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Protein

D-alanine--D-alanine ligase

Gene

STHERM_c06000

Organism
Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Cell wall formation.SAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.SAAS annotation

Cofactori

Mg2+SAAS annotation, Mn2+SAAS annotationNote: Binds 2 magnesium or manganese ions per subunit.SAAS annotation

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. D-alanine-D-alanine ligase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  3. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseSAAS annotationImported

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradationSAAS annotation, Peptidoglycan synthesisSAAS annotation

Keywords - Ligandi

ATP-bindingSAAS annotation, MagnesiumSAAS annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE665571:GI3Y-600-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseSAAS annotation (EC:6.3.2.4SAAS annotation)
Gene namesi
Ordered Locus Names:STHERM_c06000Imported
OrganismiSpirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)Imported
Taxonomic identifieri665571 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeSpirochaeta
ProteomesiUP000001296 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm SAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliE0RQQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains ATP-grasp domain.SAAS annotation

Phylogenomic databases

HOGENOMiHOG000244781.
KOiK01921.
OMAiNDPRKEL.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.150. 2 hits.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR025714. Methyltranfer_dom.
IPR016185. PreATP-grasp_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

E0RQQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKVRDTDK PPERRRRRIP AARARCLGPL SDLESHVAPD WWSRIFNSYY
60 70 80 90 100
MKTDGDVVDD LAITRREVDL FTSVLDLRED EAVLDLCCGQ GRHSLELARR
110 120 130 140 150
GFTKVEGLDR SHYLIQRAKA QAAKEGIPVR FREGDARKLP HPPDQFDVVM
160 170 180 190 200
ILGNSFGYFE TSKDDLLVLK EVARVLKPWG RVLLDLADGD FLRAHYVPRS
210 220 230 240 250
WEWIDPKHFV CRERALSADR QRLISREVIV HTEKGVEVDQ FYAERLYTPE
260 270 280 290 300
LIRSLLEEAG FQEVSFHGTL TPDSARGQDL GMMERRILVS ARIRKEWTPV
310 320 330 340 350
KPKKKNVSTQ VAVVMGDPSK PDPLKPFMVF DDDDFYTIDR LKAALKECTG
360 370 380 390 400
YSFTYLSRHE TLIQDLMRLK GKIDYVFNLC DEGFFNDPRK ELHVPALLEM
410 420 430 440 450
LGIPYTGATP QGLAFCYDKS LVRGVAKEMG VPVPRAFFID PTSIVFEVPF
460 470 480 490 500
GFPMIVKPNL GDSSFGITQK SVVWSLDELS ACISEIRDTF GYEKPVLVEE
510 520 530 540 550
FLTGKDLTVG IIGNPPDDYM VLPIVEEDYS ALPPELPRIC GYEAKWLPDS
560 570 580 590 600
PYWKALQSIP AGLPEATEKS IVEWCVRLFE RLECRDYARF DWRCDADGNP
610 620 630 640
YLLEVNPNPG WCWDGHLAKM ARLAGMEYPQ MLQAILKAAE RRLGIR
Length:646
Mass (Da):73,893
Last modified:November 2, 2010 - v1
Checksum:iAD1D2FC207D98DDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001698 Genomic DNA. Translation: ADN01559.1.
RefSeqiYP_003873832.1. NC_014484.1.

Genome annotation databases

EnsemblBacteriaiADN01559; ADN01559; STHERM_c06000.
KEGGista:STHERM_c06000.
PATRICi42520415. VBISpiThe146732_0594.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001698 Genomic DNA. Translation: ADN01559.1.
RefSeqiYP_003873832.1. NC_014484.1.

3D structure databases

ProteinModelPortaliE0RQQ5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADN01559; ADN01559; STHERM_c06000.
KEGGista:STHERM_c06000.
PATRICi42520415. VBISpiThe146732_0594.

Phylogenomic databases

HOGENOMiHOG000244781.
KOiK01921.
OMAiNDPRKEL.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciSTHE665571:GI3Y-600-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.150. 2 hits.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR025714. Methyltranfer_dom.
IPR016185. PreATP-grasp_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Spirochaeta thermophila DSM6192."
    Angelov A., Mientus M., Wittenberg S., Lehmann R., Liesegang H., Daniel R., Liebl W.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 6192.
  2. "Genome sequence of the polysaccharide-degrading, thermophilic anaerobe Spirochaeta thermophila DSM 6192."
    Angelov A., Liebl S., Ballschmiter M., Bomeke M., Lehmann R., Liesegang H., Daniel R., Liebl W.
    J. Bacteriol. 192:6492-6493(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49972 / DSM 6192 / RI 19.B1Imported.

Entry informationi

Entry nameiE0RQQ5_SPITD
AccessioniPrimary (citable) accession number: E0RQQ5
Entry historyi
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: April 29, 2015
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.