Skip Header

Contribute Send feedback
Read comments (?) or add your own

E0RYM8 (E0RYM8_BUTPB) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Probable dual-specificity RNA methyltransferase RlmN HAMAP-Rule MF_01849
EC=2.1.1.- HAMAP-Rule MF_01849
EC=2.1.1.192 HAMAP-Rule MF_01849
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase HAMAP-Rule MF_01849
23S rRNA m2A2503 methyltransferase HAMAP-Rule MF_01849
Ribosomal RNA large subunit methyltransferase N HAMAP-Rule MF_01849
tRNA (adenine(37)-C(2))-methyltransferase HAMAP-Rule MF_01849
tRNA m2A37 methyltransferase HAMAP-Rule MF_01849
Gene names
Name:rlmN HAMAP-Rule MF_01849
Ordered Locus Names:bpr_I1989 EMBL ADL34723.1
OrganismButyrivibrio proteoclasticus (strain ATCC 51982 / DSM 14932 / B316) (Clostridium proteoclasticum) [Complete proteome] [HAMAP]
Taxonomic identifier515622 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae › Butyrivibrio ›

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs By similarity. HAMAP-Rule MF_01849

Catalytic activity

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA. HAMAP-Rule MF_01849 SAAS SAAS004383

2 S-adenosyl-L-methionine + adenine37 in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA. HAMAP-Rule MF_01849 SAAS SAAS004383

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_01849 SAAS SAAS004383

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01849.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue By similarity. HAMAP-Rule MF_01849

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family. HAMAP-Rule MF_01849

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region167 – 1682S-adenosyl-L-methionine binding By similarity HAMAP-Rule MF_01849
Region222 – 2243S-adenosyl-L-methionine binding By similarity HAMAP-Rule MF_01849

Sites

Active site1001Proton acceptor By similarity HAMAP-Rule MF_01849
Active site3411S-methylcysteine intermediate By similarity HAMAP-Rule MF_01849
Metal binding1201Iron-sulfur (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01849
Metal binding1241Iron-sulfur (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01849
Metal binding1271Iron-sulfur (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_01849
Binding site1991S-adenosyl-L-methionine By similarity HAMAP-Rule MF_01849
Binding site2981S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_01849

Amino acid modifications

Disulfide bond113 ↔ 341(transient) By similarity HAMAP-Rule MF_01849

Sequences

Sequence LengthMass (Da)Tools
E0RYM8 [UniParc].

Last modified November 2, 2010. Version 1.
Checksum: D86E367A65697023

FASTA35239,830
        10         20         30         40         50         60 
MSYIQGINGK IDIKSLTLDE LTILIKEAGE PAFRAKQLYE WMHVKLARDY DEMTNIPKSL 

        70         80         90        100        110        120 
KEKCREMFDF VSLKSELVQE SKLDDTKKFL FALSDGNMIE SVFMKYKFGV SVCISSQVGC 

       130        140        150        160        170        180 
RMGCKFCAST IDGVVRNLLP SEMLDQIYAI SRITGEKVGR VVVMGSGEPL DNYDNLLRFI 

       190        200        210        220        230        240 
DLLTNEDGLN MSQRNLTVST CGIVPNILRL ADKNLAINLA LSLHASNNDK RKELMPIANK 

       250        260        270        280        290        300 
YEIHEVLDAC RTYFDKTGRQ LTFEYSLVAG VNDTDEDARE LTDLLSGFNC VVNLIPVNPI 

       310        320        330        340        350 
KERDFKPTDR AGALGFKNKL EKSRINVTIR REMGRDIDGA CGQLRRRHLE EN

« Hide

References

[1]"The glycobiome of the rumen bacterium Butyrivibrio proteoclasticus B316(T) highlights adaptation to a polysaccharide-rich environment."
Kelly W.J., Leahy S.C., Altermann E., Yeoman C.J., Dunne J.C., Kong Z., Pacheco D.M., Li D., Noel S.J., Moon C.D., Cookson A.L., Attwood G.T.
PLoS ONE 5:E11942-E11942(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51982 / DSM 14932 / B316.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001810 Genomic DNA. Translation: ADL34723.1.
RefSeqYP_003831305.1. NC_014387.1.

3D structure databases

ProteinModelPortalE0RYM8.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL34723; ADL34723; bpr_I1989.
GeneID9575636.
KEGGbpb:bpr_I1989.
PATRIC42223285. VBIButPro41874_2008.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000217991.
KOK06941.
OMAIYHFGVS.

Enzyme and pathway databases

BioCycBPRO515622:GHKV-2854-MONOMER.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01849. 23SrRNA_methyltr_N.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR004383. rRNA_lsu_MTrfase_RlmN.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF006004. CHP00048. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00048. TIGR00048. 1 hit.
ProtoNetSearch...

Entry information

Entry nameE0RYM8_BUTPB
AccessionPrimary (citable) accession number: E0RYM8
Entry history
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: May 1, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info