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Protein

Protein translocase subunit SecA

Gene

secA

Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: May bind 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi825 – 8251ZincUniRule annotation
Metal bindingi827 – 8271ZincUniRule annotation
Metal bindingi836 – 8361ZincUniRule annotation
Metal bindingi837 – 8371ZincUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 1078ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. intracellular protein transmembrane transport Source: UniProtKB-HAMAP
  2. protein import Source: InterPro
  3. protein targeting Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Protein transport, TranslocationUniRule annotation, Transport

Keywords - Ligandi

ATP-bindingUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBSUB655816:GCOR-3538-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecAUniRule annotation
Gene namesi
Name:secAUniRule annotationImported
Ordered Locus Names:BSUW23_17300Imported
OrganismiBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)Imported
Taxonomic identifieri655816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002233 Componenti: Chromosome

Subcellular locationi

  1. Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  2. Cytoplasm UniRule annotation

  3. Note: Distribution is 50-50.UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membraneUniRule annotationSAAS annotation, CytoplasmUniRule annotationSAAS annotation, Membrane

Interactioni

Subunit structurei

Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the SecA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218169.
KOiK03070.
OMAiERFFILQ.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA.
InterProiIPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR004027. SEC_C_motif.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF02810. SEC-C. 1 hit.
PF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 2 hits.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E0U490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGILNKMFD PTKRTLNRYE RIANDIDAVR GDYENLSDDA LKHKTIEFKE
60 70 80 90 100
RLEKGATTDD LLVEAFAVVR EASRRVTGMF PFKVQLMGGV ALHDGNIAEM
110 120 130 140 150
KTGEGKTLTS TLPVYLNALT GKGVHIVTVN EYLASRDAEQ MGKIFEFLGM
160 170 180 190 200
TVGLNLNSMS KDEKREAYAA DITYSTNNEL GFDYLRDNMV LYKEQMVQRP
210 220 230 240 250
LHFAVIDEVD SILIDEARTP LIISGQAAKS TKLYVQANAF VRTLKAEKDY
260 270 280 290 300
TYDIKTKGVQ LTEEGMTKAE KAFGIDNLFD VKHVALNHHI NQSLKAHAAM
310 320 330 340 350
QKDVDYVVED GQVVIVDSFT GRLMKGRRYS EGLHQAIEAK EGLEIQNESM
360 370 380 390 400
TLATITFQNY FRMYEKLAGM TGTAKTEEEE FRNIYNMQVV TIPTNKPVVR
410 420 430 440 450
DDRPDLIYRT MEGKFKAVAE DVAQRYMTGQ PVLVGTVAVE TSELISKLLK
460 470 480 490 500
NKGIPHQVLN AKNHEREAQI IEEAGQKGAV TIATNMAGRG TDIKLGEGVK
510 520 530 540 550
ELGGLAVVGT ERHESRRIDN QLRGRSGRQG DPGITQFYLS MEDELMRRFG
560 570 580 590 600
AERTMAMLDR FGMDDSTPIQ SKMVSRAVES SQKRVEGNNF DSRKQLLQYD
610 620 630 640 650
DVLRQQREVI YKQRFEVIDS ENLREIVENM IKSSLERAIA AYTPREELPE
660 670 680 690 700
EWKLDGLVEL VNTTYLDEGA LEKSDIFGKE PDEMHELIMD RIMTKYNEKE
710 720 730 740 750
VKFGEEQMRE FEKVIVLRAV DSKWMDHIDA MDQLRQGIHL RAYAQTNPLR
760 770 780 790 800
EYQMEGFTMF ENMIESIEDE VAKFVMKAEI ENNLEREEVV QGQTTAHQQQ
810 820 830 840
EGDDNKKAKK APVRKVVDIG RNAPCHCGSG KKYKNCCGRT E
Length:841
Mass (Da):95,574
Last modified:November 2, 2010 - v1
Checksum:i62647C2588EC83A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM39495.1.
RefSeqiYP_003867804.1. NC_014479.1.

Genome annotation databases

EnsemblBacteriaiADM39495; ADM39495; BSUW23_17300.
KEGGibss:BSUW23_17300.
PATRICi42193173. VBIBacSub57968_3533.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM39495.1.
RefSeqiYP_003867804.1. NC_014479.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADM39495; ADM39495; BSUW23_17300.
KEGGibss:BSUW23_17300.
PATRICi42193173. VBIBacSub57968_3533.

Phylogenomic databases

HOGENOMiHOG000218169.
KOiK03070.
OMAiERFFILQ.

Enzyme and pathway databases

BioCyciBSUB655816:GCOR-3538-MONOMER.

Family and domain databases

Gene3Di1.10.3060.10. 1 hit.
3.40.50.300. 3 hits.
3.90.1440.10. 1 hit.
HAMAPiMF_01382. SecA.
InterProiIPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR004027. SEC_C_motif.
IPR000185. SecA.
IPR020937. SecA_CS.
IPR011115. SecA_DEAD.
IPR014018. SecA_motor_DEAD.
IPR011130. SecA_preprotein_X-link_dom.
IPR011116. SecA_Wing/Scaffold.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF02810. SEC-C. 1 hit.
PF07517. SecA_DEAD. 1 hit.
PF01043. SecA_PP_bind. 1 hit.
PF07516. SecA_SW. 1 hit.
[Graphical view]
PRINTSiPR00906. SECA.
SMARTiSM00957. SecA_DEAD. 1 hit.
SM00958. SecA_PP_bind. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
SSF81767. SSF81767. 1 hit.
SSF81886. SSF81886. 2 hits.
TIGRFAMsiTIGR00963. secA. 1 hit.
PROSITEiPS01312. SECA. 1 hit.
PS51196. SECA_MOTOR_DEAD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Bacillus subtilis subsp. spizizenii str. W23."
    Zeigler D.R.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23059 / NRRL B-14472 / W23Imported.
  2. Zeigler D.R.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: W23.

Entry informationi

Entry nameiE0U490_BACPZ
AccessioniPrimary (citable) accession number: E0U490
Entry historyi
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: April 29, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.