Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidylate synthase 1

Gene

thyA1

Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161NucleophileUniRule annotation1
Binding sitei1845,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei192dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 142dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi181 – 184dUMPUniRule annotation4
Nucleotide bindingi222 – 224dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase 1UniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TS 1UniRule annotation
Short name:
TSase 1UniRule annotation
Alternative name(s):
Thymidylate synthase A1 Publication
Short name:
TS A1 Publication
Short name:
TSase A1 Publication
Gene namesi
Name:thyA1UniRule annotation
Synonyms:thyA
Ordered Locus Names:BSUW23_09080
OrganismiBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Taxonomic identifieri655816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002233 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004036641 – 279Thymidylate synthase 1Add BLAST279

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257898.
KOiK00560.
OMAiFEAPELW.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E0TVT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQFDKQYNS IIKDIMNNGI SDEEFNVRTK WDSDGTPAHT LSVISKQMRF
60 70 80 90 100
DNSEVPILTT KKVAWKTAIK ELLWIWQLKS NDVTELNKMG VHIWDQWKQE
110 120 130 140 150
DGTIGHAYGF QLGKKNRNLN GEKVDQVDYL LHQLKNNPSS RRHITMLWNP
160 170 180 190 200
DELDSMALTP CVYETQWYVK QGKLHLEVRA RSNDMALGNP FNVFQYNVLQ
210 220 230 240 250
RMIAQVTGYE LGEYIFNIGD CHVYTRHIDN LKIQMEREQF EAPELWINPE
260 270
VKDFYDFTID DFKLFNYKHG DKLLFEVAV
Length:279
Mass (Da):32,890
Last modified:November 2, 2010 - v1
Checksum:i9E808B8519B9E33C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149N → S in AAC26325 (PubMed:9648749).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM37862.1.
AF004103 Genomic DNA. Translation: AAC26325.1.
PIRiI40494.
RefSeqiWP_003221167.1. NC_014479.1.

Genome annotation databases

EnsemblBacteriaiADM37862; ADM37862; BSUW23_09080.
KEGGibss:BSUW23_09080.
PATRICi42189751. VBIBacSub57968_1850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM37862.1.
AF004103 Genomic DNA. Translation: AAC26325.1.
PIRiI40494.
RefSeqiWP_003221167.1. NC_014479.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADM37862; ADM37862; BSUW23_09080.
KEGGibss:BSUW23_09080.
PATRICi42189751. VBIBacSub57968_1850.

Phylogenomic databases

HOGENOMiHOG000257898.
KOiK00560.
OMAiFEAPELW.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY1_BACPZ
AccessioniPrimary (citable) accession number: E0TVT6
Secondary accession number(s): O30395, O30396, P42326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 2, 2010
Last modified: November 30, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.