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Protein
Submitted name:

F420-reducing hydrogenase, subunit gamma

Gene

frhG

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur(4Fe-4S) 1Combined sources
Metal bindingi56 – 561Iron-sulfur(4Fe-4S) 2Combined sources
Metal bindingi57 – 571Iron-sulfur(4Fe-4S) 1Combined sources
Metal bindingi59 – 591Iron-sulfur(4Fe-4S) 2Combined sources
Metal bindingi60 – 601Iron-sulfur(4Fe-4S) 1Combined sources
Metal bindingi131 – 1311Iron-sulfur(4Fe-4S) 1; via amide nitrogenCombined sources
Metal bindingi132 – 1321Iron-sulfur(4Fe-4S) 1Combined sources
Metal bindingi171 – 1711Iron-sulfur(4Fe-4S) 1Combined sources
Metal bindingi220 – 2201Iron-sulfur(4Fe-4S) 4Combined sources
Metal bindingi223 – 2231Iron-sulfur(4Fe-4S) 4Combined sources
Metal bindingi224 – 2241Iron-sulfur(4Fe-4S) 4; via amide nitrogenCombined sources
Metal bindingi224 – 2241Iron-sulfur(4Fe-4S) 5Combined sources
Metal bindingi225 – 2251Iron-sulfur(4Fe-4S) 4Combined sources
Metal bindingi226 – 2261Iron-sulfur(4Fe-4S) 4Combined sources
Metal bindingi230 – 2301Iron-sulfur(4Fe-4S) 3Combined sources
Metal bindingi232 – 2321Iron-sulfur(4Fe-4S) 3Combined sources
Metal bindingi235 – 2351Iron-sulfur(4Fe-4S) 3Combined sources
Metal bindingi249 – 2491Iron-sulfur(4Fe-4S) 3Combined sources
Metal bindingi251 – 2511Iron-sulfur(4Fe-4S) 7Combined sources
Metal bindingi252 – 2521Iron-sulfur(4Fe-4S) 3Combined sources
Metal bindingi255 – 2551Iron-sulfur(4Fe-4S) 3Combined sources
Metal bindingi255 – 2551Iron-sulfur(4Fe-4S) 7Combined sources
Metal bindingi259 – 2591Iron-sulfur(4Fe-4S) 4Combined sources

GO - Molecular functioni

  1. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro
  3. iron-sulfur cluster binding Source: InterPro
  4. nickel cation binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseImported

Keywords - Ligandi

Metal-bindingCombined sources

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1690-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
F420-reducing hydrogenase, subunit gammaImported (EC:1.12.98.1Imported)
Gene namesi
Name:frhGImported
Ordered Locus Names:MTBMA_c16840Imported
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)Imported
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345: Chromosome

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFSelectron microscopy4.00B1-275[»]
4CI0electron microscopy3.36B1-275[»]
4OMFX-ray1.71G1-275[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000236993.
KOiK00443.
OMAiCPPSPEI.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamiPF13237. Fer4_10. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03294. FrhG. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D9PYF7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLIARIKRF LGLEAEAKRE EPEKEKSEPV GASKEEVEKV AEENAKPRIG
60 70 80 90 100
YIHLSGCTGD AMSLTENYDI LAELLTNMVD IVYGQTLVDL WEMPEMDLAL
110 120 130 140 150
VEGSVCLQDE HSLHELKELR EKAKLVCAFG SCAATGCFTR YSRGGQQAQP
160 170 180 190 200
SHESFVPIAD LIDVDLALPG CPPSPEIIAK TVVALLNNDM DYLQPMLDLA
210 220 230 240 250
GYTEACGCDL QTKVVNQGLC IGCGTCAMAC QTRALDMTNG RPELNSDRCI
260 270
KCGICYVQCP RSWWPEEQIK KELGL
Length:275
Mass (Da):30,238
Last modified:October 5, 2010 - v1
Checksum:i858E41DFB402030A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001710 Genomic DNA. Translation: ADL59255.1.
RefSeqiYP_003850568.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL59255; ADL59255; MTBMA_c16840.
GeneIDi9705395.
KEGGimmg:MTBMA_c16840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001710 Genomic DNA. Translation: ADL59255.1.
RefSeqiYP_003850568.1. NC_014408.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZFSelectron microscopy4.00B1-275[»]
4CI0electron microscopy3.36B1-275[»]
4OMFX-ray1.71G1-275[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL59255; ADL59255; MTBMA_c16840.
GeneIDi9705395.
KEGGimmg:MTBMA_c16840.

Phylogenomic databases

HOGENOMiHOG000236993.
KOiK00443.
OMAiCPPSPEI.

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1690-MONOMER.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamiPF13237. Fer4_10. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03294. FrhG. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Methanothermobacter marburgensis."
    Kaster A., Seedorf H., Goenrich M., Wiezer A., Liesegang H., Thauer R., Gottschalk G.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / MarburgImported.
  3. "De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy."
    Mills D.J., Vitt S., Strauss M., Shima S., Vonck J.
    Elife 2:e00218-e00218(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (4.00 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR(4FE-4S).
  4. "Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector."
    Allegretti M., Mills D.J., McMullan G., Kuhlbrandt W., Vonck J.
    Elife 3:e01963-e01963(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (3.36 ANGSTROMS) IN COMPLEX WITH IRON-SULFUR(4FE-4S).

Entry informationi

Entry nameiD9PYF7_METTM
AccessioniPrimary (citable) accession number: D9PYF7
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: January 7, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.