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E0J788 (E0J788_ECOLW) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
6-phosphofructokinase HAMAP-Rule MF_00339
Short name=Phosphofructokinase HAMAP-Rule MF_00339
EC=2.7.1.11 HAMAP-Rule MF_00339
Alternative name(s):
Phosphohexokinase HAMAP-Rule MF_00339
Gene names
Name:pfkA HAMAP-Rule MF_00339 EMBL ADT77574.1
Ordered Locus Names:ECW_m4268 EMBL ADT77574.1, WFL_20810 EMBL AFH13804.1
ORF Names:EschWDRAFT_4446 EMBL EFN35972.1
OrganismEscherichia coli (strain ATCC 9637 / CCM 2024 / DSM 1116 / NCIMB 8666 / NRRL B-766 / W) [Complete proteome] [HAMAP] EMBL EFN35972.1
Taxonomic identifier566546 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339 SAAS SAAS000023

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339 SAAS SAAS000023

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339 SAAS SAAS000023.

Sequence similarities

Belongs to the phosphofructokinase family. HAMAP-Rule MF_00339

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding22 – 265ATP By similarity HAMAP-Rule MF_00339
Nucleotide binding155 – 1595ATP By similarity HAMAP-Rule MF_00339
Nucleotide binding172 – 18817ATP By similarity HAMAP-Rule MF_00339

Sites

Active site1281Proton acceptor By similarity HAMAP-Rule MF_00339
Metal binding1861Magnesium; via carbonyl oxygen By similarity HAMAP-Rule MF_00339
Metal binding1881Magnesium By similarity HAMAP-Rule MF_00339
Binding site1631Substrate By similarity HAMAP-Rule MF_00339
Binding site2441Substrate By similarity HAMAP-Rule MF_00339
Binding site2501Substrate By similarity HAMAP-Rule MF_00339
Binding site2531Substrate By similarity HAMAP-Rule MF_00339

Sequences

Sequence LengthMass (Da)Tools
E0J788 [UniParc].

Last modified November 2, 2010. Version 1.
Checksum: D03D79F6A5536A41

FASTA32034,842
        10         20         30         40         50         60 
MIKKIGVLTS GGDAPGMNAA IRGVVRSALT EGLEVMGIYD GYLGLYEDRM VQLDRYSVSD 

        70         80         90        100        110        120 
MINRGGTFLG SARFPEFRDE NIRAVAIENL KKRGIDALVV IGGDGSYMGA MRLTEMGFPC 

       130        140        150        160        170        180 
IGLPGTIDND IKGTDYTIGF FTALSTVVEA IDRLRDTSSS HQRISVVEVM GRYCGDLTLA 

       190        200        210        220        230        240 
AAIAGGCEFV VVPEVEFSRE DLVNEIKAGI AKGKKHAIVA ITEHMCDVDE LAHFIEKETG 

       250        260        270        280        290        300 
RETRATVLGH IQRGGSPVPY DRILASRMGA YAIDLLLAGY GGRCVGIQNE QLVHHDIIDA 

       310        320 
IENMKRPFKG DWLDCAKKLY

« Hide

References

« Hide 'large scale' references
[1]"The draft genome of Escherichia coli W."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Land M.L., Hauser L., Chang Y.-J., Jeffries C., Tremaine M., Landick R., Keating D., Woyke T.J.
Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: W EMBL EFN35972.1.
[2]"The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli."
Archer C.T., Kim J.F., Jeong H., Park J.H., Vickers C.E., Lee S.Y., Nielsen L.K.
BMC Genomics 12:9-9(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9637 / CCM 2024 / DSM 1116 / NCIMB 8666 / NRRL B-766 / W and W EMBL ADT77574.1.
[3]"Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilis pdc and adhB genes."
Turner P.C., Yomano L.P., Jarboe L.R., York S.W., Baggett C.L., Moritz B.E., Zentz E.B., Shanmugam K.T., Ingram L.O.
J. Ind. Microbiol. Biotechnol. 39:629-639(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9637 / CCM 2024 / DSM 1116 / NCIMB 8666 / NRRL B-766 / W and W EMBL AFH13804.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002185 Genomic DNA. Translation: ADT77574.1.
CP002967 Genomic DNA. Translation: AFH13804.1.
AEDF01000043 Genomic DNA. Translation: EFN35972.1.
RefSeqYP_006126816.1. NC_017635.1.
YP_006175590.1. NC_017664.1.

3D structure databases

SMRE0J788. Positions 1-320.
ModBaseSearch...

Proteomic databases

PRIDEE0J788.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADT77574; ADT77574; ECW_m4268.
AFH13804; AFH13804; WFL_20810.
EFN35972; EFN35972; EschWDRAFT_4446.
GeneID12698019.
12752567.
KEGGell:WFL_20810.
elw:ECW_m4268.
PATRIC41598376. VBIEscCol201000_4867.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00850.

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. Ppfruckinase. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE0J788_ECOLW
AccessionPrimary (citable) accession number: E0J788
Entry history
Integrated into UniProtKB/TrEMBL: November 2, 2010
Last sequence update: November 2, 2010
Last modified: May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info