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E0SQN9

- E0SQN9_IGNAA

UniProt

E0SQN9 - E0SQN9_IGNAA

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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341UniRule annotation
Binding sitei37 – 371ATPUniRule annotation
Binding sitei76 – 761ATPUniRule annotation
Metal bindingi78 – 781Magnesium 1UniRule annotation
Active sitei80 – 801Proton acceptorUniRule annotation
Binding sitei101 – 1011SubstrateUniRule annotation
Metal bindingi102 – 1021Magnesium 2UniRule annotation
Binding sitei224 – 2241SubstrateUniRule annotation
Metal bindingi252 – 2521Magnesium 2UniRule annotation
Binding sitei479 – 4791ATPUniRule annotation
Binding sitei516 – 5161ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei519 – 5191SubstrateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciIAGG583356:GHAH-279-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:Igag_0264Imported
OrganismiIgnisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)Imported
Taxonomic identifieri583356 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeIgnisphaera
ProteomesiUP000001304: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 824Substrate bindingUniRule annotation
Regioni295 – 2973Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000238227.
KOiK01952.
OMAiDHMVGTD.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

E0SQN9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLTNDEIEE IKRILGREPT VEELAMFEAQ WSEHCSYKSS RLLLRLLPTK
60 70 80 90 100
AKHVVLGPGR DAPAVEVFPD VLVVFKIESH NHPSAVDPYN GAATGIGGIV
110 120 130 140 150
RDILTLGAKP IALLDLLYMG NPRDPHANWL IRGIVKGISD YGNRIGIPTV
160 170 180 190 200
AGDTWFDEAF NKQPLVNVAC VGIVSPDKAV LSGPKSGDLI LIIGNATGRD
210 220 230 240 250
GLLGSSFASR PLAEDVDKDI GAVQVGDPLT EKLLIDVIQI LVEKNLVNYI
260 270 280 290 300
KDLGGGGLTT ALSESAADHG LGAVIYLDKL HTRQSLTPLE LLVSESQERM
310 320 330 340 350
LLAVPPTKIE DVKNILEGYE VPYSIIGYFD DSGIIKVFYM DRKVVEAPAK
360 370 380 390 400
ELAKPRAIRR PSKPPTEALK GFEPIILPDT EIDIEKTILL LLSSPNIASK
410 420 430 440 450
KWIYEQYDHE VGARTIIKPG YGDAAVLRLL DGSRRGIAVK GDANPRYTYL
460 470 480 490 500
DPFNGAANVV SECYRNLTAV GSTPLAIVDE LNAGNPEKPE HYWYFEEMLK
510 520 530 540 550
GLAWISDELG LPIVGGKVSF YNEDSRGVQV KPTLTVVGVG RIDDISYARG
560 570 580 590 600
MGFREYGSYI VVIGTTYPEI GGTEYLYRVH GIEHGEIPKP RPTYEISHGN
610 620 630 640 650
IVRRLIHRDI ALSVHDIGLG GLGIALIEMA IVSGIGFDVD LSVIPMRAVD
660 670 680 690 700
RLDVLLFSET QARYIIEIDG RRLDEAKKIL DESSVVYSVI GVTKGYEKTV
710 720 730
FRYNGNVVAS IDLINAVDIY MNSLERELEV V
Length:731
Mass (Da):80,109
Last modified:November 2, 2010 - v1
Checksum:i5B2D021AB497EA0B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002098 Genomic DNA. Translation: ADM27113.1.
RefSeqiWP_013302763.1. NC_014471.1.
YP_003858993.1. NC_014471.1.

Genome annotation databases

EnsemblBacteriaiADM27113; ADM27113; Igag_0264.
GeneIDi9715580.
KEGGiiag:Igag_0264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002098 Genomic DNA. Translation: ADM27113.1 .
RefSeqi WP_013302763.1. NC_014471.1.
YP_003858993.1. NC_014471.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADM27113 ; ADM27113 ; Igag_0264 .
GeneIDi 9715580.
KEGGi iag:Igag_0264.

Phylogenomic databases

HOGENOMi HOG000238227.
KOi K01952.
OMAi DHMVGTD.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci IAGG583356:GHAH-279-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
HAMAPi MF_00420. PurL_2.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17230 / JCM 13409 / AQ1.S1Imported.

Entry informationi

Entry nameiE0SQN9_IGNAA
AccessioniPrimary (citable) accession number: E0SQN9
Entry historyi
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: October 29, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3