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Protein

DNA polymerase

Gene

DPO1

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: UniProtKB-KW
  3. DNA-directed DNA polymerase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA-dependent DNA replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

NucleotidyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

DNA replicationUniRule annotation

Keywords - Ligandi

DNA-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymeraseUniRule annotation (EC:2.7.7.7UniRule annotation)
Gene namesi
Name:DPO1Imported
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HT3X-ray1.70A/D1-579[»]
4O0IX-ray2.20A1-579[»]
ProteinModelPortaliD9N168.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiD9N168.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.UniRule annotation

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D9N168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
KMAFTLADRV TEEMLADKAA LVVEVVEENY HDAPIVGIAV VNEHGRFFLR
60 70 80 90 100
PETALADPQF VAWLGDETKK KSMFDSKRAA VALKWKGIEL CGVSFDLLLA
110 120 130 140 150
AYLLDPAQGV DDVAAAAKMK QYEAVRPDEA VYGKGAKRAV PDEPVLAEHL
160 170 180 190 200
VRKAAAIWEL ERPFLDELRR NEQDRLLVEL EQPLSSILAE MEFAGVKVDT
210 220 230 240 250
KRLEQMGKEL AEQLGTVEQR IYELAGQEFN INSPKQLGVI LFEKLQLPVL
260 270 280 290 300
KKTKTGYSTS ADVLEKLAPY HEIVENILHY RQLGKLQSTY IEGLLKVVRP
310 320 330 340 350
ATKKVHTIFN QALTQTGRLS STEPNLQNIP IRLEEGRKIR QAFVPSESDW
360 370 380 390 400
LIFAADYSQI ELRVLAHIAE DDNLMEAFRR DLDIHTKTAM DIFQVSEDEV
410 420 430 440 450
TPNMRRQAKA VNFGIPYGIS DYGLAQNLNI SRKEAAEFIE RYFESFPGVK
460 470 480 490 500
RYMENIVQEA KQKGYVTTLL HRRRYLPDIT SRNFNVRSFA ERMAMNTPIQ
510 520 530 540 550
GSAADIIKKA MIDLNARLKE ERLQAHLLLQ VHDELILEAP KEEMERLCRL
560 570
VPEVMEQAVT LRVPLKVDYH YGSTWYDAK
Length:579
Mass (Da):65,967
Last modified:October 4, 2010 - v1
Checksum:iCDEDB059CBA01889
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HT3X-ray1.70A/D1-579[»]
4O0IX-ray2.20A1-579[»]
ProteinModelPortaliD9N168.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiD9N168.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR002562. 3'-5'_exonuclease_dom.
IPR019760. DNA-dir_DNA_pol_A_CS.
IPR001098. DNA-dir_DNA_pol_A_palm_dom.
IPR018320. DNA_polymerase_1.
IPR002298. DNA_polymerase_A.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00476. DNA_pol_A. 1 hit.
[Graphical view]
PRINTSiPR00868. DNAPOLI.
SMARTiSM00474. 35EXOc. 1 hit.
SM00482. POLAc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00593. pola. 1 hit.
PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "ajar" intermediate conformation in the nucleotide selection mechanism."
    Wu E.Y., Beese L.S.
    J. Biol. Chem. 286:19758-19767(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).
  2. "Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with 2'-MeSe-arabino-guanosine derivatized DNA."
    Jiang S., Gan J., Sun H., Huang Z.
    Submitted (NOV-2013) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).

Entry informationi

Entry nameiD9N168_GEOSE
AccessioniPrimary (citable) accession number: D9N168
Entry historyi
Integrated into UniProtKB/TrEMBL: October 4, 2010
Last sequence update: October 4, 2010
Last modified: March 3, 2015
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sourcesImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.