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Protein
Submitted name:

Ribosome inactivating protein

Gene

RIP

Organism
Momordica balsamina (Bitter gourd) (Balsam apple)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70FructoseCombined sources1
Binding sitei85Beta-D-mannoseCombined sources1
Binding sitei85CDPCombined sources1
Binding sitei85CTPCombined sources1
Binding sitei85UDPCombined sources1
Binding sitei110Beta-D-mannose; via amide nitrogenCombined sources1
Binding sitei111AscorbateCombined sources1
Binding sitei122CTPCombined sources1
Binding sitei163CDPCombined sources1
Binding sitei163CTPCombined sources1
Binding sitei163FructoseCombined sources1
Binding sitei163UDPCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 71UDPCombined sources3
Nucleotide bindingi70 – 71CDPCombined sources2
Nucleotide bindingi70 – 71CTPCombined sources2
Nucleotide bindingi109 – 112CDPCombined sources4
Nucleotide bindingi109 – 112CTPCombined sources4
Nucleotide bindingi109 – 111UDPCombined sources3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseSAAS annotation, Protein synthesis inhibitorSAAS annotation, ToxinSAAS annotation

Keywords - Biological processi

Plant defenseSAAS annotation

Names & Taxonomyi

Protein namesi
Submitted name:
Ribosome inactivating proteinImported (EC:3.2.2.22Imported)
Gene namesi
Name:RIPImported
OrganismiMomordica balsamina (Bitter gourd) (Balsam apple)Imported
Taxonomic identifieri3672 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeMomordiceaeMomordica

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi227N-linked (GlcNAc...)Combined sources1

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MRWX-ray1.70A1-246[»]
3N1NX-ray2.23A1-246[»]
3N2DX-ray2.22A1-246[»]
3N31X-ray2.11A1-246[»]
3N3XX-ray1.70A1-246[»]
3N5DX-ray1.90A1-246[»]
3NFMX-ray2.50A1-246[»]
3NJSX-ray2.10A1-246[»]
3NX9X-ray1.70A1-246[»]
3Q4PX-ray1.80A1-246[»]
3QJIX-ray1.75A1-246[»]
3RL9X-ray1.90A1-246[»]
3S9QX-ray1.67A1-246[»]
3SJ6X-ray1.60A1-246[»]
3U6TX-ray1.85A1-246[»]
3U6ZX-ray1.70A1-246[»]
3U70X-ray2.00A1-246[»]
3U8FX-ray1.80A1-246[»]
3V14X-ray1.70A1-246[»]
3V2KX-ray2.07A1-246[»]
4DWMX-ray1.62A1-246[»]
4EMFX-ray1.77A1-246[»]
4EMRX-ray1.75A1-246[»]
4F9NX-ray2.65A1-246[»]
4FXAX-ray1.70A1-246[»]
4FZ9X-ray1.70A1-246[»]
4GUWX-ray1.60A1-246[»]
4H0ZX-ray2.00A1-246[»]
4HOAX-ray2.00A1-246[»]
4I47X-ray2.65A1-246[»]
4JTBX-ray1.71A1-246[»]
4JTPX-ray1.85A1-246[»]
4K2ZX-ray1.80A1-246[»]
4KL4X-ray1.90A1-246[»]
4KMKX-ray1.65A1-246[»]
4KPVX-ray2.57A1-246[»]
4KWNX-ray1.80A1-246[»]
4L66X-ray1.70A1-246[»]
4LROX-ray1.98A1-246[»]
4LT4X-ray1.69A1-246[»]
4LWXX-ray1.78A1-246[»]
4M5AX-ray1.70A1-246[»]
4O0OX-ray2.59A1-246[»]
4O4QX-ray1.81A1-246[»]
4O8EX-ray2.00A1-246[»]
4Q9FX-ray1.75A1-246[»]
4RZJX-ray1.98A1-246[»]
4XY7X-ray2.50A1-246[»]
4ZT8X-ray1.98A1-246[»]
4ZU0X-ray1.80A1-246[»]
4ZZ6X-ray2.00A1-246[»]
5CIXX-ray1.88A1-246[»]
5CSOX-ray1.78A1-246[»]
5CSTX-ray1.78A1-246[»]
5GM7X-ray1.78A1-246[»]
5ILWX-ray1.98A1-246[»]
5ILXX-ray1.70A1-246[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 71Ascorbate bindingCombined sources2
Regioni109 – 111Fructose bindingCombined sources3

