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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase (pfp), ATP-dependent 6-phosphofructokinase (pfkA), ATP-dependent 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 161ATP; via amide nitrogenUniRule annotation
Metal bindingi119 – 1191Magnesium; catalyticUniRule annotation
Sitei120 – 1201Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei143 – 1431Proton acceptorUniRule annotation
Binding sitei178 – 1781Substrate; shared with dimeric partnerUniRule annotation
Binding sitei238 – 2381SubstrateUniRule annotation
Binding sitei282 – 2821Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 792ATPUniRule annotation
Nucleotide bindingi118 – 1214ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE665571:GI3Y-29-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Synonyms:pfkA3
Ordered Locus Names:STHERM_c00290
OrganismiSpirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)
Taxonomic identifieri665571 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeSpirochaeta
ProteomesiUP000001296 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366ATP-dependent 6-phosphofructokinasePRO_0000429709Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi665571.STHERM_c00290.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 1433Substrate bindingUniRule annotation
Regioni185 – 1873Substrate bindingUniRule annotation
Regioni288 – 2914Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248869.
KOiK00850.
OMAiESHHRVL.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

E0RTD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRPATRTFG ILTSGGDCPG LNAAIRGVTK AAYWRYGMSI IGISHGYRGL
60 70 80 90 100
IEGDARLLHP RDFDGILTRG GTILGTSREK PFKPDPGEKD SEAGSRKVEA
110 120 130 140 150
IIENYHKLHL DCLVVLGGNG THKTAYLLQQ AGLNVIGLPK TIDNDIWGTD
160 170 180 190 200
VTFGFHSAVD IATEAIDRLH STAHAHNRVM VIEVMGHKAG WLALYAGIAG
210 220 230 240 250
GGDIILIPEI PYDLAHIVHH LQTRQQRGKE FSIVVVAEGA LSREESLMSK
260 270 280 290 300
EERKKRRKKN RFPTKGYEVA HLIQEATGME TRVTVLGYLQ RGGTPSPYDR
310 320 330 340 350
LLATRFGTAA AELLYRGDYG KMVALRDGEV VAIPLGEVAE KLKTVPPDHP
360
LIDTARAVGT CFGDGV
Length:366
Mass (Da):39,845
Last modified:November 2, 2010 - v1
Checksum:i7D2B4DE9F78372AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001698 Genomic DNA. Translation: ADN01005.1.
AF342985 Genomic DNA. Translation: AAK16737.1.
RefSeqiWP_013312846.1. NC_014484.1.
YP_003873278.1. NC_014484.1.

Genome annotation databases

EnsemblBacteriaiADN01005; ADN01005; STHERM_c00290.
KEGGista:STHERM_c00290.
PATRICi42519253. VBISpiThe146732_0030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001698 Genomic DNA. Translation: ADN01005.1.
AF342985 Genomic DNA. Translation: AAK16737.1.
RefSeqiWP_013312846.1. NC_014484.1.
YP_003873278.1. NC_014484.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi665571.STHERM_c00290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADN01005; ADN01005; STHERM_c00290.
KEGGista:STHERM_c00290.
PATRICi42519253. VBISpiThe146732_0030.

Phylogenomic databases

HOGENOMiHOG000248869.
KOiK00850.
OMAiESHHRVL.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciSTHE665571:GI3Y-29-MONOMER.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR000023. Phosphofructokinase_dom.
IPR012829. Phosphofructokinase_III.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the polysaccharide-degrading, thermophilic anaerobe Spirochaeta thermophila DSM 6192."
    Angelov A., Liebl S., Ballschmiter M., Bomeke M., Lehmann R., Liesegang H., Daniel R., Liebl W.
    J. Bacteriol. 192:6492-6493(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49972 / DSM 6192 / RI 19.B1.
  2. "Sequencing, high-level expression and phylogeny of the pyrophosphate-dependent phosphofructokinase from the thermophilic spirochete Spirochaeta thermophila."
    Ronimus R., de Heus E., Ruckert A., Morgan H.
    Arch. Microbiol. 175:308-312(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-145.

Entry informationi

Entry nameiPFKA_SPITD
AccessioniPrimary (citable) accession number: E0RTD9
Secondary accession number(s): Q9AG65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: November 2, 2010
Last modified: June 24, 2015
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.