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D9PXP0

- D9PXP0_METTM

UniProt

D9PXP0 - D9PXP0_METTM

Protein

Coenzyme F420:L-glutamate ligase

Gene

cofE

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives.UniRule annotation

    Catalytic activityi

    GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
    GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

    Cofactori

    Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation
    Monovalent cation. The ion could be potassium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501GTPUniRule annotation
    Metal bindingi115 – 1151Divalent metal cation 1UniRule annotation
    Binding sitei118 – 1181GTPUniRule annotation
    Metal bindingi156 – 1561Divalent metal cation 1UniRule annotation
    Metal bindingi157 – 1571Divalent metal cation 2UniRule annotation
    Metal bindingi214 – 2141Divalent metal cation 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 144GTPUniRule annotation
    Nucleotide bindingi45 – 462GTPUniRule annotation
    Nucleotide bindingi212 – 2198GTPUniRule annotation

    GO - Molecular functioni

    1. coenzyme F420-0:L-glutamate ligase activity Source: UniProtKB-HAMAP
    2. coenzyme F420-0 gamma-glutamyl ligase activity Source: InterPro
    3. coenzyme F420-1:gamma-L-glutamate ligase activity Source: UniProtKB-HAMAP
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. coenzyme biosynthetic process Source: UniProtKB-HAMAP
    2. F420-0 metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

    Enzyme and pathway databases

    BioCyciMMAR79929:GH5J-1405-MONOMER.
    UniPathwayiUPA00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
    Alternative name(s):
    Coenzyme F420-0:L-glutamate ligaseUniRule annotation
    Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
    Gene namesi
    Name:cofEUniRule annotationImported
    Ordered Locus Names:MTBMA_c14010Imported
    OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)Imported
    Taxonomic identifieri79929 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000000345: Chromosome

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CofE family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000223628.
    KOiK12234.
    OMAiTRIVENR.

    Family and domain databases

    HAMAPiMF_01258. F420_ligase_CofE.
    InterProiIPR002847. F420-0_gamma-glut_ligase-dom.
    IPR008225. F420-0_gamma-glutamyl_ligase.
    IPR023659. F420_ligase_CofE_arc.
    [Graphical view]
    PfamiPF01996. F420_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01916. F420_cofE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D9PXP0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEITLTGVEG LPLVSEGDDI AALIVDALKS SNMELLDGDI VVIAETLVAK    50
    AEGNTVDLRR IEPSSKALEI ASRTGKDPRL VEAILEESSE ILKVGHDFIV 100
    SETRHGFVCA NAGIDESNVE EGLATPLPED PDGSARRILG ALQELTGREM 150
    AVIISDTQGR PFREGAVGVA VGVAGISPVW DRKGELDLYG RSLQTTRVAV 200
    ADELAAAASL VMGQADEGVP AVIVRGYPWG HLRATGSARE LLRPRELDVF 250
    RD 252
    Length:252
    Mass (Da):26,785
    Last modified:October 5, 2010 - v1
    Checksum:i69CB8BD43A19A2FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001710 Genomic DNA. Translation: ADL58988.1.
    RefSeqiYP_003850301.1. NC_014408.1.

    Genome annotation databases

    EnsemblBacteriaiADL58988; ADL58988; MTBMA_c14010.
    GeneIDi9705110.
    KEGGimmg:MTBMA_c14010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001710 Genomic DNA. Translation: ADL58988.1 .
    RefSeqi YP_003850301.1. NC_014408.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL58988 ; ADL58988 ; MTBMA_c14010 .
    GeneIDi 9705110.
    KEGGi mmg:MTBMA_c14010.

    Phylogenomic databases

    HOGENOMi HOG000223628.
    KOi K12234.
    OMAi TRIVENR.

    Enzyme and pathway databases

    UniPathwayi UPA00071 .
    BioCyci MMAR79929:GH5J-1405-MONOMER.

    Family and domain databases

    HAMAPi MF_01258. F420_ligase_CofE.
    InterProi IPR002847. F420-0_gamma-glut_ligase-dom.
    IPR008225. F420-0_gamma-glutamyl_ligase.
    IPR023659. F420_ligase_CofE_arc.
    [Graphical view ]
    Pfami PF01996. F420_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01916. F420_cofE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Methanothermobacter marburgensis."
      Kaster A., Seedorf H., Goenrich M., Wiezer A., Liesegang H., Thauer R., Gottschalk G.
      Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Marburg.
    2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
      Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
      J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / MarburgImported.

    Entry informationi

    Entry nameiD9PXP0_METTM
    AccessioniPrimary (citable) accession number: D9PXP0
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3