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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Nucleophile; for GATase activityBy similarity
Active sitei595 – 5951For Fru-6P isomerization activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

BioCyciBSUB655816:GCOR-217-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Gene namesi
Name:glmS
Ordered Locus Names:BSUW23_00920
OrganismiBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Taxonomic identifieri655816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002233 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 600599Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000403658Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliE0U070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 217216Glutamine amidotransferase type-2Add
BLAST
Domaini283 – 422140SIS 1Add
BLAST
Domaini452 – 590139SIS 2Add
BLAST

Sequence similaritiesi

Contains 2 SIS domains.Curated

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

HOGENOMiHOG000258896.
KOiK00820.
OMAiSEFRYAP.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E0U070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIVGYIGQ LDAKEILLKG LEKLEYRGYD SAGIAVANEQ GIHVFKEKGR
60 70 80 90 100
IADLREVVDA NIEAKAGIGH TRWATHGEPS YLNAHPHQSA LGRFTLVHNG
110 120 130 140 150
VIENYVQLKQ EYLQDVELKS DTDTEVVVQV IEQFVNGGLD TEEAFRKTLT
160 170 180 190 200
LLKGSYAIAL FDNENRETIF VAKNKSPLLV GLGDTFNVVA SDAMAMLQVT
210 220 230 240 250
NEYVELMDKE MVIVTDDQVV IKNLDGDVIS RASYIAELDA SDIEKGTYPH
260 270 280 290 300
YMLKETDEQP VVMRKIIQTY QDENGKLSVP GDIAAAVAEA DRIYIIGCGT
310 320 330 340 350
SYHAGLVGKQ YIEMWANVPV EVHVASEFSY NMPLLSKKPL FIFLSQSGET
360 370 380 390 400
ADSRAVLVQV KALGHKALTI TNVPGSTLSR EADYTLLLHA GPEIAVASTK
410 420 430 440 450
AYTAQIAVLA VLASVAADKN GIDIGFDLVK ELGIAANAME ALCDQKDEME
460 470 480 490 500
MIAREYLTVS RNAFFIGRGL DYFVCVEGAL KLKEISYIQA EGFAGGELKH
510 520 530 540 550
GTIALIEQGT PVFALATQEH VNLSIRGNVK EVAARGANTC IISLKGLDDA
560 570 580 590 600
DDRFVLPEVN PALAPLVSVV PLQLIAYYAA LHRGCDVDKP RNLAKSVTVE
Length:600
Mass (Da):65,339
Last modified:November 2, 2010 - v1
Checksum:i77E64F17C7E68919
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM36241.1.
D21199 Genomic DNA. Translation: BAA04741.1.
RefSeqiWP_003223659.1. NC_014479.1.
YP_003864551.1. NC_014479.1.

Genome annotation databases

EnsemblBacteriaiADM36241; ADM36241; BSUW23_00920.
KEGGibss:BSUW23_00920.
PATRICi42186358. VBIBacSub57968_0187.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM36241.1.
D21199 Genomic DNA. Translation: BAA04741.1.
RefSeqiWP_003223659.1. NC_014479.1.
YP_003864551.1. NC_014479.1.

3D structure databases

ProteinModelPortaliE0U070.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADM36241; ADM36241; BSUW23_00920.
KEGGibss:BSUW23_00920.
PATRICi42186358. VBIBacSub57968_0187.

Phylogenomic databases

HOGENOMiHOG000258896.
KOiK00820.
OMAiSEFRYAP.

Enzyme and pathway databases

BioCyciBSUB655816:GCOR-217-MONOMER.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS.
InterProiIPR017932. GATase_2_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiPF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Bacillus subtilis subsp. spizizenii str. W23."
    Zeigler D.R.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23059 / NRRL B-14472 / W23.
  2. "Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon."
    Morohoshi F., Hayashi K., Munakata N.
    J. Bacteriol. 175:6010-6017(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 471-600.
    Strain: ATCC 23059 / NRRL B-14472 / W23.

Entry informationi

Entry nameiGLMS_BACPZ
AccessioniPrimary (citable) accession number: E0U070
Secondary accession number(s): P39754
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 2, 2010
Last modified: May 27, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.