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Protein

Disease resistance protein RRS1

Gene

RRS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Acts also as a disease resistance protein involved in resistance to fungal and bacterial pathogens, including R.solanacearum, P.syringae pv. tomato and C.higginsianum. RRS1 mediated resistance depends on salicylic acid and NDR1 (AC O48915).By similarityCurated1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi179 – 1868ATPPROSITE-ProRule annotation
DNA bindingi1204 – 127269WRKYPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Disease resistance protein RRS11 Publication
Alternative name(s):
Disease resistance protein RCH2
Disease resistance protein SLH1
Probable WRKY transcription factor 52
Protein RPS4-homolog
Protein SENSITIVE TO LOW HUMIDITY 11 Publication
Resistance to Colletotrichum higginsianum 2 protein
Resistance to Ralstonia solanacearum 1 protein1 Publication
WRKY DNA-binding protein 52
Gene namesi
Name:RRS11 Publication
Synonyms:RCH2, RRS1-R1 Publication, RSH4, SLH11 Publication, WRKY52
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1224 – 12241L → LL in slh1; loss of DNA-binding activity and constitutive defense activation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13781378Disease resistance protein RRS1PRO_0000431360Add
BLAST

Proteomic databases

PaxDbiE1B328.

Interactioni

Subunit structurei

Interacts with PopP2, a R.solanacearum type III effector.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G45260.1.

Structurei

3D structure databases

ProteinModelPortaliE1B328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 145138TIRCuratedAdd
BLAST
Domaini170 – 421252NB-ARCSequence analysisAdd
BLAST
Repeati498 – 52225LRR 1Sequence analysisAdd
BLAST
Repeati535 – 55319LRR 2Sequence analysisAdd
BLAST
Repeati554 – 57522LRR 3Sequence analysisAdd
BLAST
Repeati577 – 59822LRR 4Sequence analysisAdd
BLAST
Repeati621 – 64626LRR 5Sequence analysisAdd
BLAST
Repeati665 – 68824LRR 6Sequence analysisAdd
BLAST
Repeati742 – 76625LRR 7Sequence analysisAdd
BLAST
Repeati768 – 79326LRR 8Sequence analysisAdd
BLAST
Repeati831 – 85424LRR 9Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi988 – 100518Nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi463 – 4664Poly-ArgSequence analysis

Sequence similaritiesi

Contains 9 LRR (leucine-rich) repeats.Sequence analysis
Contains 1 NB-ARC domain.Sequence analysis
Contains 1 TIR domain.Curated
Contains 1 WRKY DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiENOG410JQXQ. Eukaryota.
ENOG410ZRAD. LUCA.
HOGENOMiHOG000115011.

Family and domain databases

Gene3Di2.20.25.80. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR011713. Leu-rich_rpt_3.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR000157. TIR_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR003657. WRKY_dom.
[Graphical view]
PfamiPF07725. LRR_3. 1 hit.
PF00931. NB-ARC. 1 hit.
PF03106. WRKY. 1 hit.
[Graphical view]
SMARTiSM00774. WRKY. 1 hit.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 1 hit.
SSF46785. SSF46785. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF52200. SSF52200. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50811. WRKY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E1B328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNCEKDEEF VCISCVEEVR YSFVSHLSEA LRRKGINNVV VDVDIDDLLF
60 70 80 90 100
KESQAKIEKA GVSVMVLPGN CDPSEVWLDK FAKVLECQRN NKDQAVVSVL
110 120 130 140 150
YGDSLLRDQW LSELDFRGLS RIHQSRKECS DSILVEEIVR DVYETHFYVG
160 170 180 190 200
RIGIYSKLLE IENMVNKQPI GIRCVGIWGM PGIGKTTLAK AVFDQMSSAF
210 220 230 240 250
DASCFIEDYD KSIHEKGLYC LLEEQLLPGN DATIMKLSSL RDRLNSKRVL
260 270 280 290 300
VVLDDVCNAL VAESFLEGFD WLGPGSLIII TSRDKQVFRL CGINQIYEVQ
310 320 330 340 350
GLNEKEARQL FLLSASIMED MGEQNLHELS VRVISYANGN PLAISVYGRE
360 370 380 390 400
LKGKKKLSEM ETAFLKLKRR PPFKIVDAFK SSYDTLSDNE KNIFLDIACF
410 420 430 440 450
FQGENVNYVI QLLEGCGFFP HVEIDVLVDK CLVTISENRV WLHKLTQDIG
460 470 480 490 500
REIINGETVQ IERRRRLWEP WSIKYLLEYN EHKANGEPKT TFKRAQGSEE
510 520 530 540 550
IEGLFLDTSN LRFDLQPSAF KNMLNLRLLK IYCSNPEVHP VINFPTGSLH
560 570 580 590 600
SLPNELRLLH WENYPLKSLP QNFDPRHLVE INMPYSQLQK LWGGTKNLEM
610 620 630 640 650
LRTIRLCHSQ HLVDIDDLLK AENLEVIDLQ GCTRLQNFPA AGRLLRLRVV
660 670 680 690 700
NLSGCIKIKS VLEIPPNIEK LHLQGTGILA LPVSTVKPNH RELVNFLTEI
710 720 730 740 750
PGLSEASKLE RLTSLLESNS SCQDLGKLIC LELKDCSCLQ SLPNMANLDL
760 770 780 790 800
NVLDLSGCSS LNSIQGFPRF LKQLYLGGTA IREVPQLPQS LEILNAHGSC
810 820 830 840 850
LRSLPNMANL EFLKVLDLSG CSELETIQGF PRNLKELYFA GTTLREVPQL
860 870 880 890 900
PLSLEVLNAH GSDSEKLPMH YKFNNFFDLS QQVVNDFFLK ALTYVKHIPR
910 920 930 940 950
GYTQELINKA PTFSFSAPSH TNQNATFDLQ PGSSVMTRLN HSWRNTLVGF
960 970 980 990 1000
GMLVEVAFPE DYCDATDVGI SCVCRWSNKE GRSCRIERNF HCWAPGKVVP
1010 1020 1030 1040 1050
KVRKDHTFVF SDVNMRPSTG EGNDPDIWAG LVVFEFFPIN QQTKCLNDRF
1060 1070 1080 1090 1100
TVTRCGVRVI NVATGNTSLE NISLVLSLDP VEVSGYEVLR VSYDDLQEMD
1110 1120 1130 1140 1150
KVLFLYIASL FNDEDVDFVA PLIAGIDLDV SSGLKVLADV SLISVSSNGE
1160 1170 1180 1190 1200
IVMHSLQRQM GKEILHGQSM LLSDCESSMT ENLSDVPKKE KKHRESKVKK
1210 1220 1230 1240 1250
VVSIPAIDEG DLWTWRKYGQ KDILGSRFPR GYYRCAYKFT HGCKATKQVQ
1260 1270 1280 1290 1300
RSETDSNMLA ITYLSEHNHP RPTKRKALAD STRSTSSSIC SAITTSASSR
1310 1320 1330 1340 1350
VFQNKDEPNQ PHLPSSSTPP RNAAVLFKMT DMEEFQDNME VDNDVVDTRT
1360 1370
LALFPEFQHQ PEEEDPWSTF FDDYNFYF
Length:1,378
Mass (Da):156,108
Last modified:November 2, 2010 - v1
Checksum:iDB20F541F0BB73F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ170631 mRNA. Translation: ADM88042.1.
AX103687 Unassigned DNA. No translation available.
AX103688 Unassigned DNA. No translation available.
AX103691 Unassigned DNA. No translation available.
AB188827 Genomic DNA. Translation: BAD38678.1.
UniGeneiAt.30021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ170631 mRNA. Translation: ADM88042.1.
AX103687 Unassigned DNA. No translation available.
AX103688 Unassigned DNA. No translation available.
AX103691 Unassigned DNA. No translation available.
AB188827 Genomic DNA. Translation: BAD38678.1.
UniGeneiAt.30021.

3D structure databases

ProteinModelPortaliE1B328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G45260.1.

Proteomic databases

PaxDbiE1B328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410JQXQ. Eukaryota.
ENOG410ZRAD. LUCA.
HOGENOMiHOG000115011.

Family and domain databases

Gene3Di2.20.25.80. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR011713. Leu-rich_rpt_3.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR000157. TIR_dom.
IPR011991. WHTH_DNA-bd_dom.
IPR003657. WRKY_dom.
[Graphical view]
PfamiPF07725. LRR_3. 1 hit.
PF00931. NB-ARC. 1 hit.
PF03106. WRKY. 1 hit.
[Graphical view]
SMARTiSM00774. WRKY. 1 hit.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 1 hit.
SSF46785. SSF46785. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF52200. SSF52200. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50811. WRKY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Resistance to Ralstonia solanacearum in Arabidopsis thaliana is conferred by the recessive RRS1-R gene, a member of a novel family of resistance genes."
    Deslandes L., Olivier J., Theulieres F., Hirsch J., Feng D.X., Bittner-Eddy P., Beynon J., Marco Y.
    Proc. Natl. Acad. Sci. U.S.A. 99:2404-2409(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION.
    Strain: cv. Nd-1.
  2. "A single amino acid insertion in the WRKY domain of the Arabidopsis TIR-NBS-LRR-WRKY-type disease resistance protein SLH1 (sensitive to low humidity 1) causes activation of defense responses and hypersensitive cell death."
    Noutoshi Y., Ito T., Seki M., Nakashita H., Yoshida S., Marco Y., Shirasu K., Shinozaki K.
    Plant J. 43:873-888(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LEU-1224, FUNCTION.
    Strain: cv. No-0.
  3. "Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus."
    Deslandes L., Olivier J., Peeters N., Feng D.X., Khounlotham M., Boucher C., Somssich I., Genin S., Marco Y.
    Proc. Natl. Acad. Sci. U.S.A. 100:8024-8029(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POPP2, SUBCELLULAR LOCATION.
    Strain: cv. Nd-1.

Entry informationi

Entry nameiWR52N_ARATH
AccessioniPrimary (citable) accession number: E1B328
Secondary accession number(s): Q0WWA0
, Q689Y9, Q8GZ83, Q9FH83, Q9FH84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 26, 2014
Last sequence update: November 2, 2010
Last modified: January 20, 2016
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Ecotypes susceptible to C.higginsianum or R.solanacearum, such as cv. Columbia, contain a protein with a premature stop codon while the longer allele found in cv. Nd-1, cv. Wassilewskija or cv. RLD confers resistance.Curated
The slh1-induced phenotype (severely stunted growth when grown under conditions of low humidity) is suppressed under conditions of high humidity and high temperature.1 Publication

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.