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E0RTD9

- PFKA_SPITD

UniProt

E0RTD9 - PFKA_SPITD

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei16 – 161ATP; via amide nitrogenUniRule annotation
    Metal bindingi119 – 1191Magnesium; catalyticUniRule annotation
    Sitei120 – 1201Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
    Active sitei143 – 1431Proton acceptorUniRule annotation
    Binding sitei178 – 1781Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei238 – 2381SubstrateUniRule annotation
    Binding sitei282 – 2821Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 792ATPUniRule annotation
    Nucleotide bindingi118 – 1214ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSTHE665571:GI3Y-29-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Synonyms:pfkA3
    Ordered Locus Names:STHERM_c00290
    OrganismiSpirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)
    Taxonomic identifieri665571 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeSpirochaeta
    ProteomesiUP000001296: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366ATP-dependent 6-phosphofructokinasePRO_0000429709Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni141 – 1433Substrate bindingUniRule annotation
    Regioni185 – 1873Substrate bindingUniRule annotation
    Regioni288 – 2914Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiASECIDR.

    Family and domain databases

    HAMAPiMF_01976. Phosphofructokinase_III.
    InterProiIPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    E0RTD9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRPATRTFG ILTSGGDCPG LNAAIRGVTK AAYWRYGMSI IGISHGYRGL    50
    IEGDARLLHP RDFDGILTRG GTILGTSREK PFKPDPGEKD SEAGSRKVEA 100
    IIENYHKLHL DCLVVLGGNG THKTAYLLQQ AGLNVIGLPK TIDNDIWGTD 150
    VTFGFHSAVD IATEAIDRLH STAHAHNRVM VIEVMGHKAG WLALYAGIAG 200
    GGDIILIPEI PYDLAHIVHH LQTRQQRGKE FSIVVVAEGA LSREESLMSK 250
    EERKKRRKKN RFPTKGYEVA HLIQEATGME TRVTVLGYLQ RGGTPSPYDR 300
    LLATRFGTAA AELLYRGDYG KMVALRDGEV VAIPLGEVAE KLKTVPPDHP 350
    LIDTARAVGT CFGDGV 366
    Length:366
    Mass (Da):39,845
    Last modified:November 2, 2010 - v1
    Checksum:i7D2B4DE9F78372AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001698 Genomic DNA. Translation: ADN01005.1.
    AF342985 Genomic DNA. Translation: AAK16737.1.
    RefSeqiYP_003873278.1. NC_014484.1.

    Genome annotation databases

    EnsemblBacteriaiADN01005; ADN01005; STHERM_c00290.
    GeneIDi9755283.
    KEGGista:STHERM_c00290.
    PATRICi42519253. VBISpiThe146732_0030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001698 Genomic DNA. Translation: ADN01005.1 .
    AF342985 Genomic DNA. Translation: AAK16737.1 .
    RefSeqi YP_003873278.1. NC_014484.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADN01005 ; ADN01005 ; STHERM_c00290 .
    GeneIDi 9755283.
    KEGGi sta:STHERM_c00290.
    PATRICi 42519253. VBISpiThe146732_0030.

    Phylogenomic databases

    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi ASECIDR.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci STHE665571:GI3Y-29-MONOMER.

    Family and domain databases

    HAMAPi MF_01976. Phosphofructokinase_III.
    InterProi IPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the polysaccharide-degrading, thermophilic anaerobe Spirochaeta thermophila DSM 6192."
      Angelov A., Liebl S., Ballschmiter M., Bomeke M., Lehmann R., Liesegang H., Daniel R., Liebl W.
      J. Bacteriol. 192:6492-6493(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49972 / DSM 6192 / RI 19.B1.
    2. "Sequencing, high-level expression and phylogeny of the pyrophosphate-dependent phosphofructokinase from the thermophilic spirochete Spirochaeta thermophila."
      Ronimus R., de Heus E., Ruckert A., Morgan H.
      Arch. Microbiol. 175:308-312(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 45-145.

    Entry informationi

    Entry nameiPFKA_SPITD
    AccessioniPrimary (citable) accession number: E0RTD9
    Secondary accession number(s): Q9AG65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 9, 2014
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3