Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Non-canonical purine NTP pyrophosphatase

Gene

ysnA

Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+, Mn2+Note: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn2+.
  • Mg2+UniRule annotationSAAS annotation, Mn2+UniRule annotationSAAS annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn2+.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411Magnesium or manganeseUniRule annotation
Metal bindingi70 – 701Magnesium or manganeseUniRule annotation
Binding sitei156 – 1561SubstrateUniRule annotation
Binding sitei176 – 1761SubstrateUniRule annotation
Binding sitei182 – 1821SubstrateUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nucleoside-triphosphatase activity Source: InterPro
  3. nucleoside-triphosphate diphosphatase activity Source: UniProtKB-HAMAP
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleoside triphosphate catabolic process Source: InterPro
  2. purine nucleotide metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Nucleotide metabolismUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciBSUB655816:GCOR-2804-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical purine NTP pyrophosphataseUniRule annotationSAAS annotation (EC:3.6.1.19UniRule annotationSAAS annotation)
Alternative name(s):
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Gene namesi
Name:ysnAImported
Ordered Locus Names:BSUW23_13755Imported
OrganismiBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)Imported
Taxonomic identifieri655816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002233 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 136Substrate bindingUniRule annotation
Regioni70 – 712Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

E0TVB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEAIIATHN PGKVKEFKEI LEPKGYDVKS LAEIGFTEEI EETGHTFEEN
60 70 80 90 100
AILKAQAVAK AVNKMVIADD SGLSIDNLGG RPGVYSARYA GEQKDDQANI
110 120 130 140 150
AKVLSELKGI EKEQRTARFR CALAVSIPGE ETKTVEGHVE GYIAEEPRGE
160 170 180 190
YGFGYDPIFI VKDKDKTMAE LTSDEKNKIS HRADALKKLS KLLEA
Length:195
Mass (Da):21,470
Last modified:November 2, 2010 - v1
Checksum:i7DD5262967661496
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM38788.1.
RefSeqiYP_003867097.1. NC_014479.1.

Genome annotation databases

EnsemblBacteriaiADM38788; ADM38788; BSUW23_13755.
KEGGibss:BSUW23_13755.
PATRICi42191656. VBIBacSub57968_2800.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM38788.1.
RefSeqiYP_003867097.1. NC_014479.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADM38788; ADM38788; BSUW23_13755.
KEGGibss:BSUW23_13755.
PATRICi42191656. VBIBacSub57968_2800.

Phylogenomic databases

HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.

Enzyme and pathway databases

BioCyciBSUB655816:GCOR-2804-MONOMER.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Bacillus subtilis subsp. spizizenii str. W23."
    Zeigler D.R.
    Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23059 / NRRL B-14472 / W23Imported.
  2. Zeigler D.R.
    Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: W23.

Entry informationi

Entry nameiE0TVB7_BACPZ
AccessioniPrimary (citable) accession number: E0TVB7
Entry historyi
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: April 1, 2015
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.