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D9PXP0

- D9PXP0_METTM

UniProt

D9PXP0 - D9PXP0_METTM

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Protein

Coenzyme F420:L-glutamate ligase

Gene
cofE, MTBMA_c14010
Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives By similarity.UniRule annotation

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

Cofactori

Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese By similarity.UniRule annotation
Monovalent cation. The ion could be potassium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501GTP By similarityUniRule annotation
Metal bindingi115 – 1151Divalent metal cation 1 By similarityUniRule annotation
Binding sitei118 – 1181GTP By similarityUniRule annotation
Metal bindingi156 – 1561Divalent metal cation 1 By similarityUniRule annotation
Metal bindingi157 – 1571Divalent metal cation 2 By similarityUniRule annotation
Metal bindingi214 – 2141Divalent metal cation 2 By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 144GTP By similarityUniRule annotation
Nucleotide bindingi45 – 462GTP By similarityUniRule annotation
Nucleotide bindingi212 – 2198GTP By similarityUniRule annotation

GO - Molecular functioni

  1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
  2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
  3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
  2. F420-0 metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1405-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligase
Coenzyme F420-1:gamma-L-glutamate ligase
Gene namesi
Name:cofEUniRule annotationImported
Ordered Locus Names:MTBMA_c14010Imported
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)Imported
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345: Chromosome

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the CofE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000223628.
KOiK12234.
OMAiTRIVENR.

Family and domain databases

HAMAPiMF_01258. F420_ligase_CofE.
InterProiIPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR023659. F420_ligase_CofE_arc.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.

Sequencei

Sequence statusi: Complete.

D9PXP0-1 [UniParc]FASTAAdd to Basket

« Hide

MEITLTGVEG LPLVSEGDDI AALIVDALKS SNMELLDGDI VVIAETLVAK    50
AEGNTVDLRR IEPSSKALEI ASRTGKDPRL VEAILEESSE ILKVGHDFIV 100
SETRHGFVCA NAGIDESNVE EGLATPLPED PDGSARRILG ALQELTGREM 150
AVIISDTQGR PFREGAVGVA VGVAGISPVW DRKGELDLYG RSLQTTRVAV 200
ADELAAAASL VMGQADEGVP AVIVRGYPWG HLRATGSARE LLRPRELDVF 250
RD 252
Length:252
Mass (Da):26,785
Last modified:October 5, 2010 - v1
Checksum:i69CB8BD43A19A2FC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001710 Genomic DNA. Translation: ADL58988.1.
RefSeqiYP_003850301.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL58988; ADL58988; MTBMA_c14010.
GeneIDi9705110.
KEGGimmg:MTBMA_c14010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001710 Genomic DNA. Translation: ADL58988.1 .
RefSeqi YP_003850301.1. NC_014408.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADL58988 ; ADL58988 ; MTBMA_c14010 .
GeneIDi 9705110.
KEGGi mmg:MTBMA_c14010.

Phylogenomic databases

HOGENOMi HOG000223628.
KOi K12234.
OMAi TRIVENR.

Enzyme and pathway databases

UniPathwayi UPA00071 .
BioCyci MMAR79929:GH5J-1405-MONOMER.

Family and domain databases

HAMAPi MF_01258. F420_ligase_CofE.
InterProi IPR002847. F420-0_gamma-glut_ligase-dom.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR023659. F420_ligase_CofE_arc.
[Graphical view ]
Pfami PF01996. F420_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01916. F420_cofE. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Methanothermobacter marburgensis."
    Kaster A., Seedorf H., Goenrich M., Wiezer A., Liesegang H., Thauer R., Gottschalk G.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Entry informationi

Entry nameiD9PXP0_METTM
AccessioniPrimary (citable) accession number: D9PXP0
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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