Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

E0D877 (APY_AEDAL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apyrase

EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
Short name=ADPase
ATP-diphosphohydrolase
Adenosine diphosphatase
Allergen=Aed al 1
OrganismAedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Taxonomic identifier7160 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Facilitates hematophagy by inhibiting ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood. Platelet aggregation was inhibited by 6% when 0.4 µM recombinant apyrase was added and by 9.5% when the concentration of recombinant apyrase was 0.8 µM. Ref.1

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Divalent metal cations By similarity. UniProtKB Q9USP2

Subcellular location

Secreted Ref.1.

Tissue specificity

Salivary gland specific. Ref.1 Ref.2

Allergenic properties

Causes an allergic reaction in human. Ref.2

Sequence similarities

Belongs to the 5'-nucleotidase family.

Biophysicochemical properties

Kinetic parameters:

KM=11.6 µM for the recombinant enzyme Ref.1

Vmax=1.02 nmol/sec/µg enzyme toward ATP Ref.1

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   DomainSignal
   LigandATP-binding
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processnucleotide catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity, acting on ester bonds

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.2
Chain26 – 564539Apyrase Ref.1
PRO_5000623950

Regions

Region509 – 5157Substrate binding By similarity UniProtKB P50635

Sites

Metal binding481Divalent metal cation 1 By similarity UniProtKB P50635
Metal binding501Divalent metal cation 1 By similarity UniProtKB P50635
Metal binding991Divalent metal cation 1 By similarity UniProtKB P50635
Metal binding991Divalent metal cation 2 By similarity UniProtKB P50635
Metal binding1311Divalent metal cation 2 By similarity UniProtKB P50635
Metal binding2341Divalent metal cation 2 By similarity UniProtKB P50635
Metal binding2581Divalent metal cation 2 By similarity UniProtKB P50635
Binding site4251Substrate By similarity UniProtKB P50635
Site1321Transition state stabilizer By similarity UniProtKB P50635
Site1351Transition state stabilizer By similarity UniProtKB P50635

Amino acid modifications

Glycosylation3911N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict141L → I in AAV90659. Ref.2
Sequence conflict25 – 262AM → TT in AAV90659. Ref.2
Sequence conflict261M → T AA sequence Ref.2
Sequence conflict4381N → S in AAV90659. Ref.2
Sequence conflict4471L → F in AAV90659. Ref.2
Sequence conflict472 – 4732SV → KI in AAV90659. Ref.2
Sequence conflict4981R → K in AAV90659. Ref.2
Sequence conflict5221E → K in AAV90659. Ref.2
Sequence conflict551 – 5522LD → WG in AAV90659. Ref.2

Sequences

Sequence LengthMass (Da)Tools
E0D877 [UniParc].

Last modified November 2, 2010. Version 1.
Checksum: 15B9F0F2D3BD0825

FASTA56462,751
        10         20         30         40         50         60 
MAGKPGIQLF VIFLLLSSFA AVVWAMDNMP ADKDVSKLFP LTLIHINDLH ARFDETNMKS 

        70         80         90        100        110        120 
NACTAKDQCI AGIARVYQKI QDLLKEYKSK NAIYLNAGDN FQGTLWYNLL RWQVTADFIT 

       130        140        150        160        170        180 
KLKPTAMTLG NHEFDHTPKG LAPYLAELDK AGIPTLVANL VMNDDPDLKS SKIQKSIKVT 

       190        200        210        220        230        240 
VGGKTIGIIG VLYDKTHEIA QTGKVTLSNA VETVKREAAA LKKDKVDIIV VLSHCSYDED 

       250        260        270        280        290        300 
KKIAKEAGQD IDVIVGAHSH SFLYSKESNK PYDQKDKIEG PYPTIVESNN KRKIPIVQAK 

       310        320        330        340        350        360 
SFGKYVGRLT LYFDNEGEVK HWEGYPEFID NKVKQDPKIL EALIPWRKKV QEIGSTKVGE 

       370        380        390        400        410        420 
TTIELDRDSC RDKECTLGVL YADAFADHYT NSSFRPFAII QAGNFRNPIK VGKITNGDII 

       430        440        450        460        470        480 
EAAPFGSTAD LIRLKGDNLW AVAEHSLALD DENRTNCLQV SGLRIVIDPS KSVGSRVVKI 

       490        500        510        520        530        540 
DVMDNRNPKS EDLKPLDRNA EYFIALPSYL ADGKDGFSAM KEATARWTGP LDSDVFKSYV 

       550        560 
EKIKKVDKLK LDRVIVCKAG SPCT 

« Hide

References

[1]"Cloning, expression, and characterization of salivary apyrase from Aedes albopictus."
Dong F., Fu Y., Li X., Jiang J., Sun J., Cheng X.
Parasitol. Res. 110:931-937(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Salivary gland.
[2]"An insight into the sialome of the adult female mosquito Aedes albopictus."
Arca B., Lombardo F., Francischetti I.M., Pham V.M., Mestres-Simon M., Andersen J.F., Ribeiro J.M.
Insect Biochem. Mol. Biol. 37:107-127(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-45, TISSUE SPECIFICITY, ALLERGEN.
Tissue: Salivary gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB576780 mRNA. Translation: BAJ14796.1.
AY826087 mRNA. Translation: AAV90659.1.

3D structure databases

ProteinModelPortalE0D877.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome1345. Aed al 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERPTHR11575. PTHR11575. 1 hit.
PfamPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR01607. APYRASEFAMLY.
SUPFAMSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPY_AEDAL
AccessionPrimary (citable) accession number: E0D877
Secondary accession number(s): Q5MIX3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 2, 2010
Last modified: June 11, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families