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Protein

Apyrase

Gene
N/A
Organism
Aedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates hematophagy by inhibiting ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood. Platelet aggregation was inhibited by 6% when 0.4 µM recombinant apyrase was added and by 9.5% when the concentration of recombinant apyrase was 0.8 µM.1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

a divalent metal cationBy similarity

Kineticsi

  1. KM=11.6 µM for the recombinant enzyme1 Publication
  1. Vmax=1.02 nmol/sec/µg enzyme toward ATP1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Divalent metal cation 1By similarity1
Metal bindingi50Divalent metal cation 1By similarity1
Metal bindingi99Divalent metal cation 1By similarity1
Metal bindingi99Divalent metal cation 2By similarity1
Metal bindingi131Divalent metal cation 2By similarity1
Sitei132Transition state stabilizerBy similarity1
Sitei135Transition state stabilizerBy similarity1
Metal bindingi234Divalent metal cation 2By similarity1
Metal bindingi258Divalent metal cation 2By similarity1
Binding sitei425SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.1.5. 8486.

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase1 Publication (EC:3.6.1.51 Publication)
Alternative name(s):
ATP-diphosphataseBy similarity
Short name:
ADPaseBy similarity
ATP-diphosphohydrolaseBy similarity
Adenosine diphosphatase1 PublicationBy similarity
Allergen: Aed al 11 Publication
OrganismiAedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Taxonomic identifieri7160 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000069940 Componenti: Genome

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei1345. Aed al 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_500062395026 – 564Apyrase1 PublicationAdd BLAST539

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi391N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Salivary gland specific.2 Publications

Structurei

3D structure databases

ProteinModelPortaliE0D877.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni509 – 515Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Sequence analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E0D877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKPGIQLF VIFLLLSSFA AVVWAMDNMP ADKDVSKLFP LTLIHINDLH
60 70 80 90 100
ARFDETNMKS NACTAKDQCI AGIARVYQKI QDLLKEYKSK NAIYLNAGDN
110 120 130 140 150
FQGTLWYNLL RWQVTADFIT KLKPTAMTLG NHEFDHTPKG LAPYLAELDK
160 170 180 190 200
AGIPTLVANL VMNDDPDLKS SKIQKSIKVT VGGKTIGIIG VLYDKTHEIA
210 220 230 240 250
QTGKVTLSNA VETVKREAAA LKKDKVDIIV VLSHCSYDED KKIAKEAGQD
260 270 280 290 300
IDVIVGAHSH SFLYSKESNK PYDQKDKIEG PYPTIVESNN KRKIPIVQAK
310 320 330 340 350
SFGKYVGRLT LYFDNEGEVK HWEGYPEFID NKVKQDPKIL EALIPWRKKV
360 370 380 390 400
QEIGSTKVGE TTIELDRDSC RDKECTLGVL YADAFADHYT NSSFRPFAII
410 420 430 440 450
QAGNFRNPIK VGKITNGDII EAAPFGSTAD LIRLKGDNLW AVAEHSLALD
460 470 480 490 500
DENRTNCLQV SGLRIVIDPS KSVGSRVVKI DVMDNRNPKS EDLKPLDRNA
510 520 530 540 550
EYFIALPSYL ADGKDGFSAM KEATARWTGP LDSDVFKSYV EKIKKVDKLK
560
LDRVIVCKAG SPCT
Length:564
Mass (Da):62,751
Last modified:November 2, 2010 - v1
Checksum:i15B9F0F2D3BD0825
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14L → I in AAV90659 (PubMed:17244540).Curated1
Sequence conflicti25 – 26AM → TT in AAV90659 (PubMed:17244540).Curated2
Sequence conflicti26M → T AA sequence (PubMed:17244540).Curated1
Sequence conflicti438N → S in AAV90659 (PubMed:17244540).Curated1
Sequence conflicti447L → F in AAV90659 (PubMed:17244540).Curated1
Sequence conflicti472 – 473SV → KI in AAV90659 (PubMed:17244540).Curated2
Sequence conflicti498R → K in AAV90659 (PubMed:17244540).Curated1
Sequence conflicti522E → K in AAV90659 (PubMed:17244540).Curated1
Sequence conflicti551 – 552LD → WG in AAV90659 (PubMed:17244540).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB576780 mRNA. Translation: BAJ14796.1.
AY826087 mRNA. Translation: AAV90659.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB576780 mRNA. Translation: BAJ14796.1.
AY826087 mRNA. Translation: AAV90659.1.

3D structure databases

ProteinModelPortaliE0D877.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei1345. Aed al 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.6.1.5. 8486.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPY_AEDAL
AccessioniPrimary (citable) accession number: E0D877
Secondary accession number(s): Q5MIX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 2, 2010
Last modified: November 30, 2016
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.