ID   E0SJQ4_DICD3            Unreviewed;       343 AA.
AC   E0SJQ4;
DT   02-NOV-2010, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2010, sequence version 1.
DT   27-MAR-2024, entry version 72.
DE   SubName: Full=Gamma-aminobutyric-acid receptor subunit beta-1 {ECO:0000313|EMBL:ADN00343.1};
GN   OrderedLocusNames=Dda3937_00520 {ECO:0000313|EMBL:ADN00343.1};
OS   Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Dickeya.
OX   NCBI_TaxID=198628 {ECO:0000313|EMBL:ADN00343.1, ECO:0000313|Proteomes:UP000006859};
RN   [1] {ECO:0000313|EMBL:ADN00343.1, ECO:0000313|Proteomes:UP000006859}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=3937 {ECO:0000313|EMBL:ADN00343.1,
RC   ECO:0000313|Proteomes:UP000006859};
RX   PubMed=21217001; DOI=10.1128/JB.01513-10;
RA   Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N.,
RA   Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D.,
RA   Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K.,
RA   Rauscher L., Rodriguez-Palenzuela P., Toussaint A., Holeva M.C., He S.Y.,
RA   Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S.,
RA   Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S.,
RA   Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M.,
RA   Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R.,
RA   Blattner F.R., Keen N.T., Perna N.T.;
RT   "Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937.";
RL   J. Bacteriol. 193:2076-2077(2011).
RN   [2] {ECO:0007829|PDB:3RQU, ECO:0007829|PDB:3RQW}
RP   X-RAY CRYSTALLOGRAPHY (2.91 ANGSTROMS) OF 22-343.
RX   PubMed=22395605; DOI=10.1038/ncomms1703;
RA   Pan J., Chen Q., Willenbring D., Yoshida K., Tillman T., Kashlan O.B.,
RA   Cohen A., Kong X.P., Xu Y., Tang P.;
RT   "Structure of the pentameric ligand-gated ion channel ELIC cocrystallized
RT   with its competitive antagonist acetylcholine.";
RL   Nat. Commun. 3:714-714(2012).
RN   [3] {ECO:0007829|PDB:2YN6}
RP   X-RAY CRYSTALLOGRAPHY (3.31 ANGSTROMS) OF 22-343.
RX   PubMed=23185134; DOI=10.1371/journal.pbio.1001429;
RA   Zimmermann I., Marabelli A., Bertozzi C., Sivilotti L.G., Dutzler R.;
RT   "Inhibition of the prokaryotic pentameric ligand-gated ion channel ELIC by
RT   divalent cations.";
RL   PLoS Biol. 10:e1001429-e1001429(2012).
RN   [4] {ECO:0007829|PDB:3UQ4, ECO:0007829|PDB:3UQ5}
RP   X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) OF 22-343.
RX   PubMed=22474383; DOI=10.1073/pnas.1119268109;
RA   Gonzalez-Gutierrez G., Lukk T., Agarwal V., Papke D., Nair S.K.,
RA   Grosman C.;
RT   "Mutations that stabilize the open state of the Erwinia chrisanthemi
RT   ligand-gated ion channel fail to change the conformation of the pore domain
RT   in crystals.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:6331-6336(2012).
RN   [5] {ECO:0007829|PDB:4Z90, ECO:0007829|PDB:4Z91}
RP   X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 22-343.
RX   PubMed=26346220; DOI=10.1038/srep13833;
RA   Chen Q., Kinde M.N., Arjunan P., Wells M.M., Cohen A.E., Xu Y., Tang P.;
RT   "Direct Pore Binding as a Mechanism for Isoflurane Inhibition of the
RT   Pentameric Ligand-gated Ion Channel ELIC.";
RL   Sci. Rep. 5:13833-13833(2015).
RN   [6] {ECO:0007829|PDB:5HEJ, ECO:0007829|PDB:5HEO}
RP   X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) OF 22-343.
RX   PubMed=26943937; DOI=10.1371/journal.pbio.1002393;
RA   Bertozzi C., Zimmermann I., Engeler S., Hilf R.J., Dutzler R.;
RT   "Signal Transduction at the Domain Interface of Prokaryotic Pentameric
RT   Ligand-Gated Ion Channels.";
RL   PLoS Biol. 14:e1002393-e1002393(2016).
