E0U7N5 (E0U7N5_CYAP2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
EC=2.4.1.18 HAMAP-Rule MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP-Rule MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP-Rule MF_00685
Glycogen branching enzyme HAMAP-Rule MF_00685

Gene names

Name:	glgB2 HAMAP-Rule MF_00685
Synonyms:	glgB HAMAP-Rule MF_00685
Ordered Locus Names:	Cyan7822_2889 EMBL ADN14847.1

Organism

Cyanothece sp. (strain PCC 7822) [Complete proteome] [HAMAP] EMBL ADN14847.1

Taxonomic identifier

497965 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Cyanothece

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685 SAAS SAAS006048
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685 SAAS SAAS006048
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP-Rule MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP-Rule MF_00685 SAAS SAAS006048 Glycogen metabolism HAMAP-Rule MF_00685
Molecular function	Glycosyltransferase HAMAP-Rule MF_00685 SAAS SAAS006048 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	433	1	Nucleophile By similarity HAMAP-Rule MF_00685
	Active site	486	1	Proton donor By similarity HAMAP-Rule MF_00685

Sequences

	Sequence		Length	Mass (Da)
	E0U7N5 [UniParc]. Last modified November 2, 2010. Version 1. Checksum: EE8F8F06BC7B67D0	FASTA	767	89,289
	10 20 30 40 50 60 MSTTISPEQV NQIVYNLHHD PFEILGGHPV EDNGKIKQWV VRAYLPKAQE AWVVFPTDRK 70 80 90 100 110 120 EYPMQAVHHP NFFECLIDTP ELANYQLRIR SGEHEQVIHD PYAFRSAILT DFDIHLFGEG 130 140 150 160 170 180 NHHRIYEKLG SHPVEIDGVK GVYFAVWAPN ARNVSVLGDF NQWDGRLHQM RKRDNSVWEL 190 200 210 220 230 240 FIPDIGVGSS YKYEIKNWEG HIYEKSDPYG FQQEVRPKTA SIVADLNSYQ WHDDEWLEKR 250 260 270 280 290 300 RHSNPLSQPI SVYEVHLGSW LHASSAEKTQ LLSGESEPVQ VSEWNTQARF LSYYELAHKL 310 320 330 340 350 360 IPYVKELGYT HIELLPIAEH PFDGSWGYQV TGYYAPTSRY GTPEDFMYFV DQCHQNGIGV 370 380 390 400 410 420 LVDWVPGHFP KDGHGLAFFD GTHLYEHADP RKGEHKEWGT LVFNYGRNEV RNFLVANVLF 430 440 450 460 470 480 WFDKYHIDGI RVDAVASMLY LDYARKEGEW VANEYGGREN LDAADFLRQV NSVLFGYHPG 490 500 510 520 530 540 ALSIAEESTA WPMVSWPTYM GGLGFNLKWN MGWMHDMLDY FSMDPWFRQF HQNNITFSMW 550 560 570 580 590 600 YHHSENYMLA LSHDEVVHGK SSIMGKMPGD EWQKYANVRT LFAYMFTHPG KKTMFMSMEI 610 620 630 640 650 660 GQWSEWNVWG DLEWHLLQYE PHQRLKQFFS DVNALYKSQP ALYERDFEQE GFQWVDCSDN 670 680 690 700 710 720 RHSVVSFIRR AKDPTDFVVV ICNFTPQPHS HYRIGVPESG FYTELFNSDA KKYGGSNMGN 730 740 750 760 LGGKWTDEWS FHGLPYSLDL CLPPLAVLVL KLDRDKTAEA KQQSQSE

References

[1]

"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria." Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B. MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7822.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002198 Genomic DNA. Translation: ADN14847.1.
RefSeq	YP_003888122.1. NC_014501.1.
3D structure databases
ProteinModelPortal	E0U7N5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADN14847; ADN14847; Cyan7822_2889.
GeneID	9739378.
KEGG	cyj:Cyan7822_2889.
PATRIC	42286547. VBICyaSp18455_3021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000283037.
KO	K00700.
OMA	LRAYYAY.
Enzyme and pathway databases
BioCyc	CSP497965:GJAC-2925-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

E0U7N5_CYAP2

Accession

Primary (citable) accession number: E0U7N5

Entry history

Integrated into UniProtKB/TrEMBL:	November 2, 2010
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)