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E0D877

- APY_AEDAL

UniProt

E0D877 - APY_AEDAL

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Protein
Apyrase
Gene
N/A
Organism
Aedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Facilitates hematophagy by inhibiting ADP-dependent platelet aggregation in the host. May reduce probing time by facilitating the speed of locating blood. Platelet aggregation was inhibited by 6% when 0.4 µM recombinant apyrase was added and by 9.5% when the concentration of recombinant apyrase was 0.8 µM.1 Publication

Catalytic activityi

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactori

Divalent metal cations By similarity.By similarity

Kineticsi

  1. KM=11.6 µM for the recombinant enzyme1 Publication

Vmax=1.02 nmol/sec/µg enzyme toward ATP1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Divalent metal cation 1 By similarityBy similarity
Metal bindingi50 – 501Divalent metal cation 1 By similarityBy similarity
Metal bindingi99 – 991Divalent metal cation 1 By similarityBy similarity
Metal bindingi99 – 991Divalent metal cation 2 By similarityBy similarity
Metal bindingi131 – 1311Divalent metal cation 2 By similarityBy similarity
Sitei132 – 1321Transition state stabilizer By similarityBy similarity
Sitei135 – 1351Transition state stabilizer By similarityBy similarity
Metal bindingi234 – 2341Divalent metal cation 2 By similarityBy similarity
Metal bindingi258 – 2581Divalent metal cation 2 By similarityBy similarity
Binding sitei425 – 4251Substrate By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity, acting on ester bonds Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apyrase (EC:3.6.1.5)
Alternative name(s):
ATP-diphosphatase
Short name:
ADPase
ATP-diphosphohydrolase
Adenosine diphosphatase
Allergen: Aed al 1
OrganismiAedes albopictus (Asian tiger mosquito) (Stegomyia albopicta)
Taxonomic identifieri7160 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei1345. Aed al 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 Publication
Add
BLAST
Chaini26 – 564539Apyrase1 Publication
PRO_5000623950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi391 – 3911N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Salivary gland specific.2 Publications

Structurei

3D structure databases

ProteinModelPortaliE0D877.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni509 – 5157Substrate binding By similarityBy similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

E0D877-1 [UniParc]FASTAAdd to Basket

« Hide

MAGKPGIQLF VIFLLLSSFA AVVWAMDNMP ADKDVSKLFP LTLIHINDLH    50
ARFDETNMKS NACTAKDQCI AGIARVYQKI QDLLKEYKSK NAIYLNAGDN 100
FQGTLWYNLL RWQVTADFIT KLKPTAMTLG NHEFDHTPKG LAPYLAELDK 150
AGIPTLVANL VMNDDPDLKS SKIQKSIKVT VGGKTIGIIG VLYDKTHEIA 200
QTGKVTLSNA VETVKREAAA LKKDKVDIIV VLSHCSYDED KKIAKEAGQD 250
IDVIVGAHSH SFLYSKESNK PYDQKDKIEG PYPTIVESNN KRKIPIVQAK 300
SFGKYVGRLT LYFDNEGEVK HWEGYPEFID NKVKQDPKIL EALIPWRKKV 350
QEIGSTKVGE TTIELDRDSC RDKECTLGVL YADAFADHYT NSSFRPFAII 400
QAGNFRNPIK VGKITNGDII EAAPFGSTAD LIRLKGDNLW AVAEHSLALD 450
DENRTNCLQV SGLRIVIDPS KSVGSRVVKI DVMDNRNPKS EDLKPLDRNA 500
EYFIALPSYL ADGKDGFSAM KEATARWTGP LDSDVFKSYV EKIKKVDKLK 550
LDRVIVCKAG SPCT 564
Length:564
Mass (Da):62,751
Last modified:November 2, 2010 - v1
Checksum:i15B9F0F2D3BD0825
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141L → I in AAV90659. 1 Publication
Sequence conflicti25 – 262AM → TT in AAV90659. 1 Publication
Sequence conflicti26 – 261M → T AA sequence 1 Publication
Sequence conflicti438 – 4381N → S in AAV90659. 1 Publication
Sequence conflicti447 – 4471L → F in AAV90659. 1 Publication
Sequence conflicti472 – 4732SV → KI in AAV90659. 1 Publication
Sequence conflicti498 – 4981R → K in AAV90659. 1 Publication
Sequence conflicti522 – 5221E → K in AAV90659. 1 Publication
Sequence conflicti551 – 5522LD → WG in AAV90659. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB576780 mRNA. Translation: BAJ14796.1.
AY826087 mRNA. Translation: AAV90659.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB576780 mRNA. Translation: BAJ14796.1 .
AY826087 mRNA. Translation: AAV90659.1 .

3D structure databases

ProteinModelPortali E0D877.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 1345. Aed al 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression, and characterization of salivary apyrase from Aedes albopictus."
    Dong F., Fu Y., Li X., Jiang J., Sun J., Cheng X.
    Parasitol. Res. 110:931-937(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Salivary gland.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-45, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Salivary gland.

Entry informationi

Entry nameiAPY_AEDAL
AccessioniPrimary (citable) accession number: E0D877
Secondary accession number(s): Q5MIX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 2, 2010
Last modified: June 11, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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