Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E0RTD9 (PFKA_SPITD) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfkA3
Ordered Locus Names:STHERM_c00290
OrganismSpirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1) [Complete proteome] [HAMAP]
Taxonomic identifier665571 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeSpirochaeta

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_01976
PRO_0000429709

Regions

Nucleotide binding78 – 792ATP By similarity
Nucleotide binding118 – 1214ATP By similarity
Region141 – 1433Substrate binding By similarity
Region185 – 1873Substrate binding By similarity
Region288 – 2914Substrate binding By similarity

Sites

Active site1431Proton acceptor By similarity
Metal binding1191Magnesium; catalytic By similarity
Binding site161ATP; via amide nitrogen By similarity
Binding site1781Substrate; shared with dimeric partner By similarity
Binding site2381Substrate By similarity
Binding site2821Substrate; shared with dimeric partner By similarity
Site1201Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
E0RTD9 [UniParc].

Last modified November 2, 2010. Version 1.
Checksum: 7D2B4DE9F78372AB

FASTA36639,845
        10         20         30         40         50         60 
MKRPATRTFG ILTSGGDCPG LNAAIRGVTK AAYWRYGMSI IGISHGYRGL IEGDARLLHP 

        70         80         90        100        110        120 
RDFDGILTRG GTILGTSREK PFKPDPGEKD SEAGSRKVEA IIENYHKLHL DCLVVLGGNG 

       130        140        150        160        170        180 
THKTAYLLQQ AGLNVIGLPK TIDNDIWGTD VTFGFHSAVD IATEAIDRLH STAHAHNRVM 

       190        200        210        220        230        240 
VIEVMGHKAG WLALYAGIAG GGDIILIPEI PYDLAHIVHH LQTRQQRGKE FSIVVVAEGA 

       250        260        270        280        290        300 
LSREESLMSK EERKKRRKKN RFPTKGYEVA HLIQEATGME TRVTVLGYLQ RGGTPSPYDR 

       310        320        330        340        350        360 
LLATRFGTAA AELLYRGDYG KMVALRDGEV VAIPLGEVAE KLKTVPPDHP LIDTARAVGT 


CFGDGV 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the polysaccharide-degrading, thermophilic anaerobe Spirochaeta thermophila DSM 6192."
Angelov A., Liebl S., Ballschmiter M., Bomeke M., Lehmann R., Liesegang H., Daniel R., Liebl W.
J. Bacteriol. 192:6492-6493(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49972 / DSM 6192 / RI 19.B1.
[2]"Sequencing, high-level expression and phylogeny of the pyrophosphate-dependent phosphofructokinase from the thermophilic spirochete Spirochaeta thermophila."
Ronimus R., de Heus E., Ruckert A., Morgan H.
Arch. Microbiol. 175:308-312(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-145.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001698 Genomic DNA. Translation: ADN01005.1.
AF342985 Genomic DNA. Translation: AAK16737.1.
RefSeqYP_003873278.1. NC_014484.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADN01005; ADN01005; STHERM_c00290.
GeneID9755283.
KEGGsta:STHERM_c00290.
PATRIC42519253. VBISpiThe146732_0030.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000248869.
KOK00850.
OMAASECIDR.

Enzyme and pathway databases

BioCycSTHE665571:GI3Y-29-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA_SPITD
AccessionPrimary (citable) accession number: E0RTD9
Secondary accession number(s): Q9AG65
Entry history
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: November 2, 2010
Last modified: July 9, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways