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D9SM34

- BGAL_CLOC7

UniProt

D9SM34 - BGAL_CLOC7

Protein

Beta-galactosidase BgaA

Gene

bgaA

Organism
Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Involved in plant cell wall degradation in cooperation with cellulosome. Hydrolyzes both p-nitrophenyl-alpha-L-arabinopyranoside (pNPAp) and p-nitrophenyl-beta-D-galactopyranoside (pNPGp), with higher activity for pNPAp. Shows hydrolysis activity against p-nitrophenyl-beta-D-fucopyranoside (pNPFp), but not against p-nitrophenyl-alpha-L-arabinofuranoside (pNPAf), o-nitrophenyl-beta-D-galactopyranoside (oNPGp), p-nitrophenyl-beta-D-xylopyranoside (pNPXp), p-nitrophenyl-beta-D-glucopyranoside (pNPGLp), p-nitrophenyl-beta-D-cellobiopyranoside (pNPCp), p-nitrophenyl-beta-lactopyranoside (pNPLp) or p-nitrophenyl-alpha-galactopyranoside (pNPalphaGp). No detectable activity against arabinan or arabinoxylan, but activity against arabinogalactan can be detected. Increases degradation activity of alpha-L-arabinofuranosidase (ArfA) and endo-1,4-beta-xylanase (XynA) when corn fiber gum and corn stem powder are used as substrates.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Enzyme regulationi

    Inhibited by Cu2+, Hg2+ and Zn2+. No effect with Ca2+, Mg2+, Mn2+ or excess EDTA (10 mM).1 Publication

    Kineticsi

    1. KM=1.51 mM for pNPAp (at 37 degrees Celsius and pH 6.0)1 Publication
    2. KM=6.06 mM for pNPGp (at 37 degrees Celsius and pH 6.0)1 Publication

    Vmax=10.4 µmol/min/mg enzyme with pNPAp as substrate (at 37 degrees Celsius and pH 6.0)1 Publication

    Vmax=2.5 µmol/min/mg enzyme with pNPGp as substrate (at 37 degrees Celsius and pH 6.0)1 Publication

    pH dependencei

    Optimum pH is 6.0 for activities against both pNPAp and pNPGp. Stable in the range of pH 6.0-8.0.1 Publication

    Temperature dependencei

    Optimum temperature is 30-40 degrees Celsius for activities against both pNPAp and pNPGp when incubated 10 minutes at pH 6.0. Both activities completely lost after heating at 50 degrees Celsius for 20 minutes.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031SubstrateBy similarity
    Metal bindingi107 – 1071ZincBy similarity
    Binding sitei141 – 1411SubstrateBy similarity
    Active sitei142 – 1421Proton donorBy similarity
    Metal bindingi148 – 1481ZincBy similarity
    Metal bindingi150 – 1501ZincBy similarity
    Metal bindingi153 – 1531ZincBy similarity
    Active sitei298 – 2981NucleophileBy similarity
    Binding sitei307 – 3071SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciCCEL573061:GIXD-2086-MONOMER.

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BgaA (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Alternative name(s):
    Alpha-L-arabinopyranosidase1 Publication
    Gene namesi
    Name:bgaA
    Ordered Locus Names:Clocel_2022
    OrganismiClostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B)
    Taxonomic identifieri573061 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000002730: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 659659Beta-galactosidase BgaAPRO_0000407687Add
    BLAST

    Interactioni

    Subunit structurei

    Dimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliD9SM34.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Phylogenomic databases

    HOGENOMiHOG000117811.
    KOiK12308.
    OMAiIGYLPRP.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D9SM34-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIGVDYYPE HWDRQLWEKD AQLMKEIGVK VVRLAEFAWC KLEPIEGQYD    50
    FKWLDDVIEI FSVRNIEIVL GTPTNTPPLW LYEKYPDAIQ VNESGERQFI 100
    GIRGHRCYNS SSMRKYTKAI VEAMTERYAN NKAVIGWQID NELDATHCCC 150
    DNCTEKFRGW LKNKYSTLEN INKEYGNVVW SGEYSAWSQV TAPLGGSPFL 200
    NPSYLLDYNR FASDSMVEYI DFQREIIRKN CPSQFITTNT WFTGNLPNFY 250
    DAFENLDFVS YDNYPTTNEI TDEEELHSHA FHCDLMRGIK KKNFWIMEQL 300
    SGTPGCWMPM QRTPKPGMIK GYSFQAIGRG AETVVHFRWR NAIIGAEMFW 350
    HGILDHSNVK GRRFYEFAEL CREVNKINEE IPDYKINNEV AILYSSDQDF 400
    AFKIQPQVEG LYYLQQLKAF HNALIRLGVG TDIINWSESL NKYKVVIAPT 450
    LYLTDDNVTT ELYRFVEAGG TLILTNRTGV KNMNNVCLME QMPSNLKECA 500
    GVVVKEYDPI GHSIHTIKDE AGKVYQCKQW CDILEPTTAK VIATYNDDFY 550
    IDEAAVTVNK YKKGNVYYLG TVFNSDYYIE LLSKILDEKE LPYYKKLPYG 600
    LELSVLENEN GKYLMVFNNS NEIKCFEGKH EGKSIIRNEL DGKSFTLEPY 650
    GIEVLQLVE 659
    Length:659
    Mass (Da):76,464
    Last modified:October 5, 2010 - v1
    Checksum:i30C0DB5C33848BF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY128945 Genomic DNA. Translation: AAN05452.1.
    CP002160 Genomic DNA. Translation: ADL51765.1.
    RefSeqiWP_010077016.1. NZ_BABR01000402.1.
    YP_003843529.1. NC_014393.1.

    Genome annotation databases

    EnsemblBacteriaiADL51765; ADL51765; Clocel_2022.
    GeneIDi9608894.
    KEGGiccb:Clocel_2022.
    PATRICi41740234. VBICloCel81632203721_3817.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY128945 Genomic DNA. Translation: AAN05452.1 .
    CP002160 Genomic DNA. Translation: ADL51765.1 .
    RefSeqi WP_010077016.1. NZ_BABR01000402.1.
    YP_003843529.1. NC_014393.1.

    3D structure databases

    ProteinModelPortali D9SM34.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL51765 ; ADL51765 ; Clocel_2022 .
    GeneIDi 9608894.
    KEGGi ccb:Clocel_2022.
    PATRICi 41740234. VBICloCel81632203721_3817.

    Phylogenomic databases

    HOGENOMi HOG000117811.
    KOi K12308.
    OMAi IGYLPRP.

    Enzyme and pathway databases

    BioCyci CCEL573061:GIXD-2086-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation."
      Kosugi A., Murashima K., Doi R.H.
      J. Bacteriol. 184:6859-6865(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-5, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY, SUBUNIT.
      Strain: ATCC 35296 / DSM 3052 / OCM 3 / 743B.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35296 / DSM 3052 / OCM 3 / 743BImported.

    Entry informationi

    Entry nameiBGAL_CLOC7
    AccessioniPrimary (citable) accession number: D9SM34
    Secondary accession number(s): Q8GEE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3