RN   [7] {ECO:0007829|PDB:5LG3}
RP   X-RAY CRYSTALLOGRAPHY (3.57 ANGSTROMS) OF 32-338.
RX   PubMed=27791038; DOI=10.1073/pnas.1603101113;
RA   Nys M., Wijckmans E., Farinha A., Yoluk O., Andersson M., Brams M.,
RA   Spurny R., Peigneur S., Tytgat J., Lindahl E., Ulens C.;
RT   "Allosteric binding site in a Cys-loop receptor ligand-binding domain
RT   unveiled in the crystal structure of ELIC in complex with chlorpromazine.";
RL   Proc. Natl. Acad. Sci. U.S.A. 113:E6696-E6703(2016).
RN   [8] {ECO:0007829|PDB:5SXU}
RP   X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 22-343.
RX   PubMed=27916519; DOI=10.1016/j.str.2016.11.007;
RA   Chen Q., Wells M.M., Tillman T.S., Kinde M.N., Cohen A., Xu Y., Tang P.;
RT   "Structural Basis of Alcohol Inhibition of the Pentameric Ligand-Gated Ion
RT   Channel ELIC.";
RL   Structure 25:180-187(2017).
RN   [9] {ECO:0007829|PDB:6CDU, ECO:0007829|PDB:6D1S}
RP   X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) OF 22-220.
RX   PubMed=30266951; DOI=10.1038/s41467-018-06361-4;
RA   Chen Q., Wells M.M., Arjunan P., Tillman T.S., Cohen A.E., Xu Y., Tang P.;
RT   "Structural basis of neurosteroid anesthetic action on GABAA receptors.";
RL   Nat. Commun. 9:3972-3972(2018).
RN   [10] {ECO:0007829|PDB:6V03, ECO:0007829|PDB:6V0B}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.30 ANGSTROMS) OF 22-341.
RX   PubMed=31911476; DOI=10.1073/pnas.1906823117;
RA   Kumar P., Wang Y., Zhang Z., Zhao Z., Cymes G.D., Tajkhorshid E.,
RA   Grosman C.;
RT   "Cryo-EM structures of a lipid-sensitive pentameric ligand-gated ion
RT   channel embedded in a phosphatidylcholine-only bilayer.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:1788-1798(2020).
RN   [11] {ECO:0007829|PDB:7L6Q, ECO:0007829|PDB:7L6U}
RP   STRUCTURE BY ELECTRON MICROSCOPY (2.50 ANGSTROMS) OF 22-343.
RX   PubMed=34083441; DOI=10.1073/pnas.2100164118;
RA   Kumar P., Cymes G.D., Grosman C.;
RT   "Structure and function at the lipid-protein interface of a pentameric
RT   ligand-gated ion channel. .";
RL   Proc. Natl. Acad. Sci. U.S.A. 118:e2100164118-e2100164118(2021).
RN   [12] {ECO:0007829|PDB:8D63, ECO:0007829|PDB:8D64}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.14 ANGSTROMS) OF 22-343.
RX   PubMed=36385237; DOI=10.1038/s41467-022-34813-5;
RA   Petroff J.T., Dietzen N.M., Santiago-McRae E., Deng B., Washington M.S.,
RA   Chen L.J., Trent Moreland K., Deng Z., Rau M., Fitzpatrick J.A.J., Yuan P.,
RA   Joseph T.T., Henin J., Brannigan G., Cheng W.W.L.;
RT   "Open-channel structure of a pentameric ligand-gated ion channel reveals a
RT   mechanism of leaflet-specific phospholipid modulation.";
RL   Nat. Commun. 13:7017-7017(2022).
RN   [13] {ECO:0007829|PDB:8F35}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.17 ANGSTROMS) OF 22-343.
RA   Dalal V., Arcario M.J., Petroff II J.T., Deitzen N.M., Tan B.K.,
RA   Brannigan G., Cheng W.W.L.;
RT   "Apo ELIC in spMSP1D1 nanodiscs with 2:1:1 POPC:POPE:POPG.";
RL   Submitted (NOV-2022) to the PDB data bank.
