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Protein

Phosphoribosylformylglycinamidine synthase subunit PurQ

Gene

purQ

Organism
Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS), Phosphoribosylformylglycinamidine synthase subunit PurL (purL)
  2. no protein annotated in this organism
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei87NucleophileUniRule annotation1
Active sitei196UniRule annotation1
Active sitei198UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation, LigaseUniRule annotationImported
Biological processPurine biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurQUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IUniRule annotation
Short name:
FGAR amidotransferase IUniRule annotation
Short name:
FGAR-AT IUniRule annotation
Glutaminase PurQUniRule annotation (EC:3.5.1.2UniRule annotation)
Phosphoribosylformylglycinamidine synthase subunit IUniRule annotation
Gene namesi
Name:purQUniRule annotation
Ordered Locus Names:Igag_0263Imported
OrganismiIgnisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)Imported
Taxonomic identifieri583356 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiDesulfurococcalesDesulfurococcaceaeIgnisphaera
Proteomesi
  • UP000001304 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi583356.Igag_0263.

Structurei

3D structure databases

ProteinModelPortaliE0SQN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 229Glutamine amidotransferase type-1InterPro annotationAdd BLAST227

Keywords - Domaini

Glutamine amidotransferaseUniRule annotation

Phylogenomic databases

eggNOGiarCOG00102. Archaea.
COG0047. LUCA.
HOGENOMiHOG000238240.
KOiK01952.
OMAiNCDRDVA.
OrthoDBiPOG093Z0ACN.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00421. PurQ. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010075. PRibForGlyAmidine_synth_PurQ.
PIRSFiPIRSF001586. FGAM_synth_I. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01737. FGAM_synth_I. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

E0SQN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARIAVIKFP GTNCDEDVAH AINTVTSLKA EVIWYKDFTL DPWDAIVIPG
60 70 80 90 100
GFSYGDWLRA GAIAARTSVL ERIAEAVDRG IPVLGICNGF QILVEAGLLP
110 120 130 140 150
GALTVNDIGR FVCKWVKLYV ERPRGPWLSL AEDGMELDMP IAHAEGRYIV
160 170 180 190 200
DSETYNSILM KSPLIRYKDG WNSNGSLYNI AGVASEDGYV LGLMPHPERA
210 220
SEEELTPPRF SPGGRVIFKS IEYSIKRGW
Length:229
Mass (Da):25,303
Last modified:November 2, 2010 - v1
Checksum:iBA17EB35488A8218
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002098 Genomic DNA. Translation: ADM27112.1.

Genome annotation databases

EnsemblBacteriaiADM27112; ADM27112; Igag_0263.
KEGGiiag:Igag_0263.

Similar proteinsi

Entry informationi

Entry nameiE0SQN8_IGNAA
AccessioniPrimary (citable) accession number: E0SQN8
Entry historyiIntegrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: September 27, 2017
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported