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Protein

Thymidylate synthase 1

Gene

thyA1

Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161NucleophileUniRule annotation1
Binding sitei1845,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei192dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 142dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi181 – 184dUMPUniRule annotation4
Nucleotide bindingi222 – 224dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase 1UniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TS 1UniRule annotation
Short name:
TSase 1UniRule annotation
Alternative name(s):
Thymidylate synthase A1 Publication
Short name:
TS A1 Publication
Short name:
TSase A1 Publication
Gene namesi
Name:thyA1UniRule annotation
Synonyms:thyA
Ordered Locus Names:BSUW23_09080
OrganismiBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Taxonomic identifieri655816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002233 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004036641 – 279Thymidylate synthase 1Add BLAST279

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

SMRiE0TVT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257898
KOiK00560
OMAiWNEWEVG

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

E0TVT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQFDKQYNS IIKDIMNNGI SDEEFNVRTK WDSDGTPAHT LSVISKQMRF
60 70 80 90 100
DNSEVPILTT KKVAWKTAIK ELLWIWQLKS NDVTELNKMG VHIWDQWKQE
110 120 130 140 150
DGTIGHAYGF QLGKKNRNLN GEKVDQVDYL LHQLKNNPSS RRHITMLWNP
160 170 180 190 200
DELDSMALTP CVYETQWYVK QGKLHLEVRA RSNDMALGNP FNVFQYNVLQ
210 220 230 240 250
RMIAQVTGYE LGEYIFNIGD CHVYTRHIDN LKIQMEREQF EAPELWINPE
260 270
VKDFYDFTID DFKLFNYKHG DKLLFEVAV
Length:279
Mass (Da):32,890
Last modified:November 2, 2010 - v1
Checksum:i9E808B8519B9E33C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149N → S in AAC26325 (PubMed:9648749).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA Translation: ADM37862.1
AF004103 Genomic DNA Translation: AAC26325.1
PIRiI40494
RefSeqiWP_003221167.1, NC_014479.1

Genome annotation databases

EnsemblBacteriaiADM37862; ADM37862; BSUW23_09080
KEGGibss:BSUW23_09080

Similar proteinsi

Entry informationi

Entry nameiTYSY1_BACPZ
AccessioniPrimary (citable) accession number: E0TVT6
Secondary accession number(s): O30395, O30396, P42326
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 2, 2010
Last modified: March 28, 2018
This is version 42 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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