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Protein

Apyrase

Gene
N/A
Organism
Aedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates hematophagy by inhibiting ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood. Platelet aggregation was inhibited by 6% when 0.4 µM recombinant apyrase was added and by 9.5% when the concentration of recombinant apyrase was 0.8 µM.1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

a divalent metal cationBy similarity

Kineticsi

  1. KM=11.6 µM for the recombinant enzyme1 Publication

Vmax=1.02 nmol/sec/µg enzyme toward ATP1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Divalent metal cation 1By similarity
Metal bindingi50 – 501Divalent metal cation 1By similarity
Metal bindingi99 – 991Divalent metal cation 1By similarity
Metal bindingi99 – 991Divalent metal cation 2By similarity
Metal bindingi131 – 1311Divalent metal cation 2By similarity
Sitei132 – 1321Transition state stabilizerBy similarity
Sitei135 – 1351Transition state stabilizerBy similarity
Metal bindingi234 – 2341Divalent metal cation 2By similarity
Metal bindingi258 – 2581Divalent metal cation 2By similarity
Binding sitei425 – 4251SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity, acting on ester bonds Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase1 Publication (EC:3.6.1.51 Publication)
Alternative name(s):
ATP-diphosphataseBy similarity
Short name:
ADPaseBy similarity
ATP-diphosphohydrolaseBy similarity
Adenosine diphosphatase1 PublicationBy similarity
Allergen: Aed al 11 Publication
OrganismiAedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Taxonomic identifieri7160 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei1345. Aed al 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 564539Apyrase1 PublicationPRO_5000623950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Salivary gland specific.2 Publications

Structurei

3D structure databases

ProteinModelPortaliE0D877.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni509 – 5157Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Sequence Analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E0D877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKPGIQLF VIFLLLSSFA AVVWAMDNMP ADKDVSKLFP LTLIHINDLH
60 70 80 90 100
ARFDETNMKS NACTAKDQCI AGIARVYQKI QDLLKEYKSK NAIYLNAGDN
110 120 130 140 150
FQGTLWYNLL RWQVTADFIT KLKPTAMTLG NHEFDHTPKG LAPYLAELDK
160 170 180 190 200
AGIPTLVANL VMNDDPDLKS SKIQKSIKVT VGGKTIGIIG VLYDKTHEIA
210 220 230 240 250
QTGKVTLSNA VETVKREAAA LKKDKVDIIV VLSHCSYDED KKIAKEAGQD
260 270 280 290 300
IDVIVGAHSH SFLYSKESNK PYDQKDKIEG PYPTIVESNN KRKIPIVQAK
310 320 330 340 350
SFGKYVGRLT LYFDNEGEVK HWEGYPEFID NKVKQDPKIL EALIPWRKKV
360 370 380 390 400
QEIGSTKVGE TTIELDRDSC RDKECTLGVL YADAFADHYT NSSFRPFAII
410 420 430 440 450
QAGNFRNPIK VGKITNGDII EAAPFGSTAD LIRLKGDNLW AVAEHSLALD
460 470 480 490 500
DENRTNCLQV SGLRIVIDPS KSVGSRVVKI DVMDNRNPKS EDLKPLDRNA
510 520 530 540 550
EYFIALPSYL ADGKDGFSAM KEATARWTGP LDSDVFKSYV EKIKKVDKLK
560
LDRVIVCKAG SPCT
Length:564
Mass (Da):62,751
Last modified:November 2, 2010 - v1
Checksum:i15B9F0F2D3BD0825
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141L → I in AAV90659 (PubMed:17244540).Curated
Sequence conflicti25 – 262AM → TT in AAV90659 (PubMed:17244540).Curated
Sequence conflicti26 – 261M → T AA sequence (PubMed:17244540).Curated
Sequence conflicti438 – 4381N → S in AAV90659 (PubMed:17244540).Curated
Sequence conflicti447 – 4471L → F in AAV90659 (PubMed:17244540).Curated
Sequence conflicti472 – 4732SV → KI in AAV90659 (PubMed:17244540).Curated
Sequence conflicti498 – 4981R → K in AAV90659 (PubMed:17244540).Curated
Sequence conflicti522 – 5221E → K in AAV90659 (PubMed:17244540).Curated
Sequence conflicti551 – 5522LD → WG in AAV90659 (PubMed:17244540).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB576780 mRNA. Translation: BAJ14796.1.
AY826087 mRNA. Translation: AAV90659.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB576780 mRNA. Translation: BAJ14796.1.
AY826087 mRNA. Translation: AAV90659.1.

3D structure databases

ProteinModelPortaliE0D877.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei1345. Aed al 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, expression, and characterization of salivary apyrase from Aedes albopictus."
    Dong F., Fu Y., Li X., Jiang J., Sun J., Cheng X.
    Parasitol. Res. 110:931-937(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Salivary glandImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-45, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Salivary glandImported.

Entry informationi

Entry nameiAPY_AEDAL
AccessioniPrimary (citable) accession number: E0D877
Secondary accession number(s): Q5MIX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 2, 2010
Last modified: January 7, 2015
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.