ID   E0RKY5_PAEP6            Unreviewed;       497 AA.
AC   E0RKY5;
DT   02-NOV-2010, integrated into UniProtKB/TrEMBL.
DT   02-NOV-2010, sequence version 1.
DT   27-MAR-2024, entry version 62.
DE   SubName: Full=Alpha-amylase {ECO:0000313|EMBL:ADM72422.1};
GN   OrderedLocusNames=PPE_04663 {ECO:0000313|EMBL:ADM72422.1};
OS   Paenibacillus polymyxa (strain E681).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=349520 {ECO:0000313|EMBL:ADM72422.1, ECO:0000313|Proteomes:UP000002227};
RN   [1] {ECO:0000313|EMBL:ADM72422.1, ECO:0000313|Proteomes:UP000002227}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E681 {ECO:0000313|EMBL:ADM72422.1,
RC   ECO:0000313|Proteomes:UP000002227};
RX   PubMed=20851896; DOI=10.1128/JB.00983-10;
RA   Kim J.F., Jeong H., Park S.Y., Kim S.B., Park Y.K., Choi S.K., Ryu C.M.,
RA   Hur C.G., Ghim S.Y., Oh T.K., Kim J.J., Park C.S., Park S.H.;
RT   "Genome sequence of the polymyxin-producing plant-probiotic rhizobacterium
RT   Paenibacillus polymyxa E681.";
RL   J. Bacteriol. 192:6103-6104(2010).
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913};
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DR   EMBL; CP000154; ADM72422.1; -; Genomic_DNA.
DR   RefSeq; WP_013312530.1; NZ_CP048794.1.
DR   AlphaFoldDB; E0RKY5; -.
DR   CAZy; GH13; Glycoside Hydrolase Family 13.
DR   KEGG; ppy:PPE_04663; -.
DR   eggNOG; COG0366; Bacteria.
DR   HOGENOM; CLU_024572_2_0_9; -.
DR   Proteomes; UP000002227; Chromosome.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0033927; F:glucan 1,4-alpha-maltohexaosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR   Gene3D; 2.40.30.140; -; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR013776; A-amylase_thermo.
DR   InterPro; IPR015237; Alpha-amylase_C_pro.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR   Pfam; PF09154; Alpha-amy_C_pro; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   4: Predicted;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2}.
FT   DOMAIN          5..391
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   ACT_SITE        233
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   ACT_SITE        263
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT   BINDING         104
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         196
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         204
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT   BINDING         237
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ   SEQUENCE   497 AA;  57120 MW;  21546F3AC1E6F560 CRC64;
     MKRNHTMMQF FEWNVEADGS HWKKLARLAP ELKAKGIDAI WIPPVTKGQS PEDTGYGVYD
     LYDLGEFDQK GAVRTKYGTR EDLLEAVAAC VRHGVAVYVD LVMNHKAGAD ETEVFKVVEV
     NPDNRNEVIS EPFDIEGWTK FTFPGRQGKY STFQWNFEHF NGTDYDASQD RTGIYRILGK
     NKSWSENVDD EFGNYDYLMF ANIDYNHQDV RKEMIRWGKW LVDTLQCNGF RLDAIKHINH
     EFVREFATEM IKKRGQDFYM VGEFWKPDLE SCQKFLDTID YKIDLFDVSL HYKLHSASLG
     GKDFDLSTIF EDTLVHTHPL NSVTFVDNHD SQPHEALESW VEDWFKPSAY ALILLRKDGY
     PCVFYGDYYG IQGQTPVKGK QAELDPLLYA RYHKAYGEQK DYFDDPHTIG WVRQGVPELE
     GSGCAVVITN ANDGEKRMFV GKQHAAGEWI DLTGHHDHTV KIEQDGYGIF PVRAGSVSVW
     ALPAEEDSQE TEEEANA
//