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Protein

Endolysin

Gene

e

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.UniRule annotation

Catalytic activityi

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11Proton donorUniRule annotation1
Active sitei20NucleophileUniRule annotation1
Binding sitei32N-acetyl-D-glucosamine; via amide nitrogenUniRule annotation1
Binding sitei104N-acetyl-D-glucosamine; via carbonyl nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Bacteriolytic enzymeUniRule annotation, GlycosidaseUniRule annotation, Hydrolase
Biological processCytolysis, Host cell lysis by virusUniRule annotation, Virus exit from host cell

Protein family/group databases

CAZyiGH24. Glycoside Hydrolase Family 24.

Names & Taxonomyi

Protein namesi
Recommended name:
EndolysinUniRule annotation (EC:3.2.1.17UniRule annotation)
Alternative name(s):
Lysis proteinUniRule annotation
LysozymeUniRule annotation
MuramidaseUniRule annotation
Gene namesi
Name:eImported
ORF Names:T4Tp126Imported
OrganismiEnterobacteria phage T4 (Bacteriophage T4)Imported
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4virus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000001092 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation

  • Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PJZX-ray1.87A1-164[»]
4PK0X-ray2.30A1-164[»]
4PLAX-ray2.77A2-164[»]
4QKXX-ray3.30A2-161[»]
4U15X-ray2.80A/B61-161[»]
4U16X-ray3.70A/B61-161[»]
4UISelectron microscopy4.40G1-162[»]
4XSJX-ray1.80A1-161[»]
4YC4X-ray2.58A2-164[»]
5BZ6X-ray2.75A1-161[»]
5I0NX-ray2.28A2-164[»]
5NDDX-ray2.80A2-161[»]
5NDZX-ray3.60A2-161[»]
ProteinModelPortaliD9IEF7.
SMRiD9IEF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni45 – 48N-acetyl-D-glucosamine bindingCombined sources4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 24 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000CE.

Family and domain databases

Gene3Di1.10.530.40. 1 hit.
HAMAPiMF_04110. ENDOLYSIN_T4. 1 hit.
InterProiView protein in InterPro
IPR034690. Endolysin_T4_type.
IPR002196. Glyco_hydro_24.
IPR023346. Lysozyme-like_dom.
IPR023347. Lysozyme_dom.
IPR001165. T4-type_lysozyme.
PfamiView protein in Pfam
PF00959. Phage_lysozyme. 1 hit.
PRINTSiPR00684. T4LYSOZYME.
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

D9IEF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIFEMLRID EGLRLKIYKD TEGYYTIGIG HLLTKSPSLN AAKSELDKAI
60 70 80 90 100
GRNCNGVITK DEAEKLFNQD VDAAVRGILR NAKLKPVYDS LDAVRRCALI
110 120 130 140 150
NMVFQMGETG VAGFTNSLRM LQQKRWDEAA VNLAKSRWYN QTPNRAKRVI
160
TTFRTGTWDA YKNL
Length:164
Mass (Da):18,635
Last modified:October 5, 2010 - v1
Checksum:i6042E2F77B6308A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HM137666 Genomic DNA. Translation: ADJ39843.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiD9IEF7_BPT4
AccessioniPrimary (citable) accession number: D9IEF7
Entry historyiIntegrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: July 5, 2017
This is version 41 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteomeImported