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D9PYF6

- D9PYF6_METTM

UniProt

D9PYF6 - D9PYF6_METTM

Protein
Submitted name:

F420-reducing hydrogenase, subunit beta

Gene

frhB

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (05 Oct 2010)
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei48 – 481FAD; via amide nitrogenImported
    Metal bindingi103 – 1031Iron-sulfur (4Fe-4S) 1
    Metal bindingi103 – 1031Iron-sulfur(4Fe-4S) 1Imported
    Metal bindingi103 – 1031Iron-sulfur(4Fe-4S) 2Imported
    Metal bindingi104 – 1041Iron-sulfur (4Fe-4S) 1
    Metal bindingi104 – 1041Iron-sulfur(4Fe-4S) 1Imported
    Metal bindingi104 – 1041Iron-sulfur(4Fe-4S) 2Imported
    Binding sitei133 – 1331FAD; via carbonyl oxygen
    Metal bindingi134 – 1341Iron-sulfur (4Fe-4S) 1
    Metal bindingi134 – 1341Iron-sulfur(4Fe-4S) 1Imported
    Metal bindingi134 – 1341Iron-sulfur(4Fe-4S) 2Imported
    Metal bindingi192 – 1921Iron-sulfur (4Fe-4S) 1
    Metal bindingi192 – 1921Iron-sulfur(4Fe-4S) 1Imported
    Metal bindingi195 – 1951Iron-sulfur (4Fe-4S) 1
    Metal bindingi195 – 1951Iron-sulfur(4Fe-4S) 1Imported
    Binding sitei198 – 1981FADImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi24 – 307FADImported
    Nucleotide bindingi73 – 775FADImported
    Nucleotide bindingi133 – 1353FADImported
    Nucleotide bindingi207 – 2104FADImported

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
    3. flavin adenine dinucleotide binding Source: InterPro
    4. nickel cation binding Source: InterPro

    Keywords - Molecular functioni

    OxidoreductaseImported

    Keywords - Ligandi

    FADImported, Flavoprotein, Metal-bindingImported, Nucleotide-bindingImported

    Enzyme and pathway databases

    BioCyciMMAR79929:GH5J-1689-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    F420-reducing hydrogenase, subunit betaImported (EC:1.12.98.1Imported)
    Gene namesi
    Name:frhBImported
    Ordered Locus Names:MTBMA_c16830Imported
    OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)Imported
    Taxonomic identifieri79929 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000000345: Chromosome

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZFSelectron microscopy4.00C1-281[»]
    4CI0electron microscopy3.36C1-281[»]
    4OMFX-ray1.71B1-281[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    HOGENOMiHOG000223731.
    KOiK00441.
    OMAiMENFPYN.

    Family and domain databases

    InterProiIPR007516. Co_F420_Hydgase/DH_bsu_N.
    IPR017679. Coenz_F420_hydrogenase_bsu.
    IPR007525. FrhB_FdhB_C.
    [Graphical view]
    PfamiPF04432. FrhB_FdhB_C. 1 hit.
    PF04422. FrhB_FdhB_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03289. frhB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D9PYF6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLGTYKEIV SARSTDREIQ KLAQDGGIVT GLLAYALDEG IIEGAVVAGP    50
    GEEFWKPQPM VAMSSDELKA AAGTKYTFSP NVMMLKKAVR QYGIEKLGTV 100
    AIPCQTMGIR KMQTYPFGVR FLADKIKLLV GIYCMENFPY TSLQTFICEK 150
    LGVSMELVEK MDIGKGKFWV YTQDDVLTLP LKETHGYEQA GCKICKDYVA 200
    ELADVSTGSV GSPDGWSTVI TRTDAGDSIF KQAVEAGLFE TKPIEEVKPG 250
    LGLLEKLAAQ KKEKAEKNIA ARKEMGLPTP F 281
    Length:281
    Mass (Da):30,744
    Last modified:October 5, 2010 - v1
    Checksum:i1E981B4ADF3B1A16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001710 Genomic DNA. Translation: ADL59254.1.
    RefSeqiYP_003850567.1. NC_014408.1.

    Genome annotation databases

    EnsemblBacteriaiADL59254; ADL59254; MTBMA_c16830.
    GeneIDi9705394.
    KEGGimmg:MTBMA_c16830.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001710 Genomic DNA. Translation: ADL59254.1 .
    RefSeqi YP_003850567.1. NC_014408.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZFS electron microscopy 4.00 C 1-281 [» ]
    4CI0 electron microscopy 3.36 C 1-281 [» ]
    4OMF X-ray 1.71 B 1-281 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL59254 ; ADL59254 ; MTBMA_c16830 .
    GeneIDi 9705394.
    KEGGi mmg:MTBMA_c16830.

    Phylogenomic databases

    HOGENOMi HOG000223731.
    KOi K00441.
    OMAi MENFPYN.

    Enzyme and pathway databases

    BioCyci MMAR79929:GH5J-1689-MONOMER.

    Family and domain databases

    InterProi IPR007516. Co_F420_Hydgase/DH_bsu_N.
    IPR017679. Coenz_F420_hydrogenase_bsu.
    IPR007525. FrhB_FdhB_C.
    [Graphical view ]
    Pfami PF04432. FrhB_FdhB_C. 1 hit.
    PF04422. FrhB_FdhB_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03289. frhB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Methanothermobacter marburgensis."
      Kaster A., Seedorf H., Goenrich M., Wiezer A., Liesegang H., Thauer R., Gottschalk G.
      Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Marburg.
    2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
      Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
      J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / MarburgImported.
    3. "De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy."
      Mills D.J., Vitt S., Strauss M., Shima S., Vonck J.
      Elife 2:e00218-e00218(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (4.00 ANGSTROMS) IN COMPLEX WITH FAD AND IRON-SULFUR(4FE-4S).
    4. "Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector."
      Allegretti M., Mills D.J., McMullan G., Kuhlbrandt W., Vonck J.
      Elife 3:e01963-e01963(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (3.36 ANGSTROMS) IN COMPLEX WITH FAD AND IRON-SULFUR(4FE-4S).

    Entry informationi

    Entry nameiD9PYF6_METTM
    AccessioniPrimary (citable) accession number: D9PYF6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteomeImported

    External Data

    Dasty 3