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Protein

Coenzyme F420:L-glutamate ligase

Gene

cofE

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives.UniRule annotation

Catalytic activityi

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1.UniRule annotation
GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • K(+)UniRule annotationNote: Monovalent cation. The ion could be potassium.UniRule annotation
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501GTPUniRule annotation
Metal bindingi115 – 1151Divalent metal cation 1UniRule annotation
Binding sitei118 – 1181GTPUniRule annotation
Metal bindingi156 – 1561Divalent metal cation 1UniRule annotation
Metal bindingi157 – 1571Divalent metal cation 2UniRule annotation
Metal bindingi214 – 2141Divalent metal cation 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 144GTPUniRule annotation
Nucleotide bindingi45 – 462GTPUniRule annotation
Nucleotide bindingi212 – 2198GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

GTP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciMMAR79929:GH5J-1405-MONOMER.
UniPathwayiUPA00071.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420:L-glutamate ligaseUniRule annotation (EC:6.3.2.31UniRule annotation, EC:6.3.2.34UniRule annotation)
Alternative name(s):
Coenzyme F420-0:L-glutamate ligaseUniRule annotation
Coenzyme F420-1:gamma-L-glutamate ligaseUniRule annotation
Gene namesi
Name:cofEUniRule annotationImported
Ordered Locus Names:MTBMA_c14010Imported
OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)Imported
Taxonomic identifieri79929 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
ProteomesiUP000000345 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD9PXP0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CofE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000223628.
KOiK12234.
OMAiTQGRPFR.

Family and domain databases

HAMAPiMF_01258. F420_ligase_CofE.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR023659. F420_ligase_CofE_arc.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.

Sequencei

Sequence statusi: Complete.

D9PXP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEITLTGVEG LPLVSEGDDI AALIVDALKS SNMELLDGDI VVIAETLVAK
60 70 80 90 100
AEGNTVDLRR IEPSSKALEI ASRTGKDPRL VEAILEESSE ILKVGHDFIV
110 120 130 140 150
SETRHGFVCA NAGIDESNVE EGLATPLPED PDGSARRILG ALQELTGREM
160 170 180 190 200
AVIISDTQGR PFREGAVGVA VGVAGISPVW DRKGELDLYG RSLQTTRVAV
210 220 230 240 250
ADELAAAASL VMGQADEGVP AVIVRGYPWG HLRATGSARE LLRPRELDVF

RD
Length:252
Mass (Da):26,785
Last modified:October 5, 2010 - v1
Checksum:i69CB8BD43A19A2FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001710 Genomic DNA. Translation: ADL58988.1.
RefSeqiWP_013296200.1. NC_014408.1.
YP_003850301.1. NC_014408.1.

Genome annotation databases

EnsemblBacteriaiADL58988; ADL58988; MTBMA_c14010.
GeneIDi9705110.
KEGGimmg:MTBMA_c14010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001710 Genomic DNA. Translation: ADL58988.1.
RefSeqiWP_013296200.1. NC_014408.1.
YP_003850301.1. NC_014408.1.

3D structure databases

ProteinModelPortaliD9PXP0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADL58988; ADL58988; MTBMA_c14010.
GeneIDi9705110.
KEGGimmg:MTBMA_c14010.

Phylogenomic databases

HOGENOMiHOG000223628.
KOiK12234.
OMAiTQGRPFR.

Enzyme and pathway databases

UniPathwayiUPA00071.
BioCyciMMAR79929:GH5J-1405-MONOMER.

Family and domain databases

HAMAPiMF_01258. F420_ligase_CofE.
InterProiIPR008225. F420-0_g-glutamyl_ligase.
IPR002847. F420-0_gamma-glut_ligase-dom.
IPR023659. F420_ligase_CofE_arc.
[Graphical view]
PfamiPF01996. F420_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01916. F420_cofE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Methanothermobacter marburgensis."
    Kaster A., Seedorf H., Goenrich M., Wiezer A., Liesegang H., Thauer R., Gottschalk G.
    Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Marburg.
  2. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
    Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
    J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / MarburgImported.

Entry informationi

Entry nameiD9PXP0_METTM
AccessioniPrimary (citable) accession number: D9PXP0
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: May 27, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.