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Protein

Bifunctional ligase/repressor BirA

Gene

birA

Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor.UniRule annotation

Catalytic activityi

ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118BiotinUniRule annotation1
Binding sitei189BiotinUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi23 – 42H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Ligase, Repressor
Biological processTranscription, Transcription regulation
LigandATP-binding, Biotin, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ligase/repressor BirAUniRule annotation
Alternative name(s):
Biotin--[acetyl-CoA-carboxylase] ligaseUniRule annotation (EC:6.3.4.15UniRule annotation)
Biotin--protein ligaseUniRule annotation
Biotin-[acetyl-CoA carboxylase] synthetaseUniRule annotation
Gene namesi
Name:birAUniRule annotation
Ordered Locus Names:BSUW23_11005
OrganismiBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Taxonomic identifieri655816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002233 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004036601 – 325Bifunctional ligase/repressor BirAAdd BLAST325

Structurei

3D structure databases

ProteinModelPortaliE0U174
SMRiE0U174
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 262BPL/LPL catalyticPROSITE-ProRule annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 124Biotin bindingUniRule annotation3

Sequence similaritiesi

Belongs to the biotin--protein ligase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000041811
KOiK03524
OMAiRAAVWKH

Family and domain databases

CDDicd16442 BPL, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00978 Bifunct_BirA, 1 hit
InterProiView protein in InterPro
IPR030855 Bifunct_BirA
IPR004408 Biotin_CoA_COase_ligase
IPR004409 Biotin_operon_repress_HTH
IPR003142 BPL_C
IPR004143 BPL_LPL_catalytic
IPR013196 HTH_11
IPR008988 Transcriptional_repressor_C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12835:SF5 PTHR12835:SF5, 1 hit
PfamiView protein in Pfam
PF02237 BPL_C, 1 hit
PF03099 BPL_LplA_LipB, 1 hit
PF08279 HTH_11, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF50037 SSF50037, 1 hit
TIGRFAMsiTIGR00121 birA_ligase, 1 hit
TIGR00122 birA_repr_reg, 1 hit
PROSITEiView protein in PROSITE
PS51733 BPL_LPL_CATALYTIC, 1 hit

Sequencei

Sequence statusi: Complete.

E0U174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSTLRKDLI ELFSQAGSEF ISGQKISDAL GCSRTAVWKH IEELRKEGYE
60 70 80 90 100
VEAVRRKGYR LIKKPGKLSE SEIRFGLKTE VMGQHLIYQD VISSTQKTAH
110 120 130 140 150
ELANNNAPEG TLVVADKQTA GRGRMSRVWH SQEGNGIWMS LILRPDIPLQ
160 170 180 190 200
KTPQLTLLAA VAVVQGIEAA AGIQTDIKWP NDILINGKKT VGILTEMQAE
210 220 230 240 250
EDRVRSVIIG IGINVNQQSD DFPDELKDIA TSLSQASGEK IDRAGVIQHI
260 270 280 290 300
LLCFEKRYRD YMTHGFTPIK LLWESYALGI GTNMRARTLN GTFYGKALGI
310 320
DDEGVLLLET QEGIKKIYSA DIELG
Length:325
Mass (Da):36,123
Last modified:November 2, 2010 - v1
Checksum:i8766A1EF8DD9D572
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20445 Genomic DNA Translation: AAB60184.1
CP002183 Genomic DNA Translation: ADM38241.1
RefSeqiWP_003225583.1, NC_014479.1

Genome annotation databases

EnsemblBacteriaiADM38241; ADM38241; BSUW23_11005
KEGGibss:BSUW23_11005

Similar proteinsi

Entry informationi

Entry nameiBIRA_BACPZ
AccessioniPrimary (citable) accession number: E0U174
Secondary accession number(s): P42975
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 2, 2010
Last modified: April 25, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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