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E0RTD9

- PFKA_SPITD

UniProt

E0RTD9 - PFKA_SPITD

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 161ATP; via amide nitrogenUniRule annotation
Metal bindingi119 – 1191Magnesium; catalyticUniRule annotation
Sitei120 – 1201Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei143 – 1431Proton acceptorUniRule annotation
Binding sitei178 – 1781Substrate; shared with dimeric partnerUniRule annotation
Binding sitei238 – 2381SubstrateUniRule annotation
Binding sitei282 – 2821Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 792ATPUniRule annotation
Nucleotide bindingi118 – 1214ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE665571:GI3Y-29-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Synonyms:pfkA3
Ordered Locus Names:STHERM_c00290
OrganismiSpirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)
Taxonomic identifieri665571 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeSpirochaeta
ProteomesiUP000001296: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366ATP-dependent 6-phosphofructokinasePRO_0000429709Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 1433Substrate bindingUniRule annotation
Regioni185 – 1873Substrate bindingUniRule annotation
Regioni288 – 2914Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248869.
KOiK00850.
OMAiASECIDR.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

E0RTD9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRPATRTFG ILTSGGDCPG LNAAIRGVTK AAYWRYGMSI IGISHGYRGL
60 70 80 90 100
IEGDARLLHP RDFDGILTRG GTILGTSREK PFKPDPGEKD SEAGSRKVEA
110 120 130 140 150
IIENYHKLHL DCLVVLGGNG THKTAYLLQQ AGLNVIGLPK TIDNDIWGTD
160 170 180 190 200
VTFGFHSAVD IATEAIDRLH STAHAHNRVM VIEVMGHKAG WLALYAGIAG
210 220 230 240 250
GGDIILIPEI PYDLAHIVHH LQTRQQRGKE FSIVVVAEGA LSREESLMSK
260 270 280 290 300
EERKKRRKKN RFPTKGYEVA HLIQEATGME TRVTVLGYLQ RGGTPSPYDR
310 320 330 340 350
LLATRFGTAA AELLYRGDYG KMVALRDGEV VAIPLGEVAE KLKTVPPDHP
360
LIDTARAVGT CFGDGV
Length:366
Mass (Da):39,845
Last modified:November 2, 2010 - v1
Checksum:i7D2B4DE9F78372AB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001698 Genomic DNA. Translation: ADN01005.1.
AF342985 Genomic DNA. Translation: AAK16737.1.
RefSeqiWP_013312846.1. NC_014484.1.
YP_003873278.1. NC_014484.1.

Genome annotation databases

EnsemblBacteriaiADN01005; ADN01005; STHERM_c00290.
GeneIDi9755283.
KEGGista:STHERM_c00290.
PATRICi42519253. VBISpiThe146732_0030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001698 Genomic DNA. Translation: ADN01005.1 .
AF342985 Genomic DNA. Translation: AAK16737.1 .
RefSeqi WP_013312846.1. NC_014484.1.
YP_003873278.1. NC_014484.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADN01005 ; ADN01005 ; STHERM_c00290 .
GeneIDi 9755283.
KEGGi sta:STHERM_c00290.
PATRICi 42519253. VBISpiThe146732_0030.

Phylogenomic databases

HOGENOMi HOG000248869.
KOi K00850.
OMAi ASECIDR.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci STHE665571:GI3Y-29-MONOMER.

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the polysaccharide-degrading, thermophilic anaerobe Spirochaeta thermophila DSM 6192."
    Angelov A., Liebl S., Ballschmiter M., Bomeke M., Lehmann R., Liesegang H., Daniel R., Liebl W.
    J. Bacteriol. 192:6492-6493(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49972 / DSM 6192 / RI 19.B1.
  2. "Sequencing, high-level expression and phylogeny of the pyrophosphate-dependent phosphofructokinase from the thermophilic spirochete Spirochaeta thermophila."
    Ronimus R., de Heus E., Ruckert A., Morgan H.
    Arch. Microbiol. 175:308-312(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-145.

Entry informationi

Entry nameiPFKA_SPITD
AccessioniPrimary (citable) accession number: E0RTD9
Secondary accession number(s): Q9AG65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: November 2, 2010
Last modified: October 29, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3