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E0SQN9

- E0SQN9_IGNAA

UniProt

E0SQN9 - E0SQN9_IGNAA

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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene
purL, Igag_0264
Organism
Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL By similarity.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341 By similarityUniRule annotation
Binding sitei37 – 371ATP By similarityUniRule annotation
Binding sitei76 – 761ATP By similarityUniRule annotation
Metal bindingi78 – 781Magnesium 1 By similarityUniRule annotation
Active sitei80 – 801Proton acceptor By similarityUniRule annotation
Binding sitei101 – 1011Substrate By similarityUniRule annotation
Metal bindingi102 – 1021Magnesium 2 By similarityUniRule annotation
Binding sitei224 – 2241Substrate By similarityUniRule annotation
Metal bindingi252 – 2521Magnesium 2 By similarityUniRule annotation
Binding sitei479 – 4791ATP By similarityUniRule annotation
Binding sitei516 – 5161ATP; via carbonyl oxygen and amide nitrogen By similarityUniRule annotation
Binding sitei519 – 5191Substrate By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciIAGG583356:GHAH-279-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit II
Glutamine amidotransferase PurL
Phosphoribosylformylglycinamidine synthase subunit II
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:Igag_0264Imported
OrganismiIgnisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)Imported
Taxonomic identifieri583356 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeIgnisphaera
ProteomesiUP000001304: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits By similarity.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 824Substrate binding By similarityUniRule annotation
Regioni295 – 2973Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000238227.
KOiK01952.
OMAiDHMVGTD.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

E0SQN9-1 [UniParc]FASTAAdd to Basket

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MPLTNDEIEE IKRILGREPT VEELAMFEAQ WSEHCSYKSS RLLLRLLPTK    50
AKHVVLGPGR DAPAVEVFPD VLVVFKIESH NHPSAVDPYN GAATGIGGIV 100
RDILTLGAKP IALLDLLYMG NPRDPHANWL IRGIVKGISD YGNRIGIPTV 150
AGDTWFDEAF NKQPLVNVAC VGIVSPDKAV LSGPKSGDLI LIIGNATGRD 200
GLLGSSFASR PLAEDVDKDI GAVQVGDPLT EKLLIDVIQI LVEKNLVNYI 250
KDLGGGGLTT ALSESAADHG LGAVIYLDKL HTRQSLTPLE LLVSESQERM 300
LLAVPPTKIE DVKNILEGYE VPYSIIGYFD DSGIIKVFYM DRKVVEAPAK 350
ELAKPRAIRR PSKPPTEALK GFEPIILPDT EIDIEKTILL LLSSPNIASK 400
KWIYEQYDHE VGARTIIKPG YGDAAVLRLL DGSRRGIAVK GDANPRYTYL 450
DPFNGAANVV SECYRNLTAV GSTPLAIVDE LNAGNPEKPE HYWYFEEMLK 500
GLAWISDELG LPIVGGKVSF YNEDSRGVQV KPTLTVVGVG RIDDISYARG 550
MGFREYGSYI VVIGTTYPEI GGTEYLYRVH GIEHGEIPKP RPTYEISHGN 600
IVRRLIHRDI ALSVHDIGLG GLGIALIEMA IVSGIGFDVD LSVIPMRAVD 650
RLDVLLFSET QARYIIEIDG RRLDEAKKIL DESSVVYSVI GVTKGYEKTV 700
FRYNGNVVAS IDLINAVDIY MNSLERELEV V 731
Length:731
Mass (Da):80,109
Last modified:November 2, 2010 - v1
Checksum:i5B2D021AB497EA0B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002098 Genomic DNA. Translation: ADM27113.1.
RefSeqiWP_013302763.1. NC_014471.1.
YP_003858993.1. NC_014471.1.

Genome annotation databases

EnsemblBacteriaiADM27113; ADM27113; Igag_0264.
GeneIDi9715580.
KEGGiiag:Igag_0264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002098 Genomic DNA. Translation: ADM27113.1 .
RefSeqi WP_013302763.1. NC_014471.1.
YP_003858993.1. NC_014471.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADM27113 ; ADM27113 ; Igag_0264 .
GeneIDi 9715580.
KEGGi iag:Igag_0264.

Phylogenomic databases

HOGENOMi HOG000238227.
KOi K01952.
OMAi DHMVGTD.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci IAGG583356:GHAH-279-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
HAMAPi MF_00420. PurL_2.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17230 / JCM 13409 / AQ1.S1.

Entry informationi

Entry nameiE0SQN9_IGNAA
AccessioniPrimary (citable) accession number: E0SQN9
Entry historyi
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: September 3, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS By similarity.UniRule annotation

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi