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D9PW35 (D9PW35_METTM) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UvrABC system protein B HAMAP-Rule MF_00204
Short name=Protein UvrB HAMAP-Rule MF_00204
Alternative name(s):
Excinuclease ABC subunit B HAMAP-Rule MF_00204
Gene names
Name:uvrB HAMAP-Rule MF_00204 EMBL ADL58433.1
Ordered Locus Names:MTBMA_c08380 EMBL ADL58433.1
OrganismMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP] EMBL ADL58433.1
Taxonomic identifier79929 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter ›

Protein attributes

Sequence length647 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity. RuleBase RU003587 HAMAP-Rule MF_00204

Subcellular location

Cytoplasm By similarity RuleBase RU003587 HAMAP-Rule MF_00204.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family. RuleBase RU003587 HAMAP-Rule MF_00204

Contains 1 UVR domain. RuleBase RU003587 HAMAP-Rule MF_00204

Contains 1 helicase ATP-binding domain. HAMAP-Rule MF_00204

Contains 1 helicase C-terminal domain. HAMAP-Rule MF_00204

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain24 – 184161Helicase ATP-binding By similarity HAMAP-Rule MF_00204
Domain427 – 589163Helicase C-terminal By similarity HAMAP-Rule MF_00204
Domain609 – 64436UVR By similarity HAMAP-Rule MF_00204
Nucleotide binding37 – 448ATP By similarity HAMAP-Rule MF_00204
Motif90 – 11324Beta-hairpin By similarity HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
D9PW35 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: 3FC0AB1D2BD2EE1D

FASTA64774,643
        10         20         30         40         50         60 
MKFKLVSDYR PLGDQPKAIR SLVEGINAGM REQTLLGVTG SGKTFTIANV IEEVQKPTLV 

        70         80         90        100        110        120 
ISHNKTLAAQ LYEEFREFFP DNAVEYFVSY YDFYQPEAYI PQTDTYIDKE ASINDEIDRM 

       130        140        150        160        170        180 
RHSATQALLS RDDVIVVSSV SCIYGIGAPA DYGEFTLHLE VGSSIGREEI LSSLVSMQYE 

       190        200        210        220        230        240 
RNDVEFDRGQ FRVRGDTIEI NPIHGTPPIR IELFGDEVDS ISTVHRVTGK RLQKLDRITV 

       250        260        270        280        290        300 
FPAKHFVIPE DRLQRAIESI EEELEDRLRE LRAQNKLLEA QRLEQRTRFD IEMLREMGYC 

       310        320        330        340        350        360 
QGIENYSMHL SGRKWGEKPN TLLDYFPDDF LTVIDESHVT VPQIRGMYNG DRARKETLVE 

       370        380        390        400        410        420 
YGFRLPSAKE NRPLRFDEFQ ESINQVIYVS ATPGKYELSR SQNIVEQIIR PTGLVDPEVR 

       430        440        450        460        470        480 
IRPVKGQVDD LLSEIRRRVE LGQRVLVTTL TKRMAEDLTD YYSRVGVRVR YLHSEIDTLE 

       490        500        510        520        530        540 
RVEIIDDLRR GEFDCLVGVN LLREGLDLPE VSLVAILDAD REGFLRSETS LIQTIGRAAR 

       550        560        570        580        590        600 
NVNGQVLIYA DRLTDAVKAA VETTNRRRKL QMEYNRRHGI KPRSTRRTLR EKKEKEEIKA 

       610        620        630        640 
EEIPRDELEV IIKDLEAEMR EAARNLEFER AARLRDQIMS LKGSSSK

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Methanothermobacter marburgensis."
Kaster A., Seedorf H., Goenrich M., Wiezer A., Liesegang H., Thauer R., Gottschalk G.
Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: Marburg.
[2]"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001710 Genomic DNA. Translation: ADL58433.1.
RefSeqYP_003849746.1. NC_014408.1.

3D structure databases

ProteinModelPortalD9PW35.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL58433; ADL58433; MTBMA_c08380.
GeneID9704546.
KEGGmmg:MTBMA_c08380.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000073580.
KOK03702.
OMACIYGLGI.

Enzyme and pathway databases

BioCycMMAR79929:GH5J-841-MONOMER.

Family and domain databases

Gene3D4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF46600. UvrB_C. 1 hit.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD9PW35_METTM
AccessionPrimary (citable) accession number: D9PW35
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: May 29, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info