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D9N168

- D9N168_GEOSE

UniProt

D9N168 - D9N168_GEOSE

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Protein
DNA polymerase
Gene
DPO1
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

GO - Molecular functioni

  1. 3'-5' exonuclease activity Source: InterPro
  2. DNA binding Source: UniProtKB-KW
  3. DNA-directed DNA polymerase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    NucleotidyltransferaseUniRule annotation, Transferase

    Keywords - Biological processi

    DNA replicationUniRule annotation

    Keywords - Ligandi

    DNA-bindingUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymeraseUniRule annotation (EC:2.7.7.7UniRule annotation)
    Gene namesi
    Name:DPO1Imported
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3HT3X-ray1.70A/D1-579[»]
    ProteinModelPortaliD9N168.

    Miscellaneous databases

    EvolutionaryTraceiD9N168.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    InterProiIPR002562. 3'-5'_exonuclease_dom.
    IPR019760. DNA-dir_DNA_pol_A_CS.
    IPR001098. DNA-dir_DNA_pol_A_palm_dom.
    IPR018320. DNA_polymerase_1.
    IPR002298. DNA_polymerase_A.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00476. DNA_pol_A. 1 hit.
    [Graphical view]
    PRINTSiPR00868. DNAPOLI.
    SMARTiSM00474. 35EXOc. 1 hit.
    SM00482. POLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00593. pola. 1 hit.
    PROSITEiPS00447. DNA_POLYMERASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D9N168-1 [UniParc]FASTAAdd to Basket

    « Hide

    KMAFTLADRV TEEMLADKAA LVVEVVEENY HDAPIVGIAV VNEHGRFFLR    50
    PETALADPQF VAWLGDETKK KSMFDSKRAA VALKWKGIEL CGVSFDLLLA 100
    AYLLDPAQGV DDVAAAAKMK QYEAVRPDEA VYGKGAKRAV PDEPVLAEHL 150
    VRKAAAIWEL ERPFLDELRR NEQDRLLVEL EQPLSSILAE MEFAGVKVDT 200
    KRLEQMGKEL AEQLGTVEQR IYELAGQEFN INSPKQLGVI LFEKLQLPVL 250
    KKTKTGYSTS ADVLEKLAPY HEIVENILHY RQLGKLQSTY IEGLLKVVRP 300
    ATKKVHTIFN QALTQTGRLS STEPNLQNIP IRLEEGRKIR QAFVPSESDW 350
    LIFAADYSQI ELRVLAHIAE DDNLMEAFRR DLDIHTKTAM DIFQVSEDEV 400
    TPNMRRQAKA VNFGIPYGIS DYGLAQNLNI SRKEAAEFIE RYFESFPGVK 450
    RYMENIVQEA KQKGYVTTLL HRRRYLPDIT SRNFNVRSFA ERMAMNTPIQ 500
    GSAADIIKKA MIDLNARLKE ERLQAHLLLQ VHDELILEAP KEEMERLCRL 550
    VPEVMEQAVT LRVPLKVDYH YGSTWYDAK 579
    Length:579
    Mass (Da):65,967
    Last modified:October 5, 2010 - v1
    Checksum:iCDEDB059CBA01889
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3HT3 X-ray 1.70 A/D 1-579 [» ]
    ProteinModelPortali D9N168.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei D9N168.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    InterProi IPR002562. 3'-5'_exonuclease_dom.
    IPR019760. DNA-dir_DNA_pol_A_CS.
    IPR001098. DNA-dir_DNA_pol_A_palm_dom.
    IPR018320. DNA_polymerase_1.
    IPR002298. DNA_polymerase_A.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00476. DNA_pol_A. 1 hit.
    [Graphical view ]
    PRINTSi PR00868. DNAPOLI.
    SMARTi SM00474. 35EXOc. 1 hit.
    SM00482. POLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00593. pola. 1 hit.
    PROSITEi PS00447. DNA_POLYMERASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "ajar" intermediate conformation in the nucleotide selection mechanism."
      Wu E.Y., Beese L.S.
      J. Biol. Chem. 286:19758-19767(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS).

    Entry informationi

    Entry nameiD9N168_GEOSE
    AccessioniPrimary (citable) accession number: D9N168
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: April 16, 2014
    This is version 19 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3

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