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E0REK9

- E0REK9_PAEP6

UniProt

E0REK9 - E0REK9_PAEP6

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Paenibacillus polymyxa (strain E681)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
    Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
    Active sitei130 – 1301Proton acceptorUniRule annotation
    Binding sitei157 – 1571Allosteric activator ADPUniRule annotation
    Binding sitei165 – 1651Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei225 – 2251SubstrateUniRule annotation
    Binding sitei247 – 2471Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 762ATPUniRule annotation
    Nucleotide bindingi105 – 1084ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPPOL349520:GH6J-1694-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:PPE_01700Imported
    OrganismiPaenibacillus polymyxa (strain E681)Imported
    Taxonomic identifieri349520 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus
    ProteomesiUP000002227: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni128 – 1303Substrate bindingUniRule annotation
    Regioni172 – 1743Substrate bindingUniRule annotation
    Regioni188 – 1903Allosteric activator ADP bindingUniRule annotation
    Regioni216 – 2183Allosteric activator ADP bindingUniRule annotation
    Regioni253 – 2564Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiYRRGKLH.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    E0REK9-1 [UniParc]FASTAAdd to Basket

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    MTAVKKIAVL TSGGDSQGMN AAVRAVVRSG LYFGLEVFGI QRGYQGLLND    50
    DIFPMDLRSV GDIIQRGGTV LQSARCLEFT TEEGQKKGAQ ILRDRGIDGV 100
    VVIGGDGSYQ GANKLTKQGI KTMCLPGTID NDISFTDYTI GFDTAVSIVV 150
    DAINKLRDTM SSHERSSIVE VMGRHCGDIA LHAGLASGAE TILVPEVEFD 200
    LNEVSDRMKQ NFKHGKRHSI IIVAEGVGKG ENVAQVIQEN CPDYEPRVTV 250
    LGHIQRGGTP TAFDRNLASR LGDFAVRQLI EGVSDKACGT IDGKLVATDI 300
    DKVVNTKKDF NMELYELALR LSQ 323
    Length:323
    Mass (Da):34,892
    Last modified:November 2, 2010 - v1
    Checksum:iC9F3B9567613A0BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000154 Genomic DNA. Translation: ADM69537.1.
    RefSeqiWP_013309709.1. NC_014483.1.
    YP_003870075.1. NC_014483.1.

    Genome annotation databases

    EnsemblBacteriaiADM69537; ADM69537; PPE_01700.
    GeneIDi9774453.
    KEGGippy:PPE_01700.
    PATRICi42409090. VBIPaePol94484_1686.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000154 Genomic DNA. Translation: ADM69537.1 .
    RefSeqi WP_013309709.1. NC_014483.1.
    YP_003870075.1. NC_014483.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADM69537 ; ADM69537 ; PPE_01700 .
    GeneIDi 9774453.
    KEGGi ppy:PPE_01700.
    PATRICi 42409090. VBIPaePol94484_1686.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi YRRGKLH.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci PPOL349520:GH6J-1694-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the polymyxin-producing plant-probiotic rhizobacterium Paenibacillus polymyxa E681."
      Kim J.F., Jeong H., Park S.Y., Kim S.B., Park Y.K., Choi S.K., Ryu C.M., Hur C.G., Ghim S.Y., Oh T.K., Kim J.J., Park C.S., Park S.H.
      J. Bacteriol. 192:6103-6104(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: E681Imported.

    Entry informationi

    Entry nameiE0REK9_PAEP6
    AccessioniPrimary (citable) accession number: E0REK9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: November 2, 2010
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteomeImported

    External Data

    Dasty 3