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Protein

Probable DNA ligase

Gene

lig

Organism
Micromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / JCM 10878 / NBRC 16125 / INA 9442)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei209 – 2091ATPUniRule annotation
Active sitei211 – 2111N6-AMP-lysine intermediateUniRule annotation
Binding sitei216 – 2161ATPUniRule annotation
Binding sitei231 – 2311ATPUniRule annotation
Binding sitei260 – 2601ATPUniRule annotation
Binding sitei315 – 3151ATPUniRule annotation
Binding sitei387 – 3871ATPUniRule annotation
Binding sitei393 – 3931ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Cell cycle, Cell divisionUniRule annotation, DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMAUR644283:GHOD-5010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:Micau_4966Imported
OrganismiMicromonospora aurantiaca (strain ATCC 27029 / DSM 43813 / JCM 10878 / NBRC 16125 / INA 9442)Imported
Taxonomic identifieri644283 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeMicromonospora
ProteomesiUP000001908 Componenti: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000036008.
KOiK10747.
OMAiGRPRPFQ.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 2 hits.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D9TAL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFAELAATS AAVGATSGRR AKVELLAAAL RGLEPGEIPA GAGYLAGELR
60 70 80 90 100
QRQTGVGWAG LRDLPPPAAE PTLTVAAVDA AIEEISRVAG AGSQARRREL
110 120 130 140 150
VGRLFAAATA DEQRLLTGLF RGELRQGAQA GLLADAVARA ADVPVTAVRR
160 170 180 190 200
ALLLAGDLRA VAVAALTGGA AALAGFGLQV GRPLAPMLAQ SAPSVDEALT
210 220 230 240 250
ATGVPAMVDV KLDGIRIQVH RSGPDIAVFT RSLDEITGRL PQVVAAVRAL
260 270 280 290 300
PARELVLDGE AIGLDAAGRP LPFQETSSRA ARRTTPSTTG QAPVAPAVLA
310 320 330 340 350
AAERTGEPVL TPYFFDLLHL DGTDLIDAPG RERWAALAGA VDASLLVDRI
360 370 380 390 400
EVDDVEQAAT AFAAAVDAGQ EGVVVKDPAA PYDAGRRGSA WVKVKPRHTL
410 420 430 440 450
DLVVLAVEWG SGRREGWLSN LHLGARDPRT GEFVMLGKTF KGLTDELLRW
460 470 480 490 500
QTERFLSLAV EKGDWVVRVR PEQVVEIAFD GVQTSSRYPG GMALRFARVL
510 520
RYRDDKTAAE ADTIDAVRAI HAGRVTG
Length:527
Mass (Da):55,322
Last modified:October 5, 2010 - v1
Checksum:i85C36A68BE3D5D71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002162 Genomic DNA. Translation: ADL48475.1.
RefSeqiYP_003838051.1. NC_014391.1.

Genome annotation databases

EnsemblBacteriaiADL48475; ADL48475; Micau_4966.
KEGGimau:Micau_4966.
PATRICi42395721. VBIMicAur74833_5000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002162 Genomic DNA. Translation: ADL48475.1.
RefSeqiYP_003838051.1. NC_014391.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL48475; ADL48475; Micau_4966.
KEGGimau:Micau_4966.
PATRICi42395721. VBIMicAur74833_5000.

Phylogenomic databases

HOGENOMiHOG000036008.
KOiK10747.
OMAiGRPRPFQ.

Enzyme and pathway databases

BioCyciMAUR644283:GHOD-5010-MONOMER.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 2 hits.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Micromonospora aurantiaca ATCC 27029."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G., Hirsch A.M., Woyke T.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27029 / DSM 43813 / JCM 10878 / NBRC 16125 / INA 9442Imported.

Entry informationi

Entry nameiD9TAL9_MICAI
AccessioniPrimary (citable) accession number: D9TAL9
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: April 1, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.