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D9R8A9 (D9R8A9_CLOSW) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Closa_1258 EMBL ADL03861.1
OrganismClostridium saccharolyticum (strain ATCC 35040 / DSM 2544 / NRCC 2533 / WM1) [Complete proteome] [HAMAP] EMBL ADL03861.1
Taxonomic identifier610130 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain142 – 333192ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding168 – 21952ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2871Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3001Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3021Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D9R8A9 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: 346E4ECB574B1F4F

FASTA35138,436
        10         20         30         40         50         60 
MKIVVLAGGF SPEREVSLSS GAMITNALIR TGHEVYLLDS YLGAADGEEV RFKSLHDGTG 

        70         80         90        100        110        120 
FFWEIGREVP ELDRLSKERN GKGYIGNRVI EICGLADIVF VALHGGAGEN GQIQAVLDAY 

       130        140        150        160        170        180 
GISYTGTGYE GCLKAMDKPM AKLLMRNSGI PTPDWRLYSR GESLEPFPYP CVVKPCGCGS 

       190        200        210        220        230        240 
SVGITMVEGE DQWEMALDSA FSYEDRVLAE VKITGREFSV GILGERALPA IEIIPKKGFY 

       250        260        270        280        290        300 
DYENKYQAGL TEEICPASLT EEQEEFMGCM ALKVHRVLGL GYYSRVDFLM EEDGSLFCLE 

       310        320        330        340        350 
ANTLPGMTPF SLLPQKAQAA GISYQELCEE IVSHGIHPAG VPLYPAKREH S 

« Hide

References

[1]"Complete sequence of Clostridium saccharolyticum WM1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Chang Y.-J., Jeffries C., Kyrpides N., Ivanova N., Mikhailova N. expand/collapse author list , Mouttaki H., Lin L., Zhou J., Hemme C.L., Woyke T.
Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35040 / DSM 2544 / NRCC 2533 / WM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002109 Genomic DNA. Translation: ADL03861.1.
RefSeqYP_003821484.1. NC_014376.1.

3D structure databases

ProteinModelPortalD9R8A9.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL03861; ADL03861; Closa_1258.
GeneID9506541.
KEGGcsh:Closa_1258.
PATRIC42263968. VBICloSac91065_1368.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.
OMAGCDARSS.

Enzyme and pathway databases

BioCycCSAC610130:GHTP-1304-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD9R8A9_CLOSW
AccessionPrimary (citable) accession number: D9R8A9
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)