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D8NXW9 (D8NXW9_RALSL) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlB EMBL CBJ50028.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:RPSI07_0665 EMBL CBJ50028.1
OrganismRalstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP] EMBL CBJ50028.1
Taxonomic identifier305 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. SAAS SAAS005905 HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. SAAS SAAS005905 HAMAP-Rule MF_00047

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. SAAS SAAS005905 HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity SAAS SAAS005905 HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. SAAS SAAS005905 HAMAP-Rule MF_00047

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain116 – 316201ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding142 – 19756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2691Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2831Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2831Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2851Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D8NXW9 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: 7FEFE1608AD5DB8D

FASTA33135,440
        10         20         30         40         50         60 
MTTGPFVPHP TLDPKSLGKV GVILGGRSAE REISLLSGNG VLAALRSRGV DAHPFDPGTQ 

        70         80         90        100        110        120 
PVAGLATQGF HRVVISLHGR FGEDGTIQGL LEQFGIPYTG SGVLASALAM DKEATKRLWQ 

       130        140        150        160        170        180 
THGLPTPDFV MLHAGADWQA VADRLGLPLI VKPAREGSSI GLTKVTSVAE LPAAYEKAAR 

       190        200        210        220        230        240 
LDRDVMAEQF IEGDELTCAV IGEGESATAL PLIRIVAPQA NYDYQHKYFT DDTRYECPAP 

       250        260        270        280        290        300 
IPADVAARVQ ALVVQAYRGL GCRGWGRADI MLREHDNAPF LLEMNTSPGM TGHSLVPMAA 

       310        320        330 
RAVGISYEDF VLQLAATASL ELRASHDWKP E

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References

[1]"Genomes of three tomato pathogens within the Ralstonia solanacearum species complex reveal significant evolutionary divergence."
Remenant B., Coupat-Goutaland B., Guidot A., Cellier G., Wicker E., Allen C., Fegan M., Pruvost O., Elbaz M., Calteau A., Salvignol G., Mornico D., Mangenot S., Barbe V., Medigue C., Prior P.
BMC Genomics 11:379-379(2010)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PSI07 EMBL CBJ50028.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FP885906 Genomic DNA. Translation: CBJ50028.1.
RefSeqYP_003751338.1. NC_014311.1.

3D structure databases

ProteinModelPortalD8NXW9.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBJ50028; CBJ50028; RPSI07_0665.
GeneID9410217.
KEGGrsl:RPSI07_0665.
PATRIC42537450. VBIRalSol167236_2336.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.

Enzyme and pathway databases

BioCycRSOL859657:GJJ9-671-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD8NXW9_RALSL
AccessionPrimary (citable) accession number: D8NXW9
Entry history
Integrated into UniProtKB/TrEMBL: October 5, 2010
Last sequence update: October 5, 2010
Last modified: April 3, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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