Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

D9N170

- D9N170_PLAFA

UniProt

D9N170 - D9N170_PLAFA

Protein

Bifunctional dihydrofolate reductase-thymidylate synthase

Gene

DHFR-TS

Organism
Plasmodium falciparum
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (05 Oct 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei16 – 161NADP; via amide nitrogen and carbonyl oxygenImported
    Binding sitei40 – 401NADP; via carbonyl oxygenImported
    Binding sitei144 – 1441NADP; via carbonyl oxygenImported
    Active sitei490 – 4901UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi44 – 452NADPImported
    Nucleotide bindingi106 – 1083NADPImported
    Nucleotide bindingi127 – 1304NADPImported
    Nucleotide bindingi165 – 1728NADPImported

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: InterPro
    2. nucleotide binding Source: UniProtKB-KW
    3. thymidylate synthase activity Source: InterPro

    GO - Biological processi

    1. dTMP biosynthetic process Source: InterPro
    2. glycine biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    MethyltransferaseUniRule annotation, OxidoreductaseUniRule annotation, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesisUniRule annotation, One-carbon metabolismUniRule annotation

    Keywords - Ligandi

    NADPImported, Nucleotide-bindingImported

    Enzyme and pathway databases

    ReactomeiREACT_192655. G1/S-Specific Transcription.
    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional dihydrofolate reductase-thymidylate synthaseUniRule annotation
    Gene namesi
    Name:DHFR-TSImported
    ORF Names:V1/SImported
    OrganismiPlasmodium falciparumImported
    Taxonomic identifieri5833 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiumPlasmodium (Laverania)

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3JSUX-ray2.70A/B1-608[»]
    3QG2X-ray2.30A/B1-608[»]
    4DP3X-ray2.40A/B1-608[»]
    4DPHX-ray2.38A/B1-608[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiD9N170.

    Family & Domainsi

    Sequence similaritiesi

    In the C-terminal section; belongs to the thymidylate synthase family.UniRule annotation
    In the N-terminal section; belongs to the dihydrofolate reductase family.UniRule annotation

    Family and domain databases

    Gene3Di3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPiMF_00008. Thymidy_synth_bact.
    InterProiIPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000389. DHFR-TS. 1 hit.
    PRINTSiPR00108. THYMDSNTHASE.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsiTIGR03284. thym_sym. 1 hit.
    PROSITEiPS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D9N170-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMEQVCDVFD IYAICACCKV ESKNEGKKNE VFNNYTFRGL GNKGVLPWKC    50
    ISLDMKYFRA VTTYVNESKY EKLKYKRCKY LNKETVDNVN DMPNSKKLQN 100
    VVVMGRTNWE SIPKKFKPLS NRINVILSRT LKKEDFDEDV YIINKVEDLI 150
    VLLGKLNYYK CFILGGSVVY QEFLEKKLIK KIYFTRINST YECDVFFPEI 200
    NENEYQIISV SDVYTSNNTT LDFIIYKKTN NKMLNEQNCI KGEEKNNDMP 250
    LKNDDKDTCH MKKLTEFYKN VDKYKINYEN DDDDEEEDDF VYFNFNKEKE 300
    EKNKNSIHPN DFQIYNSLKY KYHPEYQYLN IIYDIMMNGN KQSDRTGVGV 350
    LSKFGYIMKF DLSQYFPLLT TKKLFLRGII EELLWFIRGE TNGNTLLNKN 400
    VRIWEANGTR EFLDNRKLFH REVNDLGPIY GFQWRHFGAE YTNMYDNYEN 450
    KGVDQLKNII NLIKNDPTSR RILLCAWNVK DLDQMALPPC HILCQFYVFD 500
    GKLSCIMYQR SCDLGLGVPF NIASYSIFTH MIAQVCNLQP AQFIHVLGNA 550
    HVYNNHIDSL KIQLNRIPYP FPTLKLNPDI KNIEDFTISD FTIQNYVHHE 600
    KISMDMAA 608
    Length:608
    Mass (Da):71,816
    Last modified:October 5, 2010 - v1
    Checksum:i544ED69133C63613
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3JSU X-ray 2.70 A/B 1-608 [» ]
    3QG2 X-ray 2.30 A/B 1-608 [» ]
    4DP3 X-ray 2.40 A/B 1-608 [» ]
    4DPH X-ray 2.38 A/B 1-608 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    Reactomei REACT_192655. G1/S-Specific Transcription.

    Miscellaneous databases

    EvolutionaryTracei D9N170.

    Family and domain databases

    Gene3Di 3.30.572.10. 1 hit.
    3.40.430.10. 1 hit.
    HAMAPi MF_00008. Thymidy_synth_bact.
    InterProi IPR024072. DHFR-like_dom.
    IPR012262. DHFR-TS.
    IPR017925. DHFR_CS.
    IPR001796. DHFR_dom.
    IPR023451. Thymidate_synth/dCMP_Mease.
    IPR000398. Thymidylate_synthase.
    IPR020940. Thymidylate_synthase_AS.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    PF00303. Thymidylat_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000389. DHFR-TS. 1 hit.
    PRINTSi PR00108. THYMDSNTHASE.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    SSF55831. SSF55831. 1 hit.
    TIGRFAMsi TIGR03284. thym_sym. 1 hit.
    PROSITEi PS00075. DHFR_1. 1 hit.
    PS51330. DHFR_2. 1 hit.
    PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Preclinical evaluation of the antifolate QN254, 5-chloro- N'6'-(2,5-dimethoxy-benzyl)-quinazoline-2,4,6-triamine, as an antimalarial drug candidate."
      Nzila A., Rottmann M., Chitnumsub P., Kiara S.M., Kamchonwongpaisan S., Maneeruttanarungroj C., Taweechai S., Yeung B.K., Goh A., Lakshminarayana S.B., Zou B., Wong J., Ma N.L., Weaver M., Keller T.H., Dartois V., Wittlin S., Brun R., Yuthavong Y., Diagana T.T.
      Antimicrob. Agents Chemother. 54:2603-2610(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) IN COMPLEX WITH NADP.
    2. "Trypanosomal dihydrofolate reductase reveals natural antifolate resistance."
      Vanichtanankul J., Taweechai S., Yuvaniyama J., Vilaivan T., Chitnumsub P., Kamchonwongpaisan S., Yuthavong Y.
      ACS Chem. Biol. 6:905-911(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH NADP.
    3. Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) IN COMPLEX WITH NADP.

    Entry informationi

    Entry nameiD9N170_PLAFA
    AccessioniPrimary (citable) accession number: D9N170
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3