Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

E0SQN9

- E0SQN9_IGNAA

UniProt

E0SQN9 - E0SQN9_IGNAA

Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 26 (01 Oct 2014)
      Sequence version 1 (02 Nov 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei34 – 341UniRule annotation
    Binding sitei37 – 371ATPUniRule annotation
    Binding sitei76 – 761ATPUniRule annotation
    Metal bindingi78 – 781Magnesium 1UniRule annotation
    Active sitei80 – 801Proton acceptorUniRule annotation
    Binding sitei101 – 1011SubstrateUniRule annotation
    Metal bindingi102 – 1021Magnesium 2UniRule annotation
    Binding sitei224 – 2241SubstrateUniRule annotation
    Metal bindingi252 – 2521Magnesium 2UniRule annotation
    Binding sitei479 – 4791ATPUniRule annotation
    Binding sitei516 – 5161ATP; via carbonyl oxygen and amide nitrogenUniRule annotation
    Binding sitei519 – 5191SubstrateUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Biological processi

    Purine biosynthesisUniRule annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciIAGG583356:GHAH-279-MONOMER.
    UniPathwayiUPA00074; UER00128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
    Glutamine amidotransferase PurLUniRule annotation
    Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
    Gene namesi
    Name:purLUniRule annotation
    Ordered Locus Names:Igag_0264Imported
    OrganismiIgnisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)Imported
    Taxonomic identifieri583356 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeIgnisphaera
    ProteomesiUP000001304: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni79 – 824Substrate bindingUniRule annotation
    Regioni295 – 2973Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the FGAMS family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000238227.
    KOiK01952.
    OMAiDHMVGTD.

    Family and domain databases

    Gene3Di3.30.1330.10. 2 hits.
    HAMAPiMF_00420. PurL_2.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR010074. PRibForGlyAmidine_synth_II.
    IPR016188. PurM_N-like.
    [Graphical view]
    PfamiPF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    E0SQN9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLTNDEIEE IKRILGREPT VEELAMFEAQ WSEHCSYKSS RLLLRLLPTK    50
    AKHVVLGPGR DAPAVEVFPD VLVVFKIESH NHPSAVDPYN GAATGIGGIV 100
    RDILTLGAKP IALLDLLYMG NPRDPHANWL IRGIVKGISD YGNRIGIPTV 150
    AGDTWFDEAF NKQPLVNVAC VGIVSPDKAV LSGPKSGDLI LIIGNATGRD 200
    GLLGSSFASR PLAEDVDKDI GAVQVGDPLT EKLLIDVIQI LVEKNLVNYI 250
    KDLGGGGLTT ALSESAADHG LGAVIYLDKL HTRQSLTPLE LLVSESQERM 300
    LLAVPPTKIE DVKNILEGYE VPYSIIGYFD DSGIIKVFYM DRKVVEAPAK 350
    ELAKPRAIRR PSKPPTEALK GFEPIILPDT EIDIEKTILL LLSSPNIASK 400
    KWIYEQYDHE VGARTIIKPG YGDAAVLRLL DGSRRGIAVK GDANPRYTYL 450
    DPFNGAANVV SECYRNLTAV GSTPLAIVDE LNAGNPEKPE HYWYFEEMLK 500
    GLAWISDELG LPIVGGKVSF YNEDSRGVQV KPTLTVVGVG RIDDISYARG 550
    MGFREYGSYI VVIGTTYPEI GGTEYLYRVH GIEHGEIPKP RPTYEISHGN 600
    IVRRLIHRDI ALSVHDIGLG GLGIALIEMA IVSGIGFDVD LSVIPMRAVD 650
    RLDVLLFSET QARYIIEIDG RRLDEAKKIL DESSVVYSVI GVTKGYEKTV 700
    FRYNGNVVAS IDLINAVDIY MNSLERELEV V 731
    Length:731
    Mass (Da):80,109
    Last modified:November 2, 2010 - v1
    Checksum:i5B2D021AB497EA0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002098 Genomic DNA. Translation: ADM27113.1.
    RefSeqiWP_013302763.1. NC_014471.1.
    YP_003858993.1. NC_014471.1.

    Genome annotation databases

    EnsemblBacteriaiADM27113; ADM27113; Igag_0264.
    GeneIDi9715580.
    KEGGiiag:Igag_0264.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002098 Genomic DNA. Translation: ADM27113.1 .
    RefSeqi WP_013302763.1. NC_014471.1.
    YP_003858993.1. NC_014471.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADM27113 ; ADM27113 ; Igag_0264 .
    GeneIDi 9715580.
    KEGGi iag:Igag_0264.

    Phylogenomic databases

    HOGENOMi HOG000238227.
    KOi K01952.
    OMAi DHMVGTD.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .
    BioCyci IAGG583356:GHAH-279-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.10. 2 hits.
    HAMAPi MF_00420. PurL_2.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR010074. PRibForGlyAmidine_synth_II.
    IPR016188. PurM_N-like.
    [Graphical view ]
    Pfami PF00586. AIRS. 2 hits.
    PF02769. AIRS_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55326. SSF55326. 2 hits.
    SSF56042. SSF56042. 2 hits.
    TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17230 / JCM 13409 / AQ1.S1Imported.

    Entry informationi

    Entry nameiE0SQN9_IGNAA
    AccessioniPrimary (citable) accession number: E0SQN9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 2, 2010
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS.UniRule annotation

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3