RN   [14] {ECO:0007829|PDB:8F33}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.28 ANGSTROMS) OF 22-343.
RA   Dalal V., Arcario M.J., Petroff II J.T., Deitzen N.M., Tan B.K.,
RA   Brannigan G., Cheng W.W.L.;
RT   "ELIC with Propylamine in saposin nanodiscs with 2:1:1 POPC:POPE:POPG.";
RL   Submitted (NOV-2022) to the PDB data bank.
RN   [15] {ECO:0007829|PDB:8F32}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.71 ANGSTROMS) OF 22-343.
RA   Dalal V., Arcario M.J., Petroff II J.T., Deitzen N.M., Tan B.K.,
RA   Brannigan G., Cheng W.W.L.;
RT   "ELIC with Propylamine in SMA nanodiscs with 2:1:1 POPC:POPE:POPG.";
RL   Submitted (NOV-2022) to the PDB data bank.
RN   [16] {ECO:0007829|PDB:8F34}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.12 ANGSTROMS) OF 22-343.
RA   Dalal V., Arcario M.J., Petroff II J.T., Deitzen N.M., Tan B.K.,
RA   Brannigan G., Cheng W.W.L.;
RT   "ELIC with Propylamine in spMSP1D1 nanodiscs with 2:1:1 POPC:POPE:POPG.";
RL   Submitted (NOV-2022) to the PDB data bank.
CC   -!- INTERACTION:
CC       E0SJQ4; E0SJQ4: Dda3937_00520; NbExp=4; IntAct=EBI-15977988, EBI-15977988;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR   EMBL; CP002038; ADN00343.1; -; Genomic_DNA.
DR   RefSeq; WP_013319743.1; NC_014500.1.
DR   PDB; 2YN6; X-ray; 3.31 A; A/B/C/D/E=22-343.
DR   PDB; 3RQU; X-ray; 3.09 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 3RQW; X-ray; 2.91 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 3UQ4; X-ray; 3.50 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 3UQ5; X-ray; 4.20 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 3UQ7; X-ray; 3.80 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 4Z90; X-ray; 3.00 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 4Z91; X-ray; 3.39 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 5HEJ; X-ray; 3.50 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 5HEO; X-ray; 3.30 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 5HEU; X-ray; 3.20 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 5HEW; X-ray; 4.50 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 5LG3; X-ray; 3.57 A; A/B/C/D/E/F/G/H/I/J=32-338.
DR   PDB; 5SXU; X-ray; 3.10 A; A/B/C/D/E/F/G/H/I/J=22-343.
DR   PDB; 6CDU; X-ray; 3.45 A; A/B/C/D/E/F/G/H/I/J=22-220.
DR   PDB; 6D1S; X-ray; 3.20 A; A/B/C/D/E/F/G/H/I/J=22-220.
DR   PDB; 6V03; EM; 3.30 A; A/B/C/D/E=22-341.
DR   PDB; 6V0B; EM; 4.10 A; A/B/C/D/E=22-343.
DR   PDB; 7L6Q; EM; 2.50 A; A/B/C/D/E=22-343.
DR   PDB; 7L6U; EM; 3.30 A; A/B/C/D/E=22-343.
DR   PDB; 8D63; EM; 3.14 A; A/B/C/D/E=22-343.
DR   PDB; 8D64; EM; 3.14 A; A/B/C/D/E=32-338.
DR   PDB; 8D65; EM; 3.47 A; A/B/C/D/E=22-343.
DR   PDB; 8D66; EM; 3.14 A; A/B/C/D/E=22-343.
DR   PDB; 8D67; EM; 3.30 A; A/B/C/D/E=22-343.
DR   PDB; 8D68; EM; 3.36 A; A/B/C/D/E=22-343.
DR   PDB; 8F32; EM; 3.71 A; A/B/C/D/E=22-343.
DR   PDB; 8F33; EM; 3.28 A; A/B/C/D/E=22-343.
DR   PDB; 8F34; EM; 3.12 A; A/B/C/D/E=22-343.
DR   PDB; 8F35; EM; 3.17 A; A/B/C/D/E=22-343.
DR   PDBsum; 2YN6; -.
DR   PDBsum; 3RQU; -.
DR   PDBsum; 3RQW; -.
DR   PDBsum; 3UQ4; -.
DR   PDBsum; 3UQ5; -.
DR   PDBsum; 3UQ7; -.
DR   PDBsum; 4Z90; -.
DR   PDBsum; 4Z91; -.
DR   PDBsum; 5HEJ; -.
DR   PDBsum; 5HEO; -.
DR   PDBsum; 5HEU; -.
DR   PDBsum; 5HEW; -.
DR   PDBsum; 5LG3; -.
DR   PDBsum; 5SXU; -.
DR   PDBsum; 6CDU; -.
DR   PDBsum; 6D1S; -.
DR   PDBsum; 6V03; -.
DR   PDBsum; 6V0B; -.
DR   AlphaFoldDB; E0SJQ4; -.
DR   EMDB; EMD-20968; -.
DR   EMDB; EMD-20986; -.
DR   EMDB; EMD-23207; -.
DR   EMDB; EMD-23208; -.
DR   EMDB; EMD-28829; -.
DR   EMDB; EMD-28830; -.
DR   EMDB; EMD-28831; -.
DR   EMDB; EMD-28832; -.
DR   SMR; E0SJQ4; -.
DR   DIP; DIP-60036N; -.
DR   STRING; 198628.Dda3937_00520; -.
DR   GeneID; 9735629; -.
DR   KEGG; ddd:Dda3937_00520; -.
DR   PATRIC; fig|198628.6.peg.4084; -.
DR   eggNOG; COG5361; Bacteria.
DR   HOGENOM; CLU_010920_3_0_6; -.
DR   OrthoDB; 1326189at2; -.
DR   Proteomes; UP000006859; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005230; F:extracellular ligand-gated monoatomic ion channel activity; IEA:InterPro.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR   CDD; cd18988; LGIC_ECD_bact; 1.
DR   CDD; cd03559; LGIC_TM; 1.
DR   Gene3D; 2.70.170.10; Neurotransmitter-gated ion-channel ligand-binding domain; 1.
DR   Gene3D; 1.20.58.390; Neurotransmitter-gated ion-channel transmembrane domain; 1.
DR   InterPro; IPR006202; Neur_chan_lig-bd.
DR   InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR   InterPro; IPR006201; Neur_channel.
DR   InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR   InterPro; IPR038050; Neuro_actylchol_rec.
DR   PANTHER; PTHR18945; NEUROTRANSMITTER GATED ION CHANNEL; 1.
DR   PANTHER; PTHR18945:SF925; PROTON-GATED ION CHANNEL; 1.
DR   Pfam; PF02931; Neur_chan_LBD; 1.
DR   SUPFAM; SSF90112; Neurotransmitter-gated ion-channel transmembrane pore; 1.
DR   SUPFAM; SSF63712; Nicotinic receptor ligand binding domain-like; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:2YN6, ECO:0007829|PDB:3RQU};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Receptor {ECO:0000313|EMBL:ADN00343.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006859};
KW   Signal {ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..343
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5003140389"
FT   TRANSMEM        224..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        257..274
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        286..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        323..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          28..221
FT                   /note="Neurotransmitter-gated ion-channel ligand-binding"
FT                   /evidence="ECO:0000259|Pfam:PF02931"
SQ   SEQUENCE   343 AA;  39175 MW;  90663D9956C5C82E CRC64;
     MRILWSCCGL VLLLLGLPAW SAPADNAADA RPVDVSVSIF INKIYGVNTL EQTYKVDGYI
     VAQWTGKPRK TPGDKPLIVE NTQIERWINN GLWVPALEFI NVVGSPDTGN KRLMLFPDGR
     VIYNARFLGS FSNDMDFRLF PFDRQQFVLE LEPFSYNNQQ LRFSDIQVYT ENIDNEEIDE
     WWIRGKASTH ISDIRYDHLS SVQPNQNEFS RITVRIDAVR NPSYYLWSFI LPLGLIIAAS
     WSVFWLESFS ERLQTSFTLM LTVVAYAFYT SNILPRLPYT TVIDQMIIAG YGSIFAAILL
     IIFAHHRQAN GVEDDLLIQR CRLAFPLGFL AIGCVLVIRG ITL
//