBy similarity1
Modified residuei244PhosphoserineBy similarity1
Modified residuei304PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiE1B7X9.
PRIDEiE1B7X9.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017061.

Interactioni

Subunit structurei

Binds to DNA preferentially in the vicinity of transcriptional start sites. Interacts with MSH2 and TRIM28. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with PCNA (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000045084.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini158 – 200CUE 1PROSITE-ProRule annotationAdd BLAST43
Domaini253 – 296CUE 2PROSITE-ProRule annotationAdd BLAST44
Domaini511 – 679Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini860 – 1012Helicase C-terminalPROSITE-ProRule annotationAdd BLAST153

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi630 – 633DEGH boxBy similarity4
Motifi723 – 740Nuclear localization signalSequence analysisAdd BLAST18
Motifi1007 – 1010DEAD boxPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 2 CUE domains.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0389. Eukaryota.
ENOG410XNUT. LUCA.
GeneTreeiENSGT00860000133865.
InParanoidiE1B7X9.
OMAiRSQNYPK.
OrthoDBiEOG091G0307.
TreeFamiTF105768.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003892. CUE.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51140. CUE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E1B7X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLFNLDRFR FEKRNKIEEA PEATPQPSQP GPSSPISLSA EEENAEGEVS
60 70 80 90 100
RAGTPDSDVT EKTEDSSVPE TPENDRKASI SYFKNQRGIQ YIDLSSDSED
110 120 130 140 150
VVSPNCSSTV QEKKFNKDTV IIVSEPSEDE ESQGLPTMAT RNNNDITNLK
160 170 180 190 200
NLSFFPNYSD NLSTVRQTRY SENLSSDLLK LIDSTSTMDG AIAAALLMFG
210 220 230 240 250
DAEGGGPRKR KLSSSSEPFE EDEFNDDQSM KKKRLDHGEE SNESAESSTN
260 270 280 290 300
WEKQESIVLK LQKEFPNFDK EELREVLKEH EWMYTEALES LKVFAEDQDM
310 320 330 340 350
QYASPSEFPN GKEVSSRSQN YPKNAAKTKL KQKCSMKPQN GFNKKRKKNV
360 370 380 390 400
FNPKRVIEDS EYDSGSDVGS SLDEDYSSGE EVMEDGYKGK ILHFLQDASI
410 420 430 440 450
GELTLIPQCS QKKAQKITEL RPFNSWEALF TKMSKTNGLS EDLIWHCKTL
460 470 480 490 500
IQERDVVIKL MNKCEDISNK LTKQVTMLTG NGGGWNTEQP SILNQSLSLK
510 520 530 540 550
PYQKVGLNWL ALVHKHGLNG ILADEMGLGK TIQAIAFLAY LYQEGNKGPH
560 570 580 590 600
LIVVPASTID NWLREVNLWC PTLKVLCYYG SQEERKQIRY NIHSRYEEYN
610 620 630 640 650
VIVTTYNCAI SSSDDRSLFR RLKLNYAIFD EGHMLKNMGS IRYQHLMTIN
660 670 680 690 700
ANNRLLLTGT PVQNNLLELM SLLNFVMPHM FSSSTSEIRR MFSSKTKPAD
710 720 730 740 750
EQSIYEKERI AHAKQIIKPF ILRRVKEEVL KQLPPKKDRI ELCAMSEKQE
760 770 780 790 800
QLYMNLFNRL KKSINNMEKN TEMCNVMMQL RKMANHPLLH RQYYTAEKLK
810 820 830 840 850
EMSQLMLKEP THCEANPDLI FEDMEVMTDF ELHVLCKQYR HINNFQLDMD
860 870 880 890 900
LILDSGKFRV LGCILSELKQ KGDRVVLFSQ FTMMLDILEV LLKHHQHRYL
910 920 930 940 950
RLDGKTQISE RIHLIDEFNT DMDIFVFLLS TKAGGLGINL TSANVVILHD
960 970 980 990 1000
IDCNPYNDKQ AEDRCHRVGQ TKEVLVIKLI GQGTIEESML KINQQKLKLE
1010 1020
QDMTTVDEGD EGSMPADIAT LLKTSMGL
Length:1,028
Mass (Da):117,573
Last modified:November 16, 2011 - v2
Checksum:iF6187DAB8C917A30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02016925 Genomic DNA. No translation available.
DAAA02016926 Genomic DNA. No translation available.
UniGeneiBt.77636.

Genome annotation databases

EnsembliENSBTAT00000047936; ENSBTAP00000045084; ENSBTAG00000017061.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02016925 Genomic DNA. No translation available.
DAAA02016926 Genomic DNA. No translation available.
UniGeneiBt.77636.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000045084.

Proteomic databases

PaxDbiE1B7X9.
PRIDEiE1B7X9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000047936; ENSBTAP00000045084; ENSBTAG00000017061.

Phylogenomic databases

eggNOGiKOG0389. Eukaryota.
ENOG410XNUT. LUCA.
GeneTreeiENSGT00860000133865.
InParanoidiE1B7X9.
OMAiRSQNYPK.
OrthoDBiEOG091G0307.
TreeFamiTF105768.

Gene expression databases

BgeeiENSBTAG00000017061.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003892. CUE.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51140. CUE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMRCD_BOVIN
AccessioniPrimary (citable) accession number: E1B7X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: November 16, 2011
Last modified: November 30, 2016
This is version 37 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.