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Protein

Pyrophosphate--fructose 6-phosphate 1-phosphotransferase

Gene

pfp

Organism
Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.UniRule annotation

Catalytic activityi

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Non-allosteric.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Diphosphate; via amide nitrogenUniRule annotation
Metal bindingi111 – 1111Magnesium; catalyticUniRule annotation
Sitei112 – 1121Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATPUniRule annotation
Sitei138 – 1381Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPiUniRule annotation
Active sitei141 – 1411Proton acceptorUniRule annotation
Binding sitei242 – 2421SubstrateUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: InterPro

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciCCEL573061:GIXD-1662-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferaseUniRule annotation (EC:2.7.1.90UniRule annotation)
Alternative name(s):
6-phosphofructokinase, pyrophosphate dependentUniRule annotation
PPi-dependent phosphofructokinaseUniRule annotation
Pyrophosphate-dependent 6-phosphofructose-1-kinaseUniRule annotation
Gene namesi
Name:pfpUniRule annotation
Ordered Locus Names:Clocel_1603Imported
OrganismiClostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)Imported
Taxonomic identifieri573061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000002730 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD9SWY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni139 – 1413Substrate bindingUniRule annotation
Regioni185 – 1873Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade "B2" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000007357.
KOiK00850.
OMAiCGVIETA.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

D9SWY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNCIVAQSG GPTAVINSSV IGVFYENLKV KYYDNVYSGL NGIEGILKGD
60 70 80 90 100
IINLSELEYD KIDTLKYTPA SALGSCRYKI DDYKENDEDY NNLFKILNSL
110 120 130 140 150
EIQTFFYVGG NDSMDTVSKL STYAQKCGID INFIGIPKTI DNDLCLTDHT
160 170 180 190 200
PGYGSAAKFI ATTALETYLD SSVYTNNGIF ILETMGRDTG WLAASAALAI
210 220 230 240 250
IDDRMIADFI YLPEVAFDID NFLSDVKERF IANNQVYIVV SEGIKDKNGN
260 270 280 290 300
FISETKIANN HDNFGHNQLG GVGNVLKELI INAGITNRVK TLELGVMQRC
310 320 330 340 350
AMHCASLTDL EEAHTVGRSA VSFSISGATG KMVGIKRVNE TPYLYDTELV
360 370 380 390 400
DVKQVANKIK YVPTDWINTE GNHVNEKAIN YIRPLIQGIP NLDTKNGLPK
410
YVMVEKDLVS
Length:410
Mass (Da):45,301
Last modified:October 5, 2010 - v1
Checksum:i0FA0E9D6E7719816
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002160 Genomic DNA. Translation: ADL51350.1.
RefSeqiWP_010077442.1. NZ_BABR01000420.1.
YP_003843114.1. NC_014393.1.

Genome annotation databases

EnsemblBacteriaiADL51350; ADL51350; Clocel_1603.
KEGGiccb:Clocel_1603.
PATRICi41741114. VBICloCel81632203721_4248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002160 Genomic DNA. Translation: ADL51350.1.
RefSeqiWP_010077442.1. NZ_BABR01000420.1.
YP_003843114.1. NC_014393.1.

3D structure databases

ProteinModelPortaliD9SWY9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL51350; ADL51350; Clocel_1603.
KEGGiccb:Clocel_1603.
PATRICi41741114. VBICloCel81632203721_4248.

Phylogenomic databases

HOGENOMiHOG000007357.
KOiK00850.
OMAiCGVIETA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciCCEL573061:GIXD-1662-MONOMER.

Family and domain databases

HAMAPiMF_01978. Phosphofructokinase_II_B2.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR011404. PPi-PFK_XF0274.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF036483. PFK_XF0274. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35296 / DSM 3052 / OCM 3 / 743BImported.

Entry informationi

Entry nameiD9SWY9_CLOC7
AccessioniPrimary (citable) accession number: D9SWY9
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: April 29, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.