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Protein

dITP/XTP pyrophosphatase

Gene

ysnA

Organism
Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotationSAAS annotation

Catalytic activityi

ITP + H2O = IMP + diphosphate.UniRule annotationSAAS annotation
XTP + H2O = XMP + diphosphate.UniRule annotationSAAS annotation
dITP + H2O = dIMP + diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41MagnesiumUniRule annotation1
Active sitei70Proton acceptorUniRule annotation1
Metal bindingi70MagnesiumUniRule annotation1
Binding sitei71Substrate; via amide nitrogenUniRule annotation1
Binding sitei176SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation
Biological processNucleotide metabolismUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-bindingUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Name:ysnAImported
Ordered Locus Names:BSUW23_13755Imported
OrganismiBacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23)Imported
Taxonomic identifieri655816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002233 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 13Substrate bindingUniRule annotation6
Regioni153 – 156Substrate bindingUniRule annotation4
Regioni181 – 182Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000293319.
KOiK02428.
OMAiYDPIFQP.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

E0TVB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEAIIATHN PGKVKEFKEI LEPKGYDVKS LAEIGFTEEI EETGHTFEEN
60 70 80 90 100
AILKAQAVAK AVNKMVIADD SGLSIDNLGG RPGVYSARYA GEQKDDQANI
110 120 130 140 150
AKVLSELKGI EKEQRTARFR CALAVSIPGE ETKTVEGHVE GYIAEEPRGE
160 170 180 190
YGFGYDPIFI VKDKDKTMAE LTSDEKNKIS HRADALKKLS KLLEA
Length:195
Mass (Da):21,470
Last modified:November 2, 2010 - v1
Checksum:i7DD5262967661496
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002183 Genomic DNA. Translation: ADM38788.1.

Genome annotation databases

EnsemblBacteriaiADM38788; ADM38788; BSUW23_13755.
KEGGibss:BSUW23_13755.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiE0TVB7_BACPZ
AccessioniPrimary (citable) accession number: E0TVB7
Entry historyiIntegrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: July 5, 2017
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported