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E0U0H8 (TYSY2_BACPZ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase 2

Short name=TS 2
Short name=TSase 2
EC=2.1.1.45
Alternative name(s):
Thymidylate synthase B
Short name=TS B
Short name=TSase B
Gene names
Name:thyA2
Synonyms:thyB
Ordered Locus Names:BSUW23_10675
OrganismBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) [Complete proteome] [HAMAP]
Taxonomic identifier655816 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Thymidylate synthase 2 HAMAP-Rule MF_00008
PRO_0000403665

Sites

Active site1461 By similarity

Sequences

Sequence LengthMass (Da)Tools
E0U0H8 [UniParc].

Last modified November 2, 2010. Version 1.
Checksum: D121522377066038

FASTA26430,504
        10         20         30         40         50         60 
MKQYKDLCRH VLEHGEKKGD RTGTGTISTF GYQMRFHLQE GFPMLTTKKL HFKSIAHELL 

        70         80         90        100        110        120 
WFLKGDTNVR YLQENGVRIW NEWADENGEL GPVYGSQWRS WRGADGETID QISRLIEDIK 

       130        140        150        160        170        180 
TNPNSRRLIV SAWNVGEIDK MALPPCHCLF QFYVSDGKLS CQLYQRSADV FLGVPFNIAS 

       190        200        210        220        230        240 
YALLTMMIAH VTGLEPGEFI HTFGDVHIYQ NHIEQVNLQL TRDVRPLPKL RFAREIDSIF 

       250        260 
NFAFEDFIIE DYDPHPHIKG AVSV 

« Hide

References

[1]"Complete genome sequence of Bacillus subtilis subsp. spizizenii str. W23."
Zeigler D.R.
Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23059 / NRRL B-14472 / W23.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002183 Genomic DNA. Translation: ADM38175.1.
RefSeqYP_003866484.1. NC_014479.1.

3D structure databases

ProteinModelPortalE0U0H8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADM38175; ADM38175; BSUW23_10675.
GeneID9723117.
KEGGbss:BSUW23_10675.
PATRIC42190405. VBIBacSub57968_2176.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000257899.
KOK00560.
OMANLGPVYG.

Enzyme and pathway databases

BioCycBSUB655816:GCOR-2187-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY2_BACPZ
AccessionPrimary (citable) accession number: E0U0H8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 2, 2010
Last modified: February 19, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways