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D9PXP0 (D9PXP0_METTM) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Coenzyme F420:L-glutamate ligase HAMAP-Rule MF_01258
EC=6.3.2.31 HAMAP-Rule MF_01258
EC=6.3.2.34 HAMAP-Rule MF_01258
Alternative name(s):
Coenzyme F420-0:L-glutamate ligase HAMAP-Rule MF_01258
Coenzyme F420-1:gamma-L-glutamate ligase HAMAP-Rule MF_01258
Gene names
Name:cofE HAMAP-Rule MF_01258 EMBL ADL58988.1
Ordered Locus Names:MTBMA_c14010 EMBL ADL58988.1
OrganismMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP] EMBL ADL58988.1
Taxonomic identifier79929 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter ›

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives By similarity. HAMAP-Rule MF_01258

Catalytic activity

GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1. HAMAP-Rule MF_01258

GTP + coenzyme F420-1 + L-glutamate = GDP + phosphate + coenzyme gamma-F420-2. HAMAP-Rule MF_01258

Cofactor

Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese By similarity. HAMAP-Rule MF_01258

Monovalent cation. The ion could be potassium By similarity. HAMAP-Rule MF_01258

Pathway

Cofactor biosynthesis; coenzyme F420 biosynthesis. HAMAP-Rule MF_01258

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01258

Sequence similarities

Belongs to the CofE family. HAMAP-Rule MF_01258

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding11 – 144GTP By similarity HAMAP-Rule MF_01258
Nucleotide binding45 – 462GTP By similarity HAMAP-Rule MF_01258
Nucleotide binding212 – 2198GTP By similarity HAMAP-Rule MF_01258

Sites

Metal binding1151Divalent metal cation 1 By similarity HAMAP-Rule MF_01258
Metal binding1561Divalent metal cation 1 By similarity HAMAP-Rule MF_01258
Metal binding1571Divalent metal cation 2 By similarity HAMAP-Rule MF_01258
Metal binding2141Divalent metal cation 2 By similarity HAMAP-Rule MF_01258
Binding site501GTP By similarity HAMAP-Rule MF_01258
Binding site1181GTP By similarity HAMAP-Rule MF_01258

Sequences

Sequence LengthMass (Da)Tools
D9PXP0 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: 69CB8BD43A19A2FC

FASTA25226,785
        10         20         30         40         50         60 
MEITLTGVEG LPLVSEGDDI AALIVDALKS SNMELLDGDI VVIAETLVAK AEGNTVDLRR 

        70         80         90        100        110        120 
IEPSSKALEI ASRTGKDPRL VEAILEESSE ILKVGHDFIV SETRHGFVCA NAGIDESNVE 

       130        140        150        160        170        180 
EGLATPLPED PDGSARRILG ALQELTGREM AVIISDTQGR PFREGAVGVA VGVAGISPVW 

       190        200        210        220        230        240 
DRKGELDLYG RSLQTTRVAV ADELAAAASL VMGQADEGVP AVIVRGYPWG HLRATGSARE 

       250 
LLRPRELDVF RD

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Methanothermobacter marburgensis."
Kaster A., Seedorf H., Goenrich M., Wiezer A., Liesegang H., Thauer R., Gottschalk G.
Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Marburg.
[2]"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001710 Genomic DNA. Translation: ADL58988.1.
RefSeqYP_003850301.1. NC_014408.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL58988; ADL58988; MTBMA_c14010.
GeneID9705110.
KEGGmmg:MTBMA_c14010.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000223628.
KOK12234.
OMATQGRPFR.

Enzyme and pathway databases

BioCycMMAR79929:GH5J-1409-MONOMER.
UniPathwayUPA00071.

Family and domain databases

HAMAPMF_01258. F420_ligase_CofE.
InterProIPR002847. F420-0_gamma-glut_ligase-rel.
IPR008225. F420-0_gamma-glutamyl_ligase.
IPR023659. F420_ligase_CofE_arc.
[Graphical view]
PfamPF01996. F420_ligase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01916. F420_cofE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD9PXP0_METTM
AccessionPrimary (citable) accession number: D9PXP0
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: May 1, 2013
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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