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

D9J2T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DVSFRLSGAD PSSYGMFIKD LRNALPHTEK VYNIPLLLPS VSGAGRYLLM
60 70 80 90 100
HLFNYDGNTI TVAVDVTNVY IMGYLALTTS YFFNEPAADL ASQYVFRSAR
110 120 130 140 150
RKITLPYSGN YERLQIAAGK PREKIPIGLP ALDTAISTLL HYDSTAAAGA
160 170 180 190 200
LLVLIQTTAE AARFKYIEQQ IQERAYRDEV PSSATISLEN SWSGLSKQIQ
210 220 230 240
LAQGNNGVFR TPTVLVDSKG NRVQITNVTS NVVTSNIQLL LNTKNI
Length:246
Mass (Da):27,069
Last modified:October 5, 2010 - v1
Checksum:i6B38879C004BB72D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1Imported1
Non-terminal residuei246Imported1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM367595 mRNA. Translation: ADK23804.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM367595 mRNA. Translation: ADK23804.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MRWX-ray1.70A1-246[»]
3N1NX-ray2.23A1-246[»]
3N2DX-ray2.22A1-246[»]
3N31X-ray2.11A1-246[»]
3N3XX-ray1.70A1-246[»]
3N5DX-ray1.90A1-246[»]
3NFMX-ray2.50A1-246[»]
3NJSX-ray2.10A1-246[»]
3NX9X-ray1.70A1-246[»]
3Q4PX-ray1.80A1-246[»]
3QJIX-ray1.75A1-246[»]
3RL9X-ray1.90A1-246[»]
3S9QX-ray1.67A1-246[»]
3SJ6X-ray1.60A1-246[»]
3U6TX-ray1.85A1-246[»]
3U6ZX-ray1.70A1-246[»]
3U70X-ray2.00A1-246[»]
3U8FX-ray1.80A1-246[»]
3V14X-ray1.70A1-246[»]
3V2KX-ray2.07A1-246[»]
4DWMX-ray1.62A1-246[»]
4EMFX-ray1.77A1-246[»]
4EMRX-ray1.75A1-246[»]
4F9NX-ray2.65A1-246[»]
4FXAX-ray1.70A1-246[»]
4FZ9X-ray1.70A1-246[»]
4GUWX-ray1.60A1-246[»]
4H0ZX-ray2.00A1-246[»]
4HOAX-ray2.00A1-246[»]
4I47X-ray2.65A1-246[»]
4JTBX-ray1.71A1-246[»]
4JTPX-ray1.85A1-246[»]
4K2ZX-ray1.80A1-246[»]
4KL4X-ray1.90A1-246[»]
4KMKX-ray1.65A1-246[»]
4KPVX-ray2.57A1-246[»]
4KWNX-ray1.80A1-246[»]
4L66X-ray1.70A1-246[»]
4LROX-ray1.98A1-246[»]
4LT4X-ray1.69A1-246[»]
4LWXX-ray1.78A1-246[»]
4M5AX-ray1.70A1-246[»]
4O0OX-ray2.59A1-246[»]
4O4QX-ray1.81A1-246[»]
4O8EX-ray2.00A1-246[»]
4Q9FX-ray1.75A1-246[»]
4RZJX-ray1.98A1-246[»]
4XY7X-ray2.50A1-246[»]
4ZT8X-ray1.98A1-246[»]
4ZU0X-ray1.80A1-246[»]
4ZZ6X-ray2.00A1-246[»]
5CIXX-ray1.88A1-246[»]
5CSOX-ray1.78A1-246[»]
5CSTX-ray1.78A1-246[»]
5GM7X-ray1.78A1-246[»]
5ILWX-ray1.98A1-246[»]
5ILXX-ray1.70A1-246[»]
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiD9J2T9_MOMBA
AccessioniPrimary (citable) accession number: D9J2T9
Entry historyi
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: November 30